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Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells.

Abstract

BACKGROUND

In Europe, extracts of Equisetum arvense (common horsetail) have a long tradition in the treatment of inflammatory disorders. To understand the molecular basis for its use, we investigated the immunomodulatory capacity of a standardized commercially available common horsetail extract on human primary lymphocyte function in vitro.

METHODS

The standardized extract of Equisetum arvense was phytochemically characterized. Effects on proliferation, viability and activity of mitogen-activated human lymphocytes were assessed in comparison to cyclosporine A using annexin V/propidium iodide staining assays and flow cytometry-based surface receptor characterization, respectively. Intracellular levels of effector molecules (IL-2, IFN-γ and TNF-α) were analyzed with cytokine assays.

RESULTS

T cell proliferation was inhibited dose dependently by the Equisetum extract without induction of apoptosis or necrosis. This effect was mediated through inhibition of lymphocyte activation, specifically by diminishing CD69 and IL-2 surface receptor expression and intracellular IL-2 production. Furthermore, treatment with Equisetum arvense inhibited effector functions, as indicated by reduced production of IFN-γ and TNF-α.

CONCLUSIONS

The data indicate that the used extract of Equisetum arvense interferes with the polyfunctionality of immunocompetent cells thereby providing an anti-inflammatory mode-of-action.

Authors+Show Affiliations

Center for Complementary Medicine, Department of Environmental Health Sciences, Medical Center, University of Freiburg, Breisacherstr, 115B, 79106 Freiburg, Germany. carsten.gruendemann@uniklinik-freiburg.de.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25088216

Citation

Gründemann, Carsten, et al. "Equisetum Arvense (common Horsetail) Modulates the Function of Inflammatory Immunocompetent Cells." BMC Complementary and Alternative Medicine, vol. 14, 2014, p. 283.
Gründemann C, Lengen K, Sauer B, et al. Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells. BMC Complement Altern Med. 2014;14:283.
Gründemann, C., Lengen, K., Sauer, B., Garcia-Käufer, M., Zehl, M., & Huber, R. (2014). Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells. BMC Complementary and Alternative Medicine, 14, p. 283. doi:10.1186/1472-6882-14-283.
Gründemann C, et al. Equisetum Arvense (common Horsetail) Modulates the Function of Inflammatory Immunocompetent Cells. BMC Complement Altern Med. 2014 Aug 4;14:283. PubMed PMID: 25088216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells. AU - Gründemann,Carsten, AU - Lengen,Karin, AU - Sauer,Barbara, AU - Garcia-Käufer,Manuel, AU - Zehl,Martin, AU - Huber,Roman, Y1 - 2014/08/04/ PY - 2014/03/12/received PY - 2014/07/22/accepted PY - 2014/8/5/entrez PY - 2014/8/5/pubmed PY - 2014/11/15/medline SP - 283 EP - 283 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 14 N2 - BACKGROUND: In Europe, extracts of Equisetum arvense (common horsetail) have a long tradition in the treatment of inflammatory disorders. To understand the molecular basis for its use, we investigated the immunomodulatory capacity of a standardized commercially available common horsetail extract on human primary lymphocyte function in vitro. METHODS: The standardized extract of Equisetum arvense was phytochemically characterized. Effects on proliferation, viability and activity of mitogen-activated human lymphocytes were assessed in comparison to cyclosporine A using annexin V/propidium iodide staining assays and flow cytometry-based surface receptor characterization, respectively. Intracellular levels of effector molecules (IL-2, IFN-γ and TNF-α) were analyzed with cytokine assays. RESULTS: T cell proliferation was inhibited dose dependently by the Equisetum extract without induction of apoptosis or necrosis. This effect was mediated through inhibition of lymphocyte activation, specifically by diminishing CD69 and IL-2 surface receptor expression and intracellular IL-2 production. Furthermore, treatment with Equisetum arvense inhibited effector functions, as indicated by reduced production of IFN-γ and TNF-α. CONCLUSIONS: The data indicate that the used extract of Equisetum arvense interferes with the polyfunctionality of immunocompetent cells thereby providing an anti-inflammatory mode-of-action. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/25088216/Equisetum_arvense__common_horsetail__modulates_the_function_of_inflammatory_immunocompetent_cells_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-283 DB - PRIME DP - Unbound Medicine ER -