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The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide.
Eur J Nutr. 2015 Jun; 54(4):581-8.EJ

Abstract

PURPOSE

This study was conducted to investigate whether aspartate (Asp) could improve liver energy status in the lipopolysaccharide (LPS)-challenged pigs.

METHODS

Twenty-four weaned pigs were assigned to four treatments: (1) nonchallenged control (control diet and saline-treated); (2) LPS-challenged control (the same control diet and LPS-challenged); (3) LPS + 0.5% Asp treatment (0.5% Asp diet and LPS-challenged); and (4) LPS + 1.0% Asp treatment (a 1.0% Asp diet and LPS-challenged). On d 19, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weight, and the same volume of 0.9% NaCl solution, respectively. All pigs were slaughtered at 24 h after LPS or saline injection, and the liver was collected for further analysis.

RESULTS

Dietary supplementation with Asp improved liver energy status evidenced by the increased ATP concentration and adenylate energy charges, and the decreased AMP concentration and AMP/ATP ratio (p < 0.05). Asp supplementation increased the mRNA expression of key enzymes in hepatic glycolysis and tricarboxylic acid (TCA) cycle, including pyruvate kinase and citrate synthase (p < 0.05), and had a tendency to increase hepatic pyruvate dehydrogenase and isocitrate dehydrogenase β mRNA expression (p < 0.10). In addition, Asp increased the mRNA expressions of hepatic AMP-activated protein kinase (AMPK) α1, AMPKα2, silent information regulator (Sirt1), and proliferator-activated receptor-γ coactivator 1α (PGC1α) (p < 0.05). Moreover, Asp increased AMPKα phosphorylation (p < 0.05).

CONCLUSIONS

These results indicated that dietary supplementation of Asp could improve energy status in LPS-injured liver, which might result from motivating the metabolism pathway of TCA cycle and glycolysis and stimulating the AMPK signaling pathway.

Authors+Show Affiliations

Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China, ylkp2003@163.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25052542

Citation

Kang, Ping, et al. "The Effect of Aspartate On the Energy Metabolism in the Liver of Weanling Pigs Challenged With Lipopolysaccharide." European Journal of Nutrition, vol. 54, no. 4, 2015, pp. 581-8.
Kang P, Liu Y, Zhu H, et al. The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide. Eur J Nutr. 2015;54(4):581-8.
Kang, P., Liu, Y., Zhu, H., Li, S., Shi, H., Chen, F., Leng, W., Pi, D., Hou, Y., & Yi, D. (2015). The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide. European Journal of Nutrition, 54(4), 581-8. https://doi.org/10.1007/s00394-014-0739-3
Kang P, et al. The Effect of Aspartate On the Energy Metabolism in the Liver of Weanling Pigs Challenged With Lipopolysaccharide. Eur J Nutr. 2015;54(4):581-8. PubMed PMID: 25052542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide. AU - Kang,Ping, AU - Liu,Yulan, AU - Zhu,Huiling, AU - Li,Shuang, AU - Shi,Haifeng, AU - Chen,Feng, AU - Leng,Weibo, AU - Pi,Dinan, AU - Hou,Yongqing, AU - Yi,Dan, Y1 - 2014/07/23/ PY - 2014/05/06/received PY - 2014/07/08/accepted PY - 2014/7/24/entrez PY - 2014/7/24/pubmed PY - 2016/2/26/medline SP - 581 EP - 8 JF - European journal of nutrition JO - Eur J Nutr VL - 54 IS - 4 N2 - PURPOSE: This study was conducted to investigate whether aspartate (Asp) could improve liver energy status in the lipopolysaccharide (LPS)-challenged pigs. METHODS: Twenty-four weaned pigs were assigned to four treatments: (1) nonchallenged control (control diet and saline-treated); (2) LPS-challenged control (the same control diet and LPS-challenged); (3) LPS + 0.5% Asp treatment (0.5% Asp diet and LPS-challenged); and (4) LPS + 1.0% Asp treatment (a 1.0% Asp diet and LPS-challenged). On d 19, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weight, and the same volume of 0.9% NaCl solution, respectively. All pigs were slaughtered at 24 h after LPS or saline injection, and the liver was collected for further analysis. RESULTS: Dietary supplementation with Asp improved liver energy status evidenced by the increased ATP concentration and adenylate energy charges, and the decreased AMP concentration and AMP/ATP ratio (p < 0.05). Asp supplementation increased the mRNA expression of key enzymes in hepatic glycolysis and tricarboxylic acid (TCA) cycle, including pyruvate kinase and citrate synthase (p < 0.05), and had a tendency to increase hepatic pyruvate dehydrogenase and isocitrate dehydrogenase β mRNA expression (p < 0.10). In addition, Asp increased the mRNA expressions of hepatic AMP-activated protein kinase (AMPK) α1, AMPKα2, silent information regulator (Sirt1), and proliferator-activated receptor-γ coactivator 1α (PGC1α) (p < 0.05). Moreover, Asp increased AMPKα phosphorylation (p < 0.05). CONCLUSIONS: These results indicated that dietary supplementation of Asp could improve energy status in LPS-injured liver, which might result from motivating the metabolism pathway of TCA cycle and glycolysis and stimulating the AMPK signaling pathway. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/25052542/The_effect_of_aspartate_on_the_energy_metabolism_in_the_liver_of_weanling_pigs_challenged_with_lipopolysaccharide_ L2 - https://dx.doi.org/10.1007/s00394-014-0739-3 DB - PRIME DP - Unbound Medicine ER -