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Morphological and phylogenetic diversity of thermophilic cyanobacteria in Algerian hot springs.
Extremophiles. 2014 Nov; 18(6):1035-47.E

Abstract

Geothermal springs in Algeria have been known since the Roman Empire. They mainly locate in Eastern Algeria and are inhabited by thermophilic organisms, which include cyanobacteria forming mats and concretions. In this work, we have investigated the cyanobacterial diversity of these springs. Cyanobacteria were collected from water, concretions and mats in nine hot springs with water temperatures ranging from 39 to 93 °C. Samples were collected for isolation in culture, microscopic morphological examination, and molecular diversity analysis based on 16S rRNA gene sequences. Nineteen different cyanobacterial morphotypes were identified, the most abundant of which were three species of Leptolyngbya, accompanied by members of the genera Gloeocapsa, Gloeocapsopsis, Stigonema, Fischerella, Synechocystis, Microcoleus, Cyanobacterium, Chroococcus and Geitlerinema. Molecular diversity analyses were in good general agreement with classical identification and allowed the detection of additional species in three springs with temperatures higher than 50 °C. They corresponded to a Synechococcus clade and to relatives of the intracellularly calcifying Candidatus Gloeomargarita lithophora. The hottest springs were dominated by members of Leptolyngbya, Synechococcus-like cyanobacteria and Gloeomargarita, whereas Oscillatoriales other than Leptolyngbya, Chroococcales and Stigonematales dominated lower temperature springs. The isolation of some of these strains sets the ground for future studies on the biology of thermophilic cyanobacteria.

Authors+Show Affiliations

Nuclear Research Center of Algiers, Bd. Frantz Fanon, BP 399 Algiers-RP, 16000, Algiers, Algeria, sam.wassi.amarouche@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25078728

Citation

Amarouche-Yala, Samia, et al. "Morphological and Phylogenetic Diversity of Thermophilic Cyanobacteria in Algerian Hot Springs." Extremophiles : Life Under Extreme Conditions, vol. 18, no. 6, 2014, pp. 1035-47.
Amarouche-Yala S, Benouadah A, El Ouahab Bentabet A, et al. Morphological and phylogenetic diversity of thermophilic cyanobacteria in Algerian hot springs. Extremophiles. 2014;18(6):1035-47.
Amarouche-Yala, S., Benouadah, A., El Ouahab Bentabet, A., & López-García, P. (2014). Morphological and phylogenetic diversity of thermophilic cyanobacteria in Algerian hot springs. Extremophiles : Life Under Extreme Conditions, 18(6), 1035-47. https://doi.org/10.1007/s00792-014-0680-7
Amarouche-Yala S, et al. Morphological and Phylogenetic Diversity of Thermophilic Cyanobacteria in Algerian Hot Springs. Extremophiles. 2014;18(6):1035-47. PubMed PMID: 25078728.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological and phylogenetic diversity of thermophilic cyanobacteria in Algerian hot springs. AU - Amarouche-Yala,Samia, AU - Benouadah,Ali, AU - El Ouahab Bentabet,Abd, AU - López-García,Purificación, Y1 - 2014/07/31/ PY - 2014/03/19/received PY - 2014/07/13/accepted PY - 2014/8/1/entrez PY - 2014/8/1/pubmed PY - 2015/7/24/medline SP - 1035 EP - 47 JF - Extremophiles : life under extreme conditions JO - Extremophiles VL - 18 IS - 6 N2 - Geothermal springs in Algeria have been known since the Roman Empire. They mainly locate in Eastern Algeria and are inhabited by thermophilic organisms, which include cyanobacteria forming mats and concretions. In this work, we have investigated the cyanobacterial diversity of these springs. Cyanobacteria were collected from water, concretions and mats in nine hot springs with water temperatures ranging from 39 to 93 °C. Samples were collected for isolation in culture, microscopic morphological examination, and molecular diversity analysis based on 16S rRNA gene sequences. Nineteen different cyanobacterial morphotypes were identified, the most abundant of which were three species of Leptolyngbya, accompanied by members of the genera Gloeocapsa, Gloeocapsopsis, Stigonema, Fischerella, Synechocystis, Microcoleus, Cyanobacterium, Chroococcus and Geitlerinema. Molecular diversity analyses were in good general agreement with classical identification and allowed the detection of additional species in three springs with temperatures higher than 50 °C. They corresponded to a Synechococcus clade and to relatives of the intracellularly calcifying Candidatus Gloeomargarita lithophora. The hottest springs were dominated by members of Leptolyngbya, Synechococcus-like cyanobacteria and Gloeomargarita, whereas Oscillatoriales other than Leptolyngbya, Chroococcales and Stigonematales dominated lower temperature springs. The isolation of some of these strains sets the ground for future studies on the biology of thermophilic cyanobacteria. SN - 1433-4909 UR - https://www.unboundmedicine.com/medline/citation/25078728/Morphological_and_phylogenetic_diversity_of_thermophilic_cyanobacteria_in_Algerian_hot_springs_ L2 - https://dx.doi.org/10.1007/s00792-014-0680-7 DB - PRIME DP - Unbound Medicine ER -