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Generation of triplet carbonyl compounds during peroxidase catalysed reactions.
J Biolumin Chemilumin 1989; 4(1):193-9JB

Abstract

Peroxidases, acting as oxidase upon appropriate substrates, generate carbonyl compounds in the electronically excited triplet state. These excited species can transfer energy as demonstrated by the appearance of the acceptor fluorescence or induced photochemistry concomitant with the disappearance of phosphorescence. Chlorophyll, an efficient emissive acceptor, either naturally present or artificially incorporated into organelles and cells, allows the in situ detection of biologically generated excited species. With neutrophils, the myeloperoxidase promoted acetone phosphorescence can readily be detected. In other cases, e.g. triplet benzaldehyde, it is possible to observe emission from lipid peroxidation initiated by the triplet carbonyl compound.

Authors+Show Affiliations

Department of Biochemistry, Universidade de São Paulo, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

2508435

Citation

Cilento, G. "Generation of Triplet Carbonyl Compounds During Peroxidase Catalysed Reactions." Journal of Bioluminescence and Chemiluminescence, vol. 4, no. 1, 1989, pp. 193-9.
Cilento G. Generation of triplet carbonyl compounds during peroxidase catalysed reactions. J Biolumin Chemilumin. 1989;4(1):193-9.
Cilento, G. (1989). Generation of triplet carbonyl compounds during peroxidase catalysed reactions. Journal of Bioluminescence and Chemiluminescence, 4(1), pp. 193-9.
Cilento G. Generation of Triplet Carbonyl Compounds During Peroxidase Catalysed Reactions. J Biolumin Chemilumin. 1989;4(1):193-9. PubMed PMID: 2508435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of triplet carbonyl compounds during peroxidase catalysed reactions. A1 - Cilento,G, PY - 1989/7/1/pubmed PY - 1989/7/1/medline PY - 1989/7/1/entrez SP - 193 EP - 9 JF - Journal of bioluminescence and chemiluminescence JO - J. Biolumin. Chemilumin. VL - 4 IS - 1 N2 - Peroxidases, acting as oxidase upon appropriate substrates, generate carbonyl compounds in the electronically excited triplet state. These excited species can transfer energy as demonstrated by the appearance of the acceptor fluorescence or induced photochemistry concomitant with the disappearance of phosphorescence. Chlorophyll, an efficient emissive acceptor, either naturally present or artificially incorporated into organelles and cells, allows the in situ detection of biologically generated excited species. With neutrophils, the myeloperoxidase promoted acetone phosphorescence can readily be detected. In other cases, e.g. triplet benzaldehyde, it is possible to observe emission from lipid peroxidation initiated by the triplet carbonyl compound. SN - 0884-3996 UR - https://www.unboundmedicine.com/medline/citation/2508435/Generation_of_triplet_carbonyl_compounds_during_peroxidase_catalysed_reactions_ L2 - https://doi.org/10.1002/bio.1170040128 DB - PRIME DP - Unbound Medicine ER -