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[Pharmacokinetic study of six aconitine alkaloids in aconiti lateralis radix praeparata in beagle dogs].
Zhong Yao Cai. 2014 Feb; 37(2):284-7.ZY

Abstract

OBJECTIVE

To study the pharmacokinetics characteristics of six Aconitum alkaloids aconitine (AC), mesaconitine (MA), hypaconitine (HA), benzoylaconine (BAC), benzoylmesaconine (BMA) and benzoylhypaconine (BHA) in beagle dogs.

METHODS

An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for simultaneous quantitation of six Aconitum alkaloids in beagle dog plasma after oral administration of Aconiti Lateralis Radix Praeparata decoction. UPLC/MS/MS system coupled with an electrospray ionization (ESI) source was performed in multiple-reaction monitoring (MRM) mode. Sample preparation was performed with solid-phase extraction(SPE) on a 3 mL HLB cartridge before the analysis. The separation was applied on a Waters C8 column (100 mm x 2.1 mm, 1.7 microm) and a gradient elution of methanol and 0.2% formic acid-water was used as mobile phase. The pharmacokinetic parameters were calculated by the results of the analysis through the DAS 2. 1 software (Drug and Statistics for Windows).

RESULTS

The results showed that the fitting model for the six Aconitum alkaloids was the one-compartment model pharmacokinetics.

CONCLUSION

The method is successfully used for the pharmacokinetic evaluation of the six Aconitum alkaloids in beagle dog plasma, it can help monitor the ADME/Tox process when taking Aconiti Lateralis Radix Praeparata by observing the pharmacokinetic process. The results provide a good reference for clinical treatment and safe application of Aconiti Lateralis Radix Praeparata.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

25095352

Citation

Xiao, Ri-Ping, et al. "[Pharmacokinetic Study of Six Aconitine Alkaloids in Aconiti Lateralis Radix Praeparata in Beagle Dogs]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 37, no. 2, 2014, pp. 284-7.
Xiao RP, Lai XP, Zhao Y, et al. [Pharmacokinetic study of six aconitine alkaloids in aconiti lateralis radix praeparata in beagle dogs]. Zhong Yao Cai. 2014;37(2):284-7.
Xiao, R. P., Lai, X. P., Zhao, Y., Yu, L. W., Zhu, Y. L., & Li, G. (2014). [Pharmacokinetic study of six aconitine alkaloids in aconiti lateralis radix praeparata in beagle dogs]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 37(2), 284-7.
Xiao RP, et al. [Pharmacokinetic Study of Six Aconitine Alkaloids in Aconiti Lateralis Radix Praeparata in Beagle Dogs]. Zhong Yao Cai. 2014;37(2):284-7. PubMed PMID: 25095352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Pharmacokinetic study of six aconitine alkaloids in aconiti lateralis radix praeparata in beagle dogs]. AU - Xiao,Ri-Ping, AU - Lai,Xiao-Ping, AU - Zhao,Yai, AU - Yu,Liang-Wen, AU - Zhu,Yue-Lan, AU - Li,Geng, PY - 2014/8/7/entrez PY - 2014/8/7/pubmed PY - 2015/1/9/medline SP - 284 EP - 7 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 37 IS - 2 N2 - OBJECTIVE: To study the pharmacokinetics characteristics of six Aconitum alkaloids aconitine (AC), mesaconitine (MA), hypaconitine (HA), benzoylaconine (BAC), benzoylmesaconine (BMA) and benzoylhypaconine (BHA) in beagle dogs. METHODS: An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for simultaneous quantitation of six Aconitum alkaloids in beagle dog plasma after oral administration of Aconiti Lateralis Radix Praeparata decoction. UPLC/MS/MS system coupled with an electrospray ionization (ESI) source was performed in multiple-reaction monitoring (MRM) mode. Sample preparation was performed with solid-phase extraction(SPE) on a 3 mL HLB cartridge before the analysis. The separation was applied on a Waters C8 column (100 mm x 2.1 mm, 1.7 microm) and a gradient elution of methanol and 0.2% formic acid-water was used as mobile phase. The pharmacokinetic parameters were calculated by the results of the analysis through the DAS 2. 1 software (Drug and Statistics for Windows). RESULTS: The results showed that the fitting model for the six Aconitum alkaloids was the one-compartment model pharmacokinetics. CONCLUSION: The method is successfully used for the pharmacokinetic evaluation of the six Aconitum alkaloids in beagle dog plasma, it can help monitor the ADME/Tox process when taking Aconiti Lateralis Radix Praeparata by observing the pharmacokinetic process. The results provide a good reference for clinical treatment and safe application of Aconiti Lateralis Radix Praeparata. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/25095352/[Pharmacokinetic_study_of_six_aconitine_alkaloids_in_aconiti_lateralis_radix_praeparata_in_beagle_dogs]_ DB - PRIME DP - Unbound Medicine ER -