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Effects of salinity and prolactin on gene transcript levels of ion transporters, ion pumps and prolactin receptors in Mozambique tilapia intestine.
Gen Comp Endocrinol 2014; 206:146-54GC

Abstract

Euryhaline teleosts are faced with significant challenges during changes in salinity. Osmoregulatory responses to salinity changes are mediated through the neuroendocrine system which directs osmoregulatory tissues to modulate ion transport. Prolactin (PRL) plays a major role in freshwater (FW) osmoregulation by promoting ion uptake in osmoregulatory tissues, including intestine. We measured mRNA expression of ion pumps, Na(+)/K(+)-ATPase α3-subunit (NKAα3) and vacuolar type H(+)-ATPase A-subunit (V-ATPase A-subunit); ion transporters/channels, Na(+)/K(+)/2Cl(-) co-transporter (NKCC2) and cystic fibrosis transmembrane conductance regulator (CFTR); and the two PRL receptors, PRLR1 and PRLR2 in eleven intestinal segments of Mozambique tilapia (Oreochromis mossambicus) acclimated to FW or seawater (SW). Gene expression levels of NKAα3, V-ATPase A-subunit, and NKCC2 were generally lower in middle segments of the intestine, whereas CFTR mRNA was most highly expressed in anterior intestine of FW-fish. In both FW- and SW-acclimated fish, PRLR1 was most highly expressed in the terminal segment of the intestine, whereas PRLR2 was generally most highly expressed in anterior intestinal segments. While NKCC2, NKAα3 and PRLR2 mRNA expression was higher in the intestinal segments of SW-acclimated fish, CFTR mRNA expression was higher in FW-fish; PRLR1 and V-ATPase A-subunit mRNA expression was similar between FW- and SW-acclimated fish. Next, we characterized the effects of hypophysectomy (Hx) and PRL replacement on the expression of intestinal transcripts. Hypophysectomy reduced both NKCC2 and CFTR expression in particular intestinal segments; however, only NKCC2 expression was restored by PRL replacement. Together, these findings describe how both acclimation salinity and PRL impact transcript levels of effectors of ion transport in tilapia intestine.

Authors+Show Affiliations

Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA. Electronic address: seale@hawaii.edu.Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA; Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA.Department of Biology & Center for Neuroendocrine Studies, University of Massachusetts, Amherst, MA 01003, USA.Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.Department of Poultry and Aquaculture, The Volcani Center, Agricultural Research Organization, Bet Dagan 50250, Israel.Department of Poultry and Aquaculture, The Volcani Center, Agricultural Research Organization, Bet Dagan 50250, Israel.Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA; University of Hawaii Sea Grant College Program, University of Hawaii at Manoa, Honolulu, HI 96822, USA.Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25088575

Citation

Seale, Andre P., et al. "Effects of Salinity and Prolactin On Gene Transcript Levels of Ion Transporters, Ion Pumps and Prolactin Receptors in Mozambique Tilapia Intestine." General and Comparative Endocrinology, vol. 206, 2014, pp. 146-54.
Seale AP, Stagg JJ, Yamaguchi Y, et al. Effects of salinity and prolactin on gene transcript levels of ion transporters, ion pumps and prolactin receptors in Mozambique tilapia intestine. Gen Comp Endocrinol. 2014;206:146-54.
Seale, A. P., Stagg, J. J., Yamaguchi, Y., Breves, J. P., Soma, S., Watanabe, S., ... Grau, E. G. (2014). Effects of salinity and prolactin on gene transcript levels of ion transporters, ion pumps and prolactin receptors in Mozambique tilapia intestine. General and Comparative Endocrinology, 206, pp. 146-54. doi:10.1016/j.ygcen.2014.07.020.
Seale AP, et al. Effects of Salinity and Prolactin On Gene Transcript Levels of Ion Transporters, Ion Pumps and Prolactin Receptors in Mozambique Tilapia Intestine. Gen Comp Endocrinol. 2014 Sep 15;206:146-54. PubMed PMID: 25088575.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of salinity and prolactin on gene transcript levels of ion transporters, ion pumps and prolactin receptors in Mozambique tilapia intestine. AU - Seale,Andre P, AU - Stagg,Jacob J, AU - Yamaguchi,Yoko, AU - Breves,Jason P, AU - Soma,Satoshi, AU - Watanabe,Soichi, AU - Kaneko,Toyoji, AU - Cnaani,Avner, AU - Harpaz,Sheenan, AU - Lerner,Darren T, AU - Grau,E Gordon, Y1 - 2014/08/01/ PY - 2013/08/28/received PY - 2014/05/23/revised PY - 2014/07/07/accepted PY - 2014/8/5/entrez PY - 2014/8/5/pubmed PY - 2014/12/17/medline KW - Intestine KW - Ion transporters KW - Osmoregulation KW - Prolactin KW - Salinity KW - Tilapia SP - 146 EP - 54 JF - General and comparative endocrinology JO - Gen. Comp. Endocrinol. VL - 206 N2 - Euryhaline teleosts are faced with significant challenges during changes in salinity. Osmoregulatory responses to salinity changes are mediated through the neuroendocrine system which directs osmoregulatory tissues to modulate ion transport. Prolactin (PRL) plays a major role in freshwater (FW) osmoregulation by promoting ion uptake in osmoregulatory tissues, including intestine. We measured mRNA expression of ion pumps, Na(+)/K(+)-ATPase α3-subunit (NKAα3) and vacuolar type H(+)-ATPase A-subunit (V-ATPase A-subunit); ion transporters/channels, Na(+)/K(+)/2Cl(-) co-transporter (NKCC2) and cystic fibrosis transmembrane conductance regulator (CFTR); and the two PRL receptors, PRLR1 and PRLR2 in eleven intestinal segments of Mozambique tilapia (Oreochromis mossambicus) acclimated to FW or seawater (SW). Gene expression levels of NKAα3, V-ATPase A-subunit, and NKCC2 were generally lower in middle segments of the intestine, whereas CFTR mRNA was most highly expressed in anterior intestine of FW-fish. In both FW- and SW-acclimated fish, PRLR1 was most highly expressed in the terminal segment of the intestine, whereas PRLR2 was generally most highly expressed in anterior intestinal segments. While NKCC2, NKAα3 and PRLR2 mRNA expression was higher in the intestinal segments of SW-acclimated fish, CFTR mRNA expression was higher in FW-fish; PRLR1 and V-ATPase A-subunit mRNA expression was similar between FW- and SW-acclimated fish. Next, we characterized the effects of hypophysectomy (Hx) and PRL replacement on the expression of intestinal transcripts. Hypophysectomy reduced both NKCC2 and CFTR expression in particular intestinal segments; however, only NKCC2 expression was restored by PRL replacement. Together, these findings describe how both acclimation salinity and PRL impact transcript levels of effectors of ion transport in tilapia intestine. SN - 1095-6840 UR - https://www.unboundmedicine.com/medline/citation/25088575/Effects_of_salinity_and_prolactin_on_gene_transcript_levels_of_ion_transporters_ion_pumps_and_prolactin_receptors_in_Mozambique_tilapia_intestine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-6480(14)00293-7 DB - PRIME DP - Unbound Medicine ER -