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MicroRNA-150 predicts a favorable prognosis in patients with epithelial ovarian cancer, and inhibits cell invasion and metastasis by suppressing transcriptional repressor ZEB1.
PLoS One. 2014; 9(8):e103965.Plos

Abstract

MicroRNA (miR)-150 has been reported to be dramatically downregulated in human epithelial ovarian cancer (EOC) tissues and patients' serum compared to normal controls. This study aimed to investigate clinical significance and molecular mechanisms of miR-150 in EOC. In the current study, quantitative real-time PCR analysis showed that miR-150 was significantly downregulated in human EOC tissues compared to normal tissue samples. Then, we demonstrated the significant associations of miR-150 downregulation with aggressive clinicopathological features of EOC patients, including high clinical stage and pathological grade, and shorter overall and progression-free survivals. More importantly, the multivariate analysis identified miR-150 expression as an independent prognostic biomarker in EOC. After that, luciferase reporter assays demonstrated that Zinc Finger E-Box Binding Homeobox 1 (ZEB1), a crucial regulator of epithelial-to-mesenchymal transition (EMT), was a direct target of miR-150 in EOC cells. Moreover, we found that the ectopic expression of miR-150 could efficiently inhibit cell proliferation, invasion and metastasis by suppressing the expression of ZEB1. Furthermore, we also observed a significantly negative correlation between miR-150 and ZEB1 mRNA expression in EOC tissues (rs = -0.45, P<0.001). In conclusion, these findings offer the convincing evidence that aberrant expression of miR-150 may play a role in tumor progression and prognosis in patients with EOC. Moreover, our data reveal that miR-150 may function as a tumor suppressor and modulate EOC cell proliferation, and invasion by directly and negatively regulating ZEB1, implying the re-expression of miR-150 might be a potential therapeutic strategy for EOC.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.Department of Obstetrics and Gynecology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.Department of Obstetrics and Gynecology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.Department of Obstetrics and Gynecology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.Department of Obstetrics and Gynecology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25090005

Citation

Jin, Minfei, et al. "MicroRNA-150 Predicts a Favorable Prognosis in Patients With Epithelial Ovarian Cancer, and Inhibits Cell Invasion and Metastasis By Suppressing Transcriptional Repressor ZEB1." PloS One, vol. 9, no. 8, 2014, pp. e103965.
Jin M, Yang Z, Ye W, et al. MicroRNA-150 predicts a favorable prognosis in patients with epithelial ovarian cancer, and inhibits cell invasion and metastasis by suppressing transcriptional repressor ZEB1. PLoS ONE. 2014;9(8):e103965.
Jin, M., Yang, Z., Ye, W., Xu, H., & Hua, X. (2014). MicroRNA-150 predicts a favorable prognosis in patients with epithelial ovarian cancer, and inhibits cell invasion and metastasis by suppressing transcriptional repressor ZEB1. PloS One, 9(8), e103965. https://doi.org/10.1371/journal.pone.0103965
Jin M, et al. MicroRNA-150 Predicts a Favorable Prognosis in Patients With Epithelial Ovarian Cancer, and Inhibits Cell Invasion and Metastasis By Suppressing Transcriptional Repressor ZEB1. PLoS ONE. 2014;9(8):e103965. PubMed PMID: 25090005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MicroRNA-150 predicts a favorable prognosis in patients with epithelial ovarian cancer, and inhibits cell invasion and metastasis by suppressing transcriptional repressor ZEB1. AU - Jin,Minfei, AU - Yang,Zujing, AU - Ye,Weiping, AU - Xu,Hongling, AU - Hua,Xiaolin, Y1 - 2014/08/04/ PY - 2014/05/25/received PY - 2014/07/09/accepted PY - 2014/8/5/entrez PY - 2014/8/5/pubmed PY - 2015/4/15/medline SP - e103965 EP - e103965 JF - PloS one JO - PLoS ONE VL - 9 IS - 8 N2 - MicroRNA (miR)-150 has been reported to be dramatically downregulated in human epithelial ovarian cancer (EOC) tissues and patients' serum compared to normal controls. This study aimed to investigate clinical significance and molecular mechanisms of miR-150 in EOC. In the current study, quantitative real-time PCR analysis showed that miR-150 was significantly downregulated in human EOC tissues compared to normal tissue samples. Then, we demonstrated the significant associations of miR-150 downregulation with aggressive clinicopathological features of EOC patients, including high clinical stage and pathological grade, and shorter overall and progression-free survivals. More importantly, the multivariate analysis identified miR-150 expression as an independent prognostic biomarker in EOC. After that, luciferase reporter assays demonstrated that Zinc Finger E-Box Binding Homeobox 1 (ZEB1), a crucial regulator of epithelial-to-mesenchymal transition (EMT), was a direct target of miR-150 in EOC cells. Moreover, we found that the ectopic expression of miR-150 could efficiently inhibit cell proliferation, invasion and metastasis by suppressing the expression of ZEB1. Furthermore, we also observed a significantly negative correlation between miR-150 and ZEB1 mRNA expression in EOC tissues (rs = -0.45, P<0.001). In conclusion, these findings offer the convincing evidence that aberrant expression of miR-150 may play a role in tumor progression and prognosis in patients with EOC. Moreover, our data reveal that miR-150 may function as a tumor suppressor and modulate EOC cell proliferation, and invasion by directly and negatively regulating ZEB1, implying the re-expression of miR-150 might be a potential therapeutic strategy for EOC. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25090005/MicroRNA_150_predicts_a_favorable_prognosis_in_patients_with_epithelial_ovarian_cancer_and_inhibits_cell_invasion_and_metastasis_by_suppressing_transcriptional_repressor_ZEB1_ L2 - http://dx.plos.org/10.1371/journal.pone.0103965 DB - PRIME DP - Unbound Medicine ER -