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Proanthocyanidins in wild sea buckthorn (Hippophaë rhamnoides) berries analyzed by reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography with UV and MS detection.
J Agric Food Chem. 2014 Aug 06; 62(31):7721-9.JA

Abstract

A rapid and sensitive method for profiling of proanthocyanidins (PAs) of sea buckthorn (Hippophaë rhamnoides) berries was established based on aqueous, acidified acetone extraction. The extract was purified by Sephadex column chromatography and analyzed using reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography (HILIC). Negative ion electrospray ionization mass spectrometry (ESI-MS) in single ion recording (SIR) and full scan modes combined with UV detection were used to define the combinations and ratios of PA oligomer classes. PAs with degree of polymerization from 2 to 11 were detected by HILIC-ESI-MS. Quantification of dimeric, trimeric, and tetrameric PAs was carried out with ESI-MS-SIR, and their molar proportions were 40, 40, and 20%, respectively. Only B-type PAs were found, and (epi)gallocatechins were the main monomeric units. More than 60 combinations of (epi)catechins and (epi)gallocatechins of proanthocyanidin dimers and trimers were found. A majority of the PAs were shown to be higher polymers based on the HILIC-UV analysis.

Authors+Show Affiliations

Food Chemistry and Food Development, Department of Biochemistry, University of Turku , FI-20014, Turku, Finland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25061802

Citation

Kallio, Heikki, et al. "Proanthocyanidins in Wild Sea Buckthorn (Hippophaë Rhamnoides) Berries Analyzed By Reversed-phase, Normal-phase, and Hydrophilic Interaction Liquid Chromatography With UV and MS Detection." Journal of Agricultural and Food Chemistry, vol. 62, no. 31, 2014, pp. 7721-9.
Kallio H, Yang W, Liu P, et al. Proanthocyanidins in wild sea buckthorn (Hippophaë rhamnoides) berries analyzed by reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography with UV and MS detection. J Agric Food Chem. 2014;62(31):7721-9.
Kallio, H., Yang, W., Liu, P., & Yang, B. (2014). Proanthocyanidins in wild sea buckthorn (Hippophaë rhamnoides) berries analyzed by reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography with UV and MS detection. Journal of Agricultural and Food Chemistry, 62(31), 7721-9. https://doi.org/10.1021/jf502056f
Kallio H, et al. Proanthocyanidins in Wild Sea Buckthorn (Hippophaë Rhamnoides) Berries Analyzed By Reversed-phase, Normal-phase, and Hydrophilic Interaction Liquid Chromatography With UV and MS Detection. J Agric Food Chem. 2014 Aug 6;62(31):7721-9. PubMed PMID: 25061802.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proanthocyanidins in wild sea buckthorn (Hippophaë rhamnoides) berries analyzed by reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography with UV and MS detection. AU - Kallio,Heikki, AU - Yang,Wei, AU - Liu,Pengzhan, AU - Yang,Baoru, Y1 - 2014/07/25/ PY - 2014/7/26/entrez PY - 2014/7/26/pubmed PY - 2015/4/14/medline SP - 7721 EP - 9 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 62 IS - 31 N2 - A rapid and sensitive method for profiling of proanthocyanidins (PAs) of sea buckthorn (Hippophaë rhamnoides) berries was established based on aqueous, acidified acetone extraction. The extract was purified by Sephadex column chromatography and analyzed using reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography (HILIC). Negative ion electrospray ionization mass spectrometry (ESI-MS) in single ion recording (SIR) and full scan modes combined with UV detection were used to define the combinations and ratios of PA oligomer classes. PAs with degree of polymerization from 2 to 11 were detected by HILIC-ESI-MS. Quantification of dimeric, trimeric, and tetrameric PAs was carried out with ESI-MS-SIR, and their molar proportions were 40, 40, and 20%, respectively. Only B-type PAs were found, and (epi)gallocatechins were the main monomeric units. More than 60 combinations of (epi)catechins and (epi)gallocatechins of proanthocyanidin dimers and trimers were found. A majority of the PAs were shown to be higher polymers based on the HILIC-UV analysis. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/25061802/Proanthocyanidins_in_wild_sea_buckthorn__Hippophaë_rhamnoides__berries_analyzed_by_reversed_phase_normal_phase_and_hydrophilic_interaction_liquid_chromatography_with_UV_and_MS_detection_ L2 - https://doi.org/10.1021/jf502056f DB - PRIME DP - Unbound Medicine ER -