Tags

Type your tag names separated by a space and hit enter

PIK3CA gene mutations and overexpression: implications for prognostic biomarker and therapeutic target in Chinese esophageal squamous cell carcinoma.
PLoS One. 2014; 9(7):e103021.Plos

Abstract

AIMS

To evaluate PIK3CA gene mutations and PIK3CA expression status in Chinese esophageal squamous cell carcinoma (ESCC) patients, and their correlation with clinicopathological characteristics and clinical outcomes.

METHODS

Direct sequencing was applied to investigate mutations in exons 9 and 20 of PIK3CA in 406 Chinese ESCC patients. PIK3CA expression was evaluated using immunohistochemistry analysis. The associations of PIK3CA gene mutations and PIK3CA expression with clinicopathological characteristics and clinical outcome were examined.

RESULTS

Thirty somatic point mutations (30/406, 7.4%) were identified in exon 9 whereas no mutations were detected in exon 20. PIK3CA mutations were not correlated with clinicopathological characteristics or clinical outcomes. However in the ESCC patients with family cancer history, PIK3CA mutations were independently correlated with worse overall survival (multivariate hazard ratio (HR) = 10.493, 95% CI: 2.432-45.267, P = 0.002). Compared to normal esophageal tissue, PIK3CA was significantly overexpressed in cancer tissue (P<0.001). PIK3CA overexpression was independently associated with higher risk of local recurrence (multivariate HR = 1.435, 95% CI: 1.040-1.979, P = 0.028). In female ESCC patients, PIK3CA overexpression was independently correlated with worse overall survival (multivariate HR = 2.341, 95% CI: 1.073-5.108, P = 0.033).

CONCLUSIONS

Our results suggest PIK3CA gene mutation and overexpression could act as biomarkers for individualized molecular targeted therapy for Chinese ESCC patients.

Authors+Show Affiliations

Department of Pathology, Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China.Department of Pathology, Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China.Department of Epidemiology, Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China.Department of Pathology, Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China.Department of Pathology, Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China.Department of Pathology, Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China.Department of Pathology, Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China.Department of Pathology, Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25054828

Citation

Wang, Lin, et al. "PIK3CA Gene Mutations and Overexpression: Implications for Prognostic Biomarker and Therapeutic Target in Chinese Esophageal Squamous Cell Carcinoma." PloS One, vol. 9, no. 7, 2014, pp. e103021.
Wang L, Shan L, Zhang S, et al. PIK3CA gene mutations and overexpression: implications for prognostic biomarker and therapeutic target in Chinese esophageal squamous cell carcinoma. PLoS One. 2014;9(7):e103021.
Wang, L., Shan, L., Zhang, S., Ying, J., Xue, L., Yuan, Y., Xie, Y., & Lu, N. (2014). PIK3CA gene mutations and overexpression: implications for prognostic biomarker and therapeutic target in Chinese esophageal squamous cell carcinoma. PloS One, 9(7), e103021. https://doi.org/10.1371/journal.pone.0103021
Wang L, et al. PIK3CA Gene Mutations and Overexpression: Implications for Prognostic Biomarker and Therapeutic Target in Chinese Esophageal Squamous Cell Carcinoma. PLoS One. 2014;9(7):e103021. PubMed PMID: 25054828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PIK3CA gene mutations and overexpression: implications for prognostic biomarker and therapeutic target in Chinese esophageal squamous cell carcinoma. AU - Wang,Lin, AU - Shan,Ling, AU - Zhang,Shaokai, AU - Ying,Jianming, AU - Xue,Liyan, AU - Yuan,Yanling, AU - Xie,Yongqiang, AU - Lu,Ning, Y1 - 2014/07/23/ PY - 2014/03/29/received PY - 2014/06/24/accepted PY - 2014/7/24/entrez PY - 2014/7/24/pubmed PY - 2015/11/17/medline SP - e103021 EP - e103021 JF - PloS one JO - PLoS One VL - 9 IS - 7 N2 - AIMS: To evaluate PIK3CA gene mutations and PIK3CA expression status in Chinese esophageal squamous cell carcinoma (ESCC) patients, and their correlation with clinicopathological characteristics and clinical outcomes. METHODS: Direct sequencing was applied to investigate mutations in exons 9 and 20 of PIK3CA in 406 Chinese ESCC patients. PIK3CA expression was evaluated using immunohistochemistry analysis. The associations of PIK3CA gene mutations and PIK3CA expression with clinicopathological characteristics and clinical outcome were examined. RESULTS: Thirty somatic point mutations (30/406, 7.4%) were identified in exon 9 whereas no mutations were detected in exon 20. PIK3CA mutations were not correlated with clinicopathological characteristics or clinical outcomes. However in the ESCC patients with family cancer history, PIK3CA mutations were independently correlated with worse overall survival (multivariate hazard ratio (HR) = 10.493, 95% CI: 2.432-45.267, P = 0.002). Compared to normal esophageal tissue, PIK3CA was significantly overexpressed in cancer tissue (P<0.001). PIK3CA overexpression was independently associated with higher risk of local recurrence (multivariate HR = 1.435, 95% CI: 1.040-1.979, P = 0.028). In female ESCC patients, PIK3CA overexpression was independently correlated with worse overall survival (multivariate HR = 2.341, 95% CI: 1.073-5.108, P = 0.033). CONCLUSIONS: Our results suggest PIK3CA gene mutation and overexpression could act as biomarkers for individualized molecular targeted therapy for Chinese ESCC patients. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25054828/PIK3CA_gene_mutations_and_overexpression:_implications_for_prognostic_biomarker_and_therapeutic_target_in_Chinese_esophageal_squamous_cell_carcinoma_ L2 - https://dx.plos.org/10.1371/journal.pone.0103021 DB - PRIME DP - Unbound Medicine ER -