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Preliminary Investigations on the Distribution of Leptospira Serovars in Domestic Animals in North-west Morocco.
Transbound Emerg Dis 2016; 63(2):e178-84TE

Abstract

Leptospirosis is a neglected zoonosis of global importance with a complex epidemiology that affects humans, domestic and wild mammals. However, due to the diversity of clinical signs and difficulties of establishing a confirmatory laboratory diagnosis, the disease remains poorly investigated, particularly in the developing world. In Morocco, a descriptive study of the seroprevalence of Leptospira infection in animals has never been undertaken. To fill this gap, the current study was conducted on a subset of animals in north-west Morocco as a preliminary step towards understanding the epidemiological patterns of animal leptospirosis in the country. The study was conducted on 289 serum samples collected between January and April 2012 from dogs, cattle, sheep, goats and donkeys in the areas of Rabat-Temara, Sidi Kacem and Oulmes. All serum samples were tested by the MAT with 14 reference strains of the most prevalent pathogenic serovars of Leptospira and two serovars of non-pathogenic Leptospira. The overall seroprevalence of Leptospira in cattle, sheep, goats, dogs and donkeys was 15%, 18%, 20%, 21% and 20%, respectively. The most prevalent serogroups found in each species were Ballum, Sejroe, and Australis in cattle, Ballum, Australis and Sejroe in sheep, Australis and Ballum in goats, Javanica and Australis in donkey and Australis, Ballum and Canicola in dogs. Of all the serogroups tested in this study, Icterohaemorrhagiae, the only serogroup which has been previously reported in humans in Morocco, was rarely reactive. The majority of reactive sera were collected from low land areas. A large number of sera samples classified as seronegative when tested against pathogenic leptospires were positive when tested against non-pathogenic leptospires; this is suggestive of possible novel, as yet unclassified, Leptospira serovars in Morocco. Eleven of thirteen sheep urine samples were positive by real-time PCR confirming their role as Leptospira carriers in Morocco.

Authors+Show Affiliations

Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco.Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco.KIT Biomedical Research, Amsterdam, The Netherlands.KIT Biomedical Research, Amsterdam, The Netherlands.KIT Biomedical Research, Amsterdam, The Netherlands.School of Veterinary Medicine, University College Dublin, Dublin, Ireland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25065690

Citation

Benkirane, A, et al. "Preliminary Investigations On the Distribution of Leptospira Serovars in Domestic Animals in North-west Morocco." Transboundary and Emerging Diseases, vol. 63, no. 2, 2016, pp. e178-84.
Benkirane A, Noury S, Hartskeerl RA, et al. Preliminary Investigations on the Distribution of Leptospira Serovars in Domestic Animals in North-west Morocco. Transbound Emerg Dis. 2016;63(2):e178-84.
Benkirane, A., Noury, S., Hartskeerl, R. A., Goris, M. G., Ahmed, A., & Nally, J. E. (2016). Preliminary Investigations on the Distribution of Leptospira Serovars in Domestic Animals in North-west Morocco. Transboundary and Emerging Diseases, 63(2), pp. e178-84. doi:10.1111/tbed.12252.
Benkirane A, et al. Preliminary Investigations On the Distribution of Leptospira Serovars in Domestic Animals in North-west Morocco. Transbound Emerg Dis. 2016;63(2):e178-84. PubMed PMID: 25065690.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preliminary Investigations on the Distribution of Leptospira Serovars in Domestic Animals in North-west Morocco. AU - Benkirane,A, AU - Noury,S, AU - Hartskeerl,R A, AU - Goris,M G A, AU - Ahmed,A, AU - Nally,J E, Y1 - 2014/07/26/ PY - 2014/02/21/received PY - 2014/7/29/entrez PY - 2014/7/30/pubmed PY - 2016/11/9/medline KW - Leptospira KW - Morocco KW - domestic animals KW - leptospirosis KW - zoonosis SP - e178 EP - 84 JF - Transboundary and emerging diseases JO - Transbound Emerg Dis VL - 63 IS - 2 N2 - Leptospirosis is a neglected zoonosis of global importance with a complex epidemiology that affects humans, domestic and wild mammals. However, due to the diversity of clinical signs and difficulties of establishing a confirmatory laboratory diagnosis, the disease remains poorly investigated, particularly in the developing world. In Morocco, a descriptive study of the seroprevalence of Leptospira infection in animals has never been undertaken. To fill this gap, the current study was conducted on a subset of animals in north-west Morocco as a preliminary step towards understanding the epidemiological patterns of animal leptospirosis in the country. The study was conducted on 289 serum samples collected between January and April 2012 from dogs, cattle, sheep, goats and donkeys in the areas of Rabat-Temara, Sidi Kacem and Oulmes. All serum samples were tested by the MAT with 14 reference strains of the most prevalent pathogenic serovars of Leptospira and two serovars of non-pathogenic Leptospira. The overall seroprevalence of Leptospira in cattle, sheep, goats, dogs and donkeys was 15%, 18%, 20%, 21% and 20%, respectively. The most prevalent serogroups found in each species were Ballum, Sejroe, and Australis in cattle, Ballum, Australis and Sejroe in sheep, Australis and Ballum in goats, Javanica and Australis in donkey and Australis, Ballum and Canicola in dogs. Of all the serogroups tested in this study, Icterohaemorrhagiae, the only serogroup which has been previously reported in humans in Morocco, was rarely reactive. The majority of reactive sera were collected from low land areas. A large number of sera samples classified as seronegative when tested against pathogenic leptospires were positive when tested against non-pathogenic leptospires; this is suggestive of possible novel, as yet unclassified, Leptospira serovars in Morocco. Eleven of thirteen sheep urine samples were positive by real-time PCR confirming their role as Leptospira carriers in Morocco. SN - 1865-1682 UR - https://www.unboundmedicine.com/medline/citation/25065690/Preliminary_Investigations_on_the_Distribution_of_Leptospira_Serovars_in_Domestic_Animals_in_North_west_Morocco_ L2 - https://doi.org/10.1111/tbed.12252 DB - PRIME DP - Unbound Medicine ER -