Tags

Type your tag names separated by a space and hit enter

Genotyping, pathogenicity, and immunogenicity of Avibacterium paragallinarum serovar B-1 isolates from the Americas.
Avian Dis 2014; 58(2):293-6AD

Abstract

The bacterium Avibacterium paragallinarum is the etiologic agent of infectious coryza of chickens. Among the nine Kume serovars currently recognized in this bacterium, serovar B-1 is a common serovar in the Americas. In the current study, serovar B-1 isolates from Ecuador (seven isolates), Mexico (seven isolates) and Panama (two isolates) were genotyped. In addition one Panamanian, one Ecuadorian, and two Mexican isolates were used in a vaccination-challenge trial in which the vaccine was based on the 2671 serovar B-1 reference strain. Genotyping by enterobacterial repetitive intergenic consensus-based PCR (ERIC-PCR) resulted in ten distinguishable ERIC patterns for the 16 isolates and the two reference strains of Av. paragallinarum included in the study. No ERIC patterns were shared among isolates of the three different countries. In the vaccination-challenge trial, one isolate from Panama showed a significantly lower virulence than did the three other isolates. In terms of cross-protection, chickens vaccinated with reference strain 2671 and challenged with an Ecuadorian strain showed 40% protection, a significantly lower protection than the homologous protection level. The other three field isolates gave a similar protection level to the homologous challenge.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25055635

Citation

Morales-Erasto, V, et al. "Genotyping, Pathogenicity, and Immunogenicity of Avibacterium Paragallinarum Serovar B-1 Isolates From the Americas." Avian Diseases, vol. 58, no. 2, 2014, pp. 293-6.
Morales-Erasto V, Fernández-Rosas P, Negrete-Abascal E, et al. Genotyping, pathogenicity, and immunogenicity of Avibacterium paragallinarum serovar B-1 isolates from the Americas. Avian Dis. 2014;58(2):293-6.
Morales-Erasto, V., Fernández-Rosas, P., Negrete-Abascal, E., Salazar-García, F., Blackall, P. J., & Soriano-Vargas, E. (2014). Genotyping, pathogenicity, and immunogenicity of Avibacterium paragallinarum serovar B-1 isolates from the Americas. Avian Diseases, 58(2), pp. 293-6.
Morales-Erasto V, et al. Genotyping, Pathogenicity, and Immunogenicity of Avibacterium Paragallinarum Serovar B-1 Isolates From the Americas. Avian Dis. 2014;58(2):293-6. PubMed PMID: 25055635.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotyping, pathogenicity, and immunogenicity of Avibacterium paragallinarum serovar B-1 isolates from the Americas. AU - Morales-Erasto,V, AU - Fernández-Rosas,P, AU - Negrete-Abascal,E, AU - Salazar-García,F, AU - Blackall,P J, AU - Soriano-Vargas,E, PY - 2014/7/25/entrez PY - 2014/7/25/pubmed PY - 2014/8/22/medline SP - 293 EP - 6 JF - Avian diseases JO - Avian Dis. VL - 58 IS - 2 N2 - The bacterium Avibacterium paragallinarum is the etiologic agent of infectious coryza of chickens. Among the nine Kume serovars currently recognized in this bacterium, serovar B-1 is a common serovar in the Americas. In the current study, serovar B-1 isolates from Ecuador (seven isolates), Mexico (seven isolates) and Panama (two isolates) were genotyped. In addition one Panamanian, one Ecuadorian, and two Mexican isolates were used in a vaccination-challenge trial in which the vaccine was based on the 2671 serovar B-1 reference strain. Genotyping by enterobacterial repetitive intergenic consensus-based PCR (ERIC-PCR) resulted in ten distinguishable ERIC patterns for the 16 isolates and the two reference strains of Av. paragallinarum included in the study. No ERIC patterns were shared among isolates of the three different countries. In the vaccination-challenge trial, one isolate from Panama showed a significantly lower virulence than did the three other isolates. In terms of cross-protection, chickens vaccinated with reference strain 2671 and challenged with an Ecuadorian strain showed 40% protection, a significantly lower protection than the homologous protection level. The other three field isolates gave a similar protection level to the homologous challenge. SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/25055635/Genotyping_pathogenicity_and_immunogenicity_of_Avibacterium_paragallinarum_serovar_B_1_isolates_from_the_Americas_ L2 - http://www.bioone.org/doi/10.1637/10693-101513-ResNote.1?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -