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Volumetric absorptive microsampling: a dried sample collection technique for quantitative bioanalysis.
Anal Chem. 2014 Aug 19; 86(16):8489-95.AC

Abstract

Volumetric absorptive microsampling (VAMS) is a novel approach to obtaining a dried blood sample for quantitative bioanalysis that overcomes the area bias and homogeneity issues associated with conventional dried blood spot (DBS) sample when a subpunch is taken. The VAMS sampler absorbs a fixed volume of blood (∼10 μL) in 2-4 s with less than 5% volume variation across the hematocrit range of 20-70% with low tip-to-tip variability. There is no evidence of selective absorption by the tip of the plasma component over whole blood. Recommendations for best practice when collecting samples were developed based upon the results of tests examining a number of potential abuse scenarios.

Authors+Show Affiliations

Bioanalytical Science and Toxicokinetics, Drug Metabolism and Pharmacokinetics, GlaxoSmithKline Research and Development , Ware, Hertfordshire SG12 0DP, United Kingdom.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25058158

Citation

Denniff, Philip, and Neil Spooner. "Volumetric Absorptive Microsampling: a Dried Sample Collection Technique for Quantitative Bioanalysis." Analytical Chemistry, vol. 86, no. 16, 2014, pp. 8489-95.
Denniff P, Spooner N. Volumetric absorptive microsampling: a dried sample collection technique for quantitative bioanalysis. Anal Chem. 2014;86(16):8489-95.
Denniff, P., & Spooner, N. (2014). Volumetric absorptive microsampling: a dried sample collection technique for quantitative bioanalysis. Analytical Chemistry, 86(16), 8489-95. https://doi.org/10.1021/ac5022562
Denniff P, Spooner N. Volumetric Absorptive Microsampling: a Dried Sample Collection Technique for Quantitative Bioanalysis. Anal Chem. 2014 Aug 19;86(16):8489-95. PubMed PMID: 25058158.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Volumetric absorptive microsampling: a dried sample collection technique for quantitative bioanalysis. AU - Denniff,Philip, AU - Spooner,Neil, Y1 - 2014/08/06/ PY - 2014/7/25/entrez PY - 2014/7/25/pubmed PY - 2015/8/20/medline SP - 8489 EP - 95 JF - Analytical chemistry JO - Anal. Chem. VL - 86 IS - 16 N2 - Volumetric absorptive microsampling (VAMS) is a novel approach to obtaining a dried blood sample for quantitative bioanalysis that overcomes the area bias and homogeneity issues associated with conventional dried blood spot (DBS) sample when a subpunch is taken. The VAMS sampler absorbs a fixed volume of blood (∼10 μL) in 2-4 s with less than 5% volume variation across the hematocrit range of 20-70% with low tip-to-tip variability. There is no evidence of selective absorption by the tip of the plasma component over whole blood. Recommendations for best practice when collecting samples were developed based upon the results of tests examining a number of potential abuse scenarios. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/25058158/Volumetric_absorptive_microsampling:_a_dried_sample_collection_technique_for_quantitative_bioanalysis_ L2 - https://dx.doi.org/10.1021/ac5022562 DB - PRIME DP - Unbound Medicine ER -