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PU.1 promotes miR-191 to inhibit adipogenesis in 3T3-L1 preadipocytes.
Biochem Biophys Res Commun. 2014 Aug 22; 451(2):329-33.BB

Abstract

PU.1, an Ets family transcription factor, was previously demonstrated expressed in 3T3-L1 preadipocytes and had an negative effect on adiopogenesis. However, the underlying mechanism remains elusive. Here, miR-191 was identified as an inhibitor of adipocyte differentiation through targeting the 3' untranslated regions of C/EBPβ, the initial factor in the C/EBPα/β-PPARγ terminal pathway of adipogenic differentiation. MiR-191 suppressed the lipid accumulation by Oil Red O staining and downregulated the levels of adipogenic marker genes PPARγ (P<0.01), aP2 (P<0.01) and FAS (P<0.05). Then, we found that PU.1 overexpression resulted in upregulation of miR-191 and adipogenic inhibition. Likewise, PU.1 overexpression rescued the miR-191 decrease and resisted the adipogenic promotion caused by miR-191 oligonucleotide inhibitor. Collectively, these results revealed that PU.1 promoted miR-191 to suppress adipogenesis 3T3-L1 preadipocyte and indicated a new mechanism of PU.1 inhibiting adipogenesis.

Authors+Show Affiliations

College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.Beijing Shuita Aged-vinegar Research Institute for Biosciences, Beijing 102200, PR China.College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China. Electronic address: jiziqing2010@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25094047

Citation

Ji, Shengyue, et al. "PU.1 Promotes miR-191 to Inhibit Adipogenesis in 3T3-L1 Preadipocytes." Biochemical and Biophysical Research Communications, vol. 451, no. 2, 2014, pp. 329-33.
Ji S, Li W, Bao L, et al. PU.1 promotes miR-191 to inhibit adipogenesis in 3T3-L1 preadipocytes. Biochem Biophys Res Commun. 2014;451(2):329-33.
Ji, S., Li, W., Bao, L., Han, P., Yang, W., Ma, L., Meng, F., & Cao, B. (2014). PU.1 promotes miR-191 to inhibit adipogenesis in 3T3-L1 preadipocytes. Biochemical and Biophysical Research Communications, 451(2), 329-33. https://doi.org/10.1016/j.bbrc.2014.07.130
Ji S, et al. PU.1 Promotes miR-191 to Inhibit Adipogenesis in 3T3-L1 Preadipocytes. Biochem Biophys Res Commun. 2014 Aug 22;451(2):329-33. PubMed PMID: 25094047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PU.1 promotes miR-191 to inhibit adipogenesis in 3T3-L1 preadipocytes. AU - Ji,Shengyue, AU - Li,Weili, AU - Bao,Lijuan, AU - Han,Peng, AU - Yang,Weiping, AU - Ma,Li, AU - Meng,Fanxu, AU - Cao,Binyun, Y1 - 2014/08/02/ PY - 2014/07/19/received PY - 2014/07/28/accepted PY - 2014/8/6/entrez PY - 2014/8/6/pubmed PY - 2014/10/22/medline KW - 3T3-L1 preadipocytes KW - Adipogenesis KW - C/EBPβ KW - PU.1 KW - miR-191 SP - 329 EP - 33 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 451 IS - 2 N2 - PU.1, an Ets family transcription factor, was previously demonstrated expressed in 3T3-L1 preadipocytes and had an negative effect on adiopogenesis. However, the underlying mechanism remains elusive. Here, miR-191 was identified as an inhibitor of adipocyte differentiation through targeting the 3' untranslated regions of C/EBPβ, the initial factor in the C/EBPα/β-PPARγ terminal pathway of adipogenic differentiation. MiR-191 suppressed the lipid accumulation by Oil Red O staining and downregulated the levels of adipogenic marker genes PPARγ (P<0.01), aP2 (P<0.01) and FAS (P<0.05). Then, we found that PU.1 overexpression resulted in upregulation of miR-191 and adipogenic inhibition. Likewise, PU.1 overexpression rescued the miR-191 decrease and resisted the adipogenic promotion caused by miR-191 oligonucleotide inhibitor. Collectively, these results revealed that PU.1 promoted miR-191 to suppress adipogenesis 3T3-L1 preadipocyte and indicated a new mechanism of PU.1 inhibiting adipogenesis. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/25094047/PU_1_promotes_miR_191_to_inhibit_adipogenesis_in_3T3_L1_preadipocytes_ DB - PRIME DP - Unbound Medicine ER -