Tags

Type your tag names separated by a space and hit enter

Morin hydrate augments phagocytosis mechanism and inhibits LPS induced autophagic signaling in murine macrophage.
Int Immunopharmacol. 2014 Oct; 22(2):356-65.II

Abstract

Morin, a natural flavonoid that is the primary bioactive constituent of the family Moraceae, has been found to be associated with many therapeutic properties. In this study, we evaluated the immunomodulatory activities of increasing concentration of morin hydrate in vitro. Three different concentrations of morin hydrate (5, 10, and 15μM) were used to evaluate their effect on splenocyte proliferation, phagocytic activity of macrophages, cytokine secretion and complement inhibition. We also evaluated the role of morin hydrate on lipopolysaccharide (LPS) induced autophagy. Our study demonstrated that morin hydrate elicited a significant increase in splenocyte proliferation, phagocytic capacity and suppressed the production of cytokines and nitric oxide in activated macrophages. Humoral immunity measured by anti-complement activity showed an increase in inhibition of the complement system after the addition of morin hydrate, where morin hydrate at 15μM concentration induced a significant inhibition. Depending on our results, we can also conclude that morin hydrate protects macrophages from LPS induced autophagic cell death. Our findings suggest that morin hydrate represents a structurally diverse class of flavonoid and this structural variability can profoundly affect its cell-type specificity and its biological activities. Supplementation of immune cells with morin hydrate has an upregulating and immunoprotective effect that shows potential as a countermeasure to the immune dysfunction and suggests an interesting use in inflammation related diseases.

Authors+Show Affiliations

Department of Biotechnology, Daegu University, Kyoungsan, Kyoungbook 712-714, Republic of Korea.Department of Biotechnology, Daegu University, Kyoungsan, Kyoungbook 712-714, Republic of Korea.Department of Biotechnology, Daegu University, Kyoungsan, Kyoungbook 712-714, Republic of Korea.Department of Biotechnology, Daegu University, Kyoungsan, Kyoungbook 712-714, Republic of Korea. Electronic address: sckang@daegu.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25068824

Citation

Jakhar, Rekha, et al. "Morin Hydrate Augments Phagocytosis Mechanism and Inhibits LPS Induced Autophagic Signaling in Murine Macrophage." International Immunopharmacology, vol. 22, no. 2, 2014, pp. 356-65.
Jakhar R, Paul S, Chauhan AK, et al. Morin hydrate augments phagocytosis mechanism and inhibits LPS induced autophagic signaling in murine macrophage. Int Immunopharmacol. 2014;22(2):356-65.
Jakhar, R., Paul, S., Chauhan, A. K., & Kang, S. C. (2014). Morin hydrate augments phagocytosis mechanism and inhibits LPS induced autophagic signaling in murine macrophage. International Immunopharmacology, 22(2), 356-65. https://doi.org/10.1016/j.intimp.2014.07.020
Jakhar R, et al. Morin Hydrate Augments Phagocytosis Mechanism and Inhibits LPS Induced Autophagic Signaling in Murine Macrophage. Int Immunopharmacol. 2014;22(2):356-65. PubMed PMID: 25068824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morin hydrate augments phagocytosis mechanism and inhibits LPS induced autophagic signaling in murine macrophage. AU - Jakhar,Rekha, AU - Paul,Souren, AU - Chauhan,Anil Kumar, AU - Kang,Sun Chul, Y1 - 2014/07/26/ PY - 2014/05/23/received PY - 2014/07/01/revised PY - 2014/07/16/accepted PY - 2014/7/29/entrez PY - 2014/7/30/pubmed PY - 2015/6/10/medline KW - Anticomplement KW - Autophagy KW - Cytokine KW - Immunomodulation KW - Nitric oxide KW - Phagocytosis SP - 356 EP - 65 JF - International immunopharmacology JO - Int Immunopharmacol VL - 22 IS - 2 N2 - Morin, a natural flavonoid that is the primary bioactive constituent of the family Moraceae, has been found to be associated with many therapeutic properties. In this study, we evaluated the immunomodulatory activities of increasing concentration of morin hydrate in vitro. Three different concentrations of morin hydrate (5, 10, and 15μM) were used to evaluate their effect on splenocyte proliferation, phagocytic activity of macrophages, cytokine secretion and complement inhibition. We also evaluated the role of morin hydrate on lipopolysaccharide (LPS) induced autophagy. Our study demonstrated that morin hydrate elicited a significant increase in splenocyte proliferation, phagocytic capacity and suppressed the production of cytokines and nitric oxide in activated macrophages. Humoral immunity measured by anti-complement activity showed an increase in inhibition of the complement system after the addition of morin hydrate, where morin hydrate at 15μM concentration induced a significant inhibition. Depending on our results, we can also conclude that morin hydrate protects macrophages from LPS induced autophagic cell death. Our findings suggest that morin hydrate represents a structurally diverse class of flavonoid and this structural variability can profoundly affect its cell-type specificity and its biological activities. Supplementation of immune cells with morin hydrate has an upregulating and immunoprotective effect that shows potential as a countermeasure to the immune dysfunction and suggests an interesting use in inflammation related diseases. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/25068824/Morin_hydrate_augments_phagocytosis_mechanism_and_inhibits_LPS_induced_autophagic_signaling_in_murine_macrophage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(14)00285-9 DB - PRIME DP - Unbound Medicine ER -