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CRF-R2 and the heterosynaptic regulation of VTA glutamate during reinstatement of cocaine seeking.
J Neurosci. 2014 Jul 30; 34(31):10402-14.JN

Abstract

Stress can reinstate cocaine seeking through an interaction between the stress hormone corticotropin releasing factor (CRF) and glutamate release onto dopamine neurons in the ventral tegmental area (VTA). To better understand the underlying causes, synaptic mechanisms were investigated in brain slices from rats. In control tissue, EPSCs displayed concentration-dependent, bimodal responses to CRF potentiation at low concentrations (3-100 nm) and attenuation at higher concentrations (300 nm). EPSC potentiation and attenuation were mediated by CRF-R1 and CRF-R2 receptor subtypes, respectively, localized to presynaptic terminals. The CRF-R2 attenuation was blocked by the GABA-B receptor antagonist CGP55843. Additional recordings of GABA-A IPSCs showed CRF-R2 activation-facilitated presynaptic release of GABA, suggesting that CRF-R2 may regulate glutamate release via heterosynaptic facilitation of GABA synapses. After chronic cocaine self-administration and extinction training, the sensitivity of glutamate and GABA receptors was unchanged. However, the ability of CRF-R2 agonists to depress EPSCs and potentiate IPSCs was diminished. After yohimbine plus cue reinstatement, the actions of CRF-R2 on GABA and glutamate release were reversed. CRF-R2 activation increased EPSCs as a result of a reduction of tonic GABA-dependent inhibition. After reinstatement, application of the A1 adenosine antagonist 1,3-dipropyl-8-cyclopentylxanthine increased GABA tone to inhibit the CRF-R2 action. Blockade of GABA-B receptors prevented both the CRF-R2 increase in EPSCs and the attenuation produced by 1,3-dipropyl-8-cyclopentylxanthine. These studies demonstrate that presynaptic CRF-R1/R2 tightly regulate glutamate transmission in the VTA via a concerted, heterosynaptic manner that may become altered by stress-related pathologies, such as addiction.

Authors+Show Affiliations

Medical University of South Carolina, Charleston, South Carolina 29425.Medical University of South Carolina, Charleston, South Carolina 29425.Medical University of South Carolina, Charleston, South Carolina 29425 Riegel@musc.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

25080599

Citation

Williams, Courtney L., et al. "CRF-R2 and the Heterosynaptic Regulation of VTA Glutamate During Reinstatement of Cocaine Seeking." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 34, no. 31, 2014, pp. 10402-14.
Williams CL, Buchta WC, Riegel AC. CRF-R2 and the heterosynaptic regulation of VTA glutamate during reinstatement of cocaine seeking. J Neurosci. 2014;34(31):10402-14.
Williams, C. L., Buchta, W. C., & Riegel, A. C. (2014). CRF-R2 and the heterosynaptic regulation of VTA glutamate during reinstatement of cocaine seeking. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 34(31), 10402-14. https://doi.org/10.1523/JNEUROSCI.0911-13.2014
Williams CL, Buchta WC, Riegel AC. CRF-R2 and the Heterosynaptic Regulation of VTA Glutamate During Reinstatement of Cocaine Seeking. J Neurosci. 2014 Jul 30;34(31):10402-14. PubMed PMID: 25080599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CRF-R2 and the heterosynaptic regulation of VTA glutamate during reinstatement of cocaine seeking. AU - Williams,Courtney L, AU - Buchta,William C, AU - Riegel,Arthur C, PY - 2014/8/1/entrez PY - 2014/8/1/pubmed PY - 2014/9/30/medline KW - CRF KW - GABA KW - addiction KW - dopamine KW - glutamate KW - stress SP - 10402 EP - 14 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J Neurosci VL - 34 IS - 31 N2 - Stress can reinstate cocaine seeking through an interaction between the stress hormone corticotropin releasing factor (CRF) and glutamate release onto dopamine neurons in the ventral tegmental area (VTA). To better understand the underlying causes, synaptic mechanisms were investigated in brain slices from rats. In control tissue, EPSCs displayed concentration-dependent, bimodal responses to CRF potentiation at low concentrations (3-100 nm) and attenuation at higher concentrations (300 nm). EPSC potentiation and attenuation were mediated by CRF-R1 and CRF-R2 receptor subtypes, respectively, localized to presynaptic terminals. The CRF-R2 attenuation was blocked by the GABA-B receptor antagonist CGP55843. Additional recordings of GABA-A IPSCs showed CRF-R2 activation-facilitated presynaptic release of GABA, suggesting that CRF-R2 may regulate glutamate release via heterosynaptic facilitation of GABA synapses. After chronic cocaine self-administration and extinction training, the sensitivity of glutamate and GABA receptors was unchanged. However, the ability of CRF-R2 agonists to depress EPSCs and potentiate IPSCs was diminished. After yohimbine plus cue reinstatement, the actions of CRF-R2 on GABA and glutamate release were reversed. CRF-R2 activation increased EPSCs as a result of a reduction of tonic GABA-dependent inhibition. After reinstatement, application of the A1 adenosine antagonist 1,3-dipropyl-8-cyclopentylxanthine increased GABA tone to inhibit the CRF-R2 action. Blockade of GABA-B receptors prevented both the CRF-R2 increase in EPSCs and the attenuation produced by 1,3-dipropyl-8-cyclopentylxanthine. These studies demonstrate that presynaptic CRF-R1/R2 tightly regulate glutamate transmission in the VTA via a concerted, heterosynaptic manner that may become altered by stress-related pathologies, such as addiction. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/25080599/CRF_R2_and_the_heterosynaptic_regulation_of_VTA_glutamate_during_reinstatement_of_cocaine_seeking_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=25080599 DB - PRIME DP - Unbound Medicine ER -