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Effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (Myzus persicae).
Pest Manag Sci. 2015 Jul; 71(7):951-6.PM

Abstract

BACKGROUND

The recombinant fusion proteins Pl1a/GNA and Hv1a/GNA contain the spider venom peptides δ-amaurobitoxin-PI1a or ω-hexatoxin-Hv1a respectively, linked to snowdrop lectin (GNA). Pl1a targets receptor site 4 of insect voltage-gated sodium channels (NaCh), while Hv1a targets voltage-gated calcium channels. Insecticide-resistant strains of peach-potato aphid (Myzus persicae) contain mutations in NaCh. The pyrethroid-resistant kdr (794J) and super-kdr (UKO) strains contain mutations at residues L1014 and M918 in the channel α-subunit respectively, while the kdr + super-kdr strain (4824J), insensitive to pyrethroids, contains mutations at both L1014 and M918.

RESULTS

Pl1a/GNA and Hv1a/GNA fusion proteins have estimated LC50 values of 0.35 and 0.19 mg mL(-1) when fed to wild-type M. persicae. For insecticide-resistant aphids, LC50 for the Pl1a/GNA fusion protein increased by 2-6-fold, correlating with pyrethroid resistance (wild type < kdr < super-kdr < kdr + super-kdr strains). In contrast, LC50 for the Hv1a/GNA fusion protein showed limited correlation with pyrethroid resistance.

CONCLUSION

Mutations in the sodium channel in pyrethroid-resistant aphids also protect against a fusion protein containing a sodium-channel-specific toxin, in spite of differences in ligand-channel interactions, but do not confer resistance to a fusion protein targeting calcium channels. The use of fusion proteins with differing targets could play a role in managing pesticide resistance.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Yang S
School of Biological and Biomedical Sciences, Durham University, Durham, UK.
Fitches E
Food and Environment Research Agency (Fera), Sand Hutton, York, UK.
Pyati P
School of Biological and Biomedical Sciences, Durham University, Durham, UK.
Gatehouse JA
School of Biological and Biomedical Sciences, Durham University, Durham, UK.

MeSH

AnimalsAphidsCalcium ChannelsInsecticide ResistanceInsecticidesMutationRecombinant Fusion ProteinsSpider VenomsVoltage-Gated Sodium Channels

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25077959

Citation

Yang, Sheng, et al. "Effect of Insecticidal Fusion Proteins Containing Spider Toxins Targeting Sodium and Calcium Ion Channels On Pyrethroid-resistant Strains of Peach-potato Aphid (Myzus Persicae)." Pest Management Science, vol. 71, no. 7, 2015, pp. 951-6.
Yang S, Fitches E, Pyati P, et al. Effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (Myzus persicae). Pest Manag Sci. 2015;71(7):951-6.
Yang, S., Fitches, E., Pyati, P., & Gatehouse, J. A. (2015). Effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (Myzus persicae). Pest Management Science, 71(7), 951-6. https://doi.org/10.1002/ps.3872
Yang S, et al. Effect of Insecticidal Fusion Proteins Containing Spider Toxins Targeting Sodium and Calcium Ion Channels On Pyrethroid-resistant Strains of Peach-potato Aphid (Myzus Persicae). Pest Manag Sci. 2015;71(7):951-6. PubMed PMID: 25077959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (Myzus persicae). AU - Yang,Sheng, AU - Fitches,Elaine, AU - Pyati,Prashant, AU - Gatehouse,John A, Y1 - 2014/08/28/ PY - 2014/03/11/received PY - 2014/06/24/revised PY - 2014/07/25/accepted PY - 2014/8/1/entrez PY - 2014/8/1/pubmed PY - 2016/5/4/medline KW - Homoptera/Hemiptera KW - biopesticide KW - fitness cost KW - insecticide resistance KW - voltage-gated ion channels SP - 951 EP - 6 JF - Pest management science JO - Pest Manag Sci VL - 71 IS - 7 N2 - BACKGROUND: The recombinant fusion proteins Pl1a/GNA and Hv1a/GNA contain the spider venom peptides δ-amaurobitoxin-PI1a or ω-hexatoxin-Hv1a respectively, linked to snowdrop lectin (GNA). Pl1a targets receptor site 4 of insect voltage-gated sodium channels (NaCh), while Hv1a targets voltage-gated calcium channels. Insecticide-resistant strains of peach-potato aphid (Myzus persicae) contain mutations in NaCh. The pyrethroid-resistant kdr (794J) and super-kdr (UKO) strains contain mutations at residues L1014 and M918 in the channel α-subunit respectively, while the kdr + super-kdr strain (4824J), insensitive to pyrethroids, contains mutations at both L1014 and M918. RESULTS: Pl1a/GNA and Hv1a/GNA fusion proteins have estimated LC50 values of 0.35 and 0.19 mg mL(-1) when fed to wild-type M. persicae. For insecticide-resistant aphids, LC50 for the Pl1a/GNA fusion protein increased by 2-6-fold, correlating with pyrethroid resistance (wild type < kdr < super-kdr < kdr + super-kdr strains). In contrast, LC50 for the Hv1a/GNA fusion protein showed limited correlation with pyrethroid resistance. CONCLUSION: Mutations in the sodium channel in pyrethroid-resistant aphids also protect against a fusion protein containing a sodium-channel-specific toxin, in spite of differences in ligand-channel interactions, but do not confer resistance to a fusion protein targeting calcium channels. The use of fusion proteins with differing targets could play a role in managing pesticide resistance. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/25077959/Effect_of_insecticidal_fusion_proteins_containing_spider_toxins_targeting_sodium_and_calcium_ion_channels_on_pyrethroid_resistant_strains_of_peach_potato_aphid__Myzus_persicae__ DB - PRIME DP - Unbound Medicine ER -