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Concomitant sensitization to ragweed and mugwort pollen: who is who in clinical allergy?
Ann Allergy Asthma Immunol. 2014 Sep; 113(3):307-13.AA

Abstract

BACKGROUND

In many areas of Europe, double sensitization to ragweed and mugwort is common, and because of the overlapping flowering periods of the 2 plants, it is not possible to diagnose the primary sensitizing allergen source and hence to determine the proper immunotherapy.

OBJECTIVES

To elucidate whether double-sensitized patients are cosensitized or cross-sensitized and, in the latter case, to define the primary sensitizer.

METHODS

Serum samples from 34 patients with late summer respiratory allergy underwent skin prick testing with whole ragweed, and mugwort extracts were analyzed for their reactivity to recombinant Art v 1 and Amb a 1 by ImmunoCAP and then to Amb a 1, Art v 6, and Art v 1 isoforms by a proteomic approach. In double reactors, the primary sensitizing sources were detected by inhibition experiments.

RESULTS

Serum samples from patients monosensitized to ragweed contained IgE to epitopes specific of all Amb a 1 isoforms. In contrast, serum samples from double reactors found to be primarily sensitized to mugwort reacted to Art v 1 and Art v 6 and cross-reacted to a few Amb a 1 isoforms. Finally, serum samples from double reactors found to be primarily sensitized to ragweed contained IgE reacting to all Amb a 1 isoforms, part of which cross-reacted to Art v 6. We did not find cosensitized patients.

CONCLUSION

This study found that Art v 6 plays an important role in mugwort allergy and that the cross-reactivity between Art v 6 and Amb a 1 is frequent, bidirectional, and clinically relevant in the area of Milan.

Authors+Show Affiliations

Ambulatorio di Allergologia, Clinica San Carlo, Paderno Dugnano, Milan, Italy.Dipartimento di Science Ambientali, Università di Milano-Bicocca, Milan, Italy.Dipartimento di Science Ambientali, Università di Milano-Bicocca, Milan, Italy.Dipartimento di Science Ambientali, Università di Milano-Bicocca, Milan, Italy.Allergologia e Immunologia Clinica, Azienda Ospedaliera, S Maria degli Angeli, Pordenone, Italy.Dipartimento di Science Ambientali, Università di Milano-Bicocca, Milan, Italy. Electronic address: sandra.citterio@unimib.it.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25053399

Citation

Asero, Riccardo, et al. "Concomitant Sensitization to Ragweed and Mugwort Pollen: Who Is Who in Clinical Allergy?" Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, vol. 113, no. 3, 2014, pp. 307-13.
Asero R, Bellotto E, Ghiani A, et al. Concomitant sensitization to ragweed and mugwort pollen: who is who in clinical allergy? Ann Allergy Asthma Immunol. 2014;113(3):307-13.
Asero, R., Bellotto, E., Ghiani, A., Aina, R., Villalta, D., & Citterio, S. (2014). Concomitant sensitization to ragweed and mugwort pollen: who is who in clinical allergy? Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, 113(3), 307-13. https://doi.org/10.1016/j.anai.2014.06.009
Asero R, et al. Concomitant Sensitization to Ragweed and Mugwort Pollen: Who Is Who in Clinical Allergy. Ann Allergy Asthma Immunol. 2014;113(3):307-13. PubMed PMID: 25053399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Concomitant sensitization to ragweed and mugwort pollen: who is who in clinical allergy? AU - Asero,Riccardo, AU - Bellotto,Emanuele, AU - Ghiani,Alessandra, AU - Aina,Roberta, AU - Villalta,Danilo, AU - Citterio,Sandra, Y1 - 2014/07/19/ PY - 2014/04/10/received PY - 2014/06/05/revised PY - 2014/06/17/accepted PY - 2014/7/24/entrez PY - 2014/7/24/pubmed PY - 2014/10/29/medline SP - 307 EP - 13 JF - Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology JO - Ann. Allergy Asthma Immunol. VL - 113 IS - 3 N2 - BACKGROUND: In many areas of Europe, double sensitization to ragweed and mugwort is common, and because of the overlapping flowering periods of the 2 plants, it is not possible to diagnose the primary sensitizing allergen source and hence to determine the proper immunotherapy. OBJECTIVES: To elucidate whether double-sensitized patients are cosensitized or cross-sensitized and, in the latter case, to define the primary sensitizer. METHODS: Serum samples from 34 patients with late summer respiratory allergy underwent skin prick testing with whole ragweed, and mugwort extracts were analyzed for their reactivity to recombinant Art v 1 and Amb a 1 by ImmunoCAP and then to Amb a 1, Art v 6, and Art v 1 isoforms by a proteomic approach. In double reactors, the primary sensitizing sources were detected by inhibition experiments. RESULTS: Serum samples from patients monosensitized to ragweed contained IgE to epitopes specific of all Amb a 1 isoforms. In contrast, serum samples from double reactors found to be primarily sensitized to mugwort reacted to Art v 1 and Art v 6 and cross-reacted to a few Amb a 1 isoforms. Finally, serum samples from double reactors found to be primarily sensitized to ragweed contained IgE reacting to all Amb a 1 isoforms, part of which cross-reacted to Art v 6. We did not find cosensitized patients. CONCLUSION: This study found that Art v 6 plays an important role in mugwort allergy and that the cross-reactivity between Art v 6 and Amb a 1 is frequent, bidirectional, and clinically relevant in the area of Milan. SN - 1534-4436 UR - https://www.unboundmedicine.com/medline/citation/25053399/Concomitant_sensitization_to_ragweed_and_mugwort_pollen:_who_is_who_in_clinical_allergy L2 - https://linkinghub.elsevier.com/retrieve/pii/S1081-1206(14)00410-4 DB - PRIME DP - Unbound Medicine ER -