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Methodological issues in the study of intestinal microbiota in irritable bowel syndrome.
World J Gastroenterol. 2014 Jul 21; 20(27):8821-36.WJ

Abstract

Irritable bowel syndrome (IBS) is an intestinal functional disorder with the highest prevalence in the industrialized world. The intestinal microbiota (IM) plays a role in the pathogenesis of IBS and is not merely a consequence of this disorder. Previous research efforts have not revealed unequivocal microbiological signatures of IBS, and the experimental results are contradictory. The experimental methodologies adopted to investigate the complex intestinal ecosystem drastically impact the quality and significance of the results. Therefore, to consider the methodological aspects of the research on IM in IBS, we reviewed 29 relevant original research articles identified through a PubMed search using three combinations of keywords: "irritable bowel syndrome + microflora", "irritable bowel syndrome + microbiota" and "irritable bowel syndrome + microbiome". For each study, we reviewed the quality and significance of the scientific evidence obtained with respect to the experimental method adopted. The data obtained from each study were compared with all considered publications to identify potential inconsistencies and explain contradictory results. The analytical revision of the studies referenced in the present review has contributed to the identification of microbial groups whose relative abundance significantly alters IBS, suggesting that these microbial groups could be IM signatures for this syndrome. The identification of microbial biomarkers in the IM can be advantageous for the development of new diagnostic tools and novel therapeutic strategies for the treatment of different subtypes of IBS.

Authors+Show Affiliations

Valentina Taverniti, Simone Guglielmetti, Division of Food Microbiology and Bioprocessing, Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, 20133 Milan, Italy.Valentina Taverniti, Simone Guglielmetti, Division of Food Microbiology and Bioprocessing, Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, 20133 Milan, Italy.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

25083056

Citation

Taverniti, Valentina, and Simone Guglielmetti. "Methodological Issues in the Study of Intestinal Microbiota in Irritable Bowel Syndrome." World Journal of Gastroenterology, vol. 20, no. 27, 2014, pp. 8821-36.
Taverniti V, Guglielmetti S. Methodological issues in the study of intestinal microbiota in irritable bowel syndrome. World J Gastroenterol. 2014;20(27):8821-36.
Taverniti, V., & Guglielmetti, S. (2014). Methodological issues in the study of intestinal microbiota in irritable bowel syndrome. World Journal of Gastroenterology, 20(27), 8821-36. https://doi.org/10.3748/wjg.v20.i27.8821
Taverniti V, Guglielmetti S. Methodological Issues in the Study of Intestinal Microbiota in Irritable Bowel Syndrome. World J Gastroenterol. 2014 Jul 21;20(27):8821-36. PubMed PMID: 25083056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methodological issues in the study of intestinal microbiota in irritable bowel syndrome. AU - Taverniti,Valentina, AU - Guglielmetti,Simone, PY - 2013/10/31/received PY - 2014/04/10/revised PY - 2014/06/02/accepted PY - 2014/8/2/entrez PY - 2014/8/2/pubmed PY - 2015/4/18/medline KW - Bifidobacteria KW - Intestinal dysfunction KW - Intestinal microbiota KW - Irritable bowel syndrome KW - New generation DNA sequencing SP - 8821 EP - 36 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 20 IS - 27 N2 - Irritable bowel syndrome (IBS) is an intestinal functional disorder with the highest prevalence in the industrialized world. The intestinal microbiota (IM) plays a role in the pathogenesis of IBS and is not merely a consequence of this disorder. Previous research efforts have not revealed unequivocal microbiological signatures of IBS, and the experimental results are contradictory. The experimental methodologies adopted to investigate the complex intestinal ecosystem drastically impact the quality and significance of the results. Therefore, to consider the methodological aspects of the research on IM in IBS, we reviewed 29 relevant original research articles identified through a PubMed search using three combinations of keywords: "irritable bowel syndrome + microflora", "irritable bowel syndrome + microbiota" and "irritable bowel syndrome + microbiome". For each study, we reviewed the quality and significance of the scientific evidence obtained with respect to the experimental method adopted. The data obtained from each study were compared with all considered publications to identify potential inconsistencies and explain contradictory results. The analytical revision of the studies referenced in the present review has contributed to the identification of microbial groups whose relative abundance significantly alters IBS, suggesting that these microbial groups could be IM signatures for this syndrome. The identification of microbial biomarkers in the IM can be advantageous for the development of new diagnostic tools and novel therapeutic strategies for the treatment of different subtypes of IBS. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/25083056/Methodological_issues_in_the_study_of_intestinal_microbiota_in_irritable_bowel_syndrome_ L2 - https://www.wjgnet.com/1007-9327/full/v20/i27/8821.htm DB - PRIME DP - Unbound Medicine ER -