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Cranberry extract standardized for proanthocyanidins promotes the immune response of Caenorhabditis elegans to Vibrio cholerae through the p38 MAPK pathway and HSF-1.
PLoS One. 2014; 9(7):e103290.Plos

Abstract

Botanicals are rich in bioactive compounds, and some offer numerous beneficial effects to animal and human health when consumed. It is well known that phytochemicals in cranberries have anti-oxidative and antimicrobial activities. Recently, an increasing body of evidence has demonstrated that cranberry phytochemicals may have potential benefits that promote healthy aging. Here, we use Caenorhabditis elegans as a model to show that water-soluble cranberry extract standardized to 4.0% proanthocyanidins (WCESP), a major component of cranberries, can enhance host innate immunity to resist against Vibrio cholerae (V. cholerae; wild type C6706 (O1 El Tor biotype)) infection. Supplementation of WCESP did not significantly alter the intestinal colonization of V. cholerae, but upregulated the expression of C. elegans innate immune genes, such as clec-46, clec-71, fmo-2, pqn-5 and C23G10.1. Additionally, WCESP treatment did not affect the growth of V. cholerae and expression of the major bacterial virulence genes, and only slightly reduced bacterial colonization within C. elegans intestine. These findings indicate that the major components of WCESP, including proanthocyanidins (PACs), may play an important role in enhancing the host innate immunity. Moreover, we engaged C. elegans mutants and identified that the p38 MAPK signaling, insulin/IGF-1 signaling (IIS), and HSF-1 play pivotal roles in the WCESP-mediated host immune response. Considering the level of conservation between the innate immune pathways of C. elegans and humans, the results of this study suggest that WCESP may also play an immunity-promoting role in higher order organisms.

Authors+Show Affiliations

Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America.Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America.Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America.Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America.Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America; Institute for Engaged Aging, Clemson University, Clemson, South Carolina, United States of America.Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America; Institute for Engaged Aging, Clemson University, Clemson, South Carolina, United States of America.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25062095

Citation

Dinh, Jessica, et al. "Cranberry Extract Standardized for Proanthocyanidins Promotes the Immune Response of Caenorhabditis Elegans to Vibrio Cholerae Through the P38 MAPK Pathway and HSF-1." PloS One, vol. 9, no. 7, 2014, pp. e103290.
Dinh J, Angeloni JT, Pederson DB, et al. Cranberry extract standardized for proanthocyanidins promotes the immune response of Caenorhabditis elegans to Vibrio cholerae through the p38 MAPK pathway and HSF-1. PLoS One. 2014;9(7):e103290.
Dinh, J., Angeloni, J. T., Pederson, D. B., Wang, X., Cao, M., & Dong, Y. (2014). Cranberry extract standardized for proanthocyanidins promotes the immune response of Caenorhabditis elegans to Vibrio cholerae through the p38 MAPK pathway and HSF-1. PloS One, 9(7), e103290. https://doi.org/10.1371/journal.pone.0103290
Dinh J, et al. Cranberry Extract Standardized for Proanthocyanidins Promotes the Immune Response of Caenorhabditis Elegans to Vibrio Cholerae Through the P38 MAPK Pathway and HSF-1. PLoS One. 2014;9(7):e103290. PubMed PMID: 25062095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cranberry extract standardized for proanthocyanidins promotes the immune response of Caenorhabditis elegans to Vibrio cholerae through the p38 MAPK pathway and HSF-1. AU - Dinh,Jessica, AU - Angeloni,Joseph T, AU - Pederson,Daniel B, AU - Wang,Xiaoxia, AU - Cao,Min, AU - Dong,Yuqing, Y1 - 2014/07/25/ PY - 2014/03/12/received PY - 2014/06/27/accepted PY - 2014/7/26/entrez PY - 2014/7/26/pubmed PY - 2015/4/17/medline SP - e103290 EP - e103290 JF - PloS one JO - PLoS One VL - 9 IS - 7 N2 - Botanicals are rich in bioactive compounds, and some offer numerous beneficial effects to animal and human health when consumed. It is well known that phytochemicals in cranberries have anti-oxidative and antimicrobial activities. Recently, an increasing body of evidence has demonstrated that cranberry phytochemicals may have potential benefits that promote healthy aging. Here, we use Caenorhabditis elegans as a model to show that water-soluble cranberry extract standardized to 4.0% proanthocyanidins (WCESP), a major component of cranberries, can enhance host innate immunity to resist against Vibrio cholerae (V. cholerae; wild type C6706 (O1 El Tor biotype)) infection. Supplementation of WCESP did not significantly alter the intestinal colonization of V. cholerae, but upregulated the expression of C. elegans innate immune genes, such as clec-46, clec-71, fmo-2, pqn-5 and C23G10.1. Additionally, WCESP treatment did not affect the growth of V. cholerae and expression of the major bacterial virulence genes, and only slightly reduced bacterial colonization within C. elegans intestine. These findings indicate that the major components of WCESP, including proanthocyanidins (PACs), may play an important role in enhancing the host innate immunity. Moreover, we engaged C. elegans mutants and identified that the p38 MAPK signaling, insulin/IGF-1 signaling (IIS), and HSF-1 play pivotal roles in the WCESP-mediated host immune response. Considering the level of conservation between the innate immune pathways of C. elegans and humans, the results of this study suggest that WCESP may also play an immunity-promoting role in higher order organisms. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25062095/Cranberry_extract_standardized_for_proanthocyanidins_promotes_the_immune_response_of_Caenorhabditis_elegans_to_Vibrio_cholerae_through_the_p38_MAPK_pathway_and_HSF_1_ L2 - https://dx.plos.org/10.1371/journal.pone.0103290 DB - PRIME DP - Unbound Medicine ER -