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Complete mitochondrial genomes of five skippers (Lepidoptera: Hesperiidae) and phylogenetic reconstruction of Lepidoptera.
Gene. 2014 Oct 01; 549(1):97-112.GENE

Abstract

We sequenced mitogenomes of five skippers (family Hesperiidae, Lepidoptera) to obtain further insight into the characteristics of butterfly mitogenomes and performed phylogenetic reconstruction using all available gene sequences (PCGs, rRNAs, and tRNAs) from 85 species (20 families in eight superfamilies). The general genomic features found in the butterflies also were found in the five skippers: a high A+T composition (79.3%-80.9%), dominant usage of TAA stop codon, similar skewness pattern in both strands, consistently length intergenic spacer sequence between tRNA(Gln) and ND2 (64-87 bp), conserved ATACTAA motif between tRNA(Ser (UCN)) and ND1, and characteristic features of the A+T-rich region (the ATAGA motif, varying length of poly-T stretch, and poly-A stretch). The start codon for COI was CGA in four skippers as typical, but Lobocla bifasciatus evidently possessed canonical ATG as start codon. All species had the ancestral arrangement tRNA(Asn)/tRNA(Ser (AGN)), instead of the rearrangement tRNA(Ser (AGN))/tRNA(Asn), found in another skipper species (Erynnis). Phylogenetic analyses using all available genes (PCGs, rRNAS, and tRNAs) yielded the consensus superfamilial relationships ((((((Bombycoidea+Noctuoidea+Geometroidea)+Pyraloidea)+Papilionoidea)+Tortricoidea)+Yponomeutoidea)+Hepialoidea), confirming the validity of Macroheterocera (Bombycoidea, Noctuoidea, and Geometroidea in this study) and its sister relationship to Pyraloidea. Within Rhopalocera (butterflies and skippers) the familial relationships (Papilionidae+(Hesperiidae+(Pieridae+((Lycaenidae+Riodinidae)+Nymphalidae)))) were strongly supported in all analyses (0.98-1 by BI and 96-100 by ML methods), rendering invalid the superfamily status for Hesperioidea. On the other hand, current mitogenome-based phylogeny did not find consistent superfamilial relationships among Noctuoidea, Geometroidea, and Bombycoidea and the familial relationships within Bombycoidea between analyses, requiring further taxon sampling in future studies.

Authors+Show Affiliations

College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: ikkim81@chonnam.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25058696

Citation

Kim, Min Jee, et al. "Complete Mitochondrial Genomes of Five Skippers (Lepidoptera: Hesperiidae) and Phylogenetic Reconstruction of Lepidoptera." Gene, vol. 549, no. 1, 2014, pp. 97-112.
Kim MJ, Wang AR, Park JS, et al. Complete mitochondrial genomes of five skippers (Lepidoptera: Hesperiidae) and phylogenetic reconstruction of Lepidoptera. Gene. 2014;549(1):97-112.
Kim, M. J., Wang, A. R., Park, J. S., & Kim, I. (2014). Complete mitochondrial genomes of five skippers (Lepidoptera: Hesperiidae) and phylogenetic reconstruction of Lepidoptera. Gene, 549(1), 97-112. https://doi.org/10.1016/j.gene.2014.07.052
Kim MJ, et al. Complete Mitochondrial Genomes of Five Skippers (Lepidoptera: Hesperiidae) and Phylogenetic Reconstruction of Lepidoptera. Gene. 2014 Oct 1;549(1):97-112. PubMed PMID: 25058696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complete mitochondrial genomes of five skippers (Lepidoptera: Hesperiidae) and phylogenetic reconstruction of Lepidoptera. AU - Kim,Min Jee, AU - Wang,Ah Rha, AU - Park,Jeong Sun, AU - Kim,Iksoo, Y1 - 2014/07/22/ PY - 2014/04/02/received PY - 2014/07/02/revised PY - 2014/07/18/accepted PY - 2014/7/25/entrez PY - 2014/7/25/pubmed PY - 2014/10/22/medline KW - Hesperiidae KW - Lepidopteran phylogeny KW - Mitochondrial DNA KW - Mitogenome KW - Papilionoidea SP - 97 EP - 112 JF - Gene JO - Gene VL - 549 IS - 1 N2 - We sequenced mitogenomes of five skippers (family Hesperiidae, Lepidoptera) to obtain further insight into the characteristics of butterfly mitogenomes and performed phylogenetic reconstruction using all available gene sequences (PCGs, rRNAs, and tRNAs) from 85 species (20 families in eight superfamilies). The general genomic features found in the butterflies also were found in the five skippers: a high A+T composition (79.3%-80.9%), dominant usage of TAA stop codon, similar skewness pattern in both strands, consistently length intergenic spacer sequence between tRNA(Gln) and ND2 (64-87 bp), conserved ATACTAA motif between tRNA(Ser (UCN)) and ND1, and characteristic features of the A+T-rich region (the ATAGA motif, varying length of poly-T stretch, and poly-A stretch). The start codon for COI was CGA in four skippers as typical, but Lobocla bifasciatus evidently possessed canonical ATG as start codon. All species had the ancestral arrangement tRNA(Asn)/tRNA(Ser (AGN)), instead of the rearrangement tRNA(Ser (AGN))/tRNA(Asn), found in another skipper species (Erynnis). Phylogenetic analyses using all available genes (PCGs, rRNAS, and tRNAs) yielded the consensus superfamilial relationships ((((((Bombycoidea+Noctuoidea+Geometroidea)+Pyraloidea)+Papilionoidea)+Tortricoidea)+Yponomeutoidea)+Hepialoidea), confirming the validity of Macroheterocera (Bombycoidea, Noctuoidea, and Geometroidea in this study) and its sister relationship to Pyraloidea. Within Rhopalocera (butterflies and skippers) the familial relationships (Papilionidae+(Hesperiidae+(Pieridae+((Lycaenidae+Riodinidae)+Nymphalidae)))) were strongly supported in all analyses (0.98-1 by BI and 96-100 by ML methods), rendering invalid the superfamily status for Hesperioidea. On the other hand, current mitogenome-based phylogeny did not find consistent superfamilial relationships among Noctuoidea, Geometroidea, and Bombycoidea and the familial relationships within Bombycoidea between analyses, requiring further taxon sampling in future studies. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/25058696/Complete_mitochondrial_genomes_of_five_skippers__Lepidoptera:_Hesperiidae__and_phylogenetic_reconstruction_of_Lepidoptera_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(14)00856-7 DB - PRIME DP - Unbound Medicine ER -