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Transcriptome analysis reveals the same 17 S-locus F-box genes in two haplotypes of the self-incompatibility locus of Petunia inflata.
Plant Cell. 2014 Jul; 26(7):2873-88.PC

Abstract

Petunia possesses self-incompatibility, by which pistils reject self-pollen but accept non-self-pollen for fertilization. Self-/non-self-recognition between pollen and pistil is regulated by the pistil-specific S-RNase gene and by multiple pollen-specific S-locus F-box (SLF) genes. To date, 10 SLF genes have been identified by various methods, and seven have been shown to be involved in pollen specificity. For a given S-haplotype, each SLF interacts with a subset of its non-self S-RNases, and an as yet unknown number of SLFs are thought to collectively mediate ubiquitination and degradation of all non-self S-RNases to allow cross-compatible pollination. To identify a complete suite of SLF genes of P. inflata, we used a de novo RNA-seq approach to analyze the pollen transcriptomes of S2-haplotype and S3-haplotype, as well as the leaf transcriptome of the S3S3 genotype. We searched for genes that fit several criteria established from the properties of the known SLF genes and identified the same seven new SLF genes in S2-haplotype and S3-haplotype, suggesting that a total of 17 SLF genes constitute pollen specificity in each S-haplotype. This finding lays the foundation for understanding how multiple SLF genes evolved and the biochemical basis for differential interactions between SLF proteins and S-RNases.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802 Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802.Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802 txk3@psu.edu.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25070642

Citation

Williams, Justin S., et al. "Transcriptome Analysis Reveals the Same 17 S-locus F-box Genes in Two Haplotypes of the Self-incompatibility Locus of Petunia Inflata." The Plant Cell, vol. 26, no. 7, 2014, pp. 2873-88.
Williams JS, Der JP, dePamphilis CW, et al. Transcriptome analysis reveals the same 17 S-locus F-box genes in two haplotypes of the self-incompatibility locus of Petunia inflata. Plant Cell. 2014;26(7):2873-88.
Williams, J. S., Der, J. P., dePamphilis, C. W., & Kao, T. H. (2014). Transcriptome analysis reveals the same 17 S-locus F-box genes in two haplotypes of the self-incompatibility locus of Petunia inflata. The Plant Cell, 26(7), 2873-88. https://doi.org/10.1105/tpc.114.126920
Williams JS, et al. Transcriptome Analysis Reveals the Same 17 S-locus F-box Genes in Two Haplotypes of the Self-incompatibility Locus of Petunia Inflata. Plant Cell. 2014;26(7):2873-88. PubMed PMID: 25070642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome analysis reveals the same 17 S-locus F-box genes in two haplotypes of the self-incompatibility locus of Petunia inflata. AU - Williams,Justin S, AU - Der,Joshua P, AU - dePamphilis,Claude W, AU - Kao,Teh-Hui, Y1 - 2014/07/28/ PY - 2014/7/30/entrez PY - 2014/7/30/pubmed PY - 2015/7/15/medline SP - 2873 EP - 88 JF - The Plant cell JO - Plant Cell VL - 26 IS - 7 N2 - Petunia possesses self-incompatibility, by which pistils reject self-pollen but accept non-self-pollen for fertilization. Self-/non-self-recognition between pollen and pistil is regulated by the pistil-specific S-RNase gene and by multiple pollen-specific S-locus F-box (SLF) genes. To date, 10 SLF genes have been identified by various methods, and seven have been shown to be involved in pollen specificity. For a given S-haplotype, each SLF interacts with a subset of its non-self S-RNases, and an as yet unknown number of SLFs are thought to collectively mediate ubiquitination and degradation of all non-self S-RNases to allow cross-compatible pollination. To identify a complete suite of SLF genes of P. inflata, we used a de novo RNA-seq approach to analyze the pollen transcriptomes of S2-haplotype and S3-haplotype, as well as the leaf transcriptome of the S3S3 genotype. We searched for genes that fit several criteria established from the properties of the known SLF genes and identified the same seven new SLF genes in S2-haplotype and S3-haplotype, suggesting that a total of 17 SLF genes constitute pollen specificity in each S-haplotype. This finding lays the foundation for understanding how multiple SLF genes evolved and the biochemical basis for differential interactions between SLF proteins and S-RNases. SN - 1532-298X UR - https://www.unboundmedicine.com/medline/citation/25070642/Transcriptome_analysis_reveals_the_same_17_S_locus_F_box_genes_in_two_haplotypes_of_the_self_incompatibility_locus_of_Petunia_inflata_ DB - PRIME DP - Unbound Medicine ER -