Tags

Type your tag names separated by a space and hit enter

Human telomerase reverse transcriptase messenger RNA (TERT mRNA) as a tumour marker for early detection of hepatocellular carcinoma.
Arab J Gastroenterol. 2014 Jun; 15(2):68-71.AJ

Abstract

BACKGROUND AND STUDY AIMS

Hepatocellular carcinoma (HCC) is the fifth most common malignancy in the world. Although tumour markers such as α-foetoprotein (AFP) are widely used and important for HCC detection in clinical scenes, they still do not provide a satisfactory solution to detect HCC at the early stage. The aim of our study was to illustrate the significance of serum human telomerase reverse transcriptase messenger RNA (hTERT mRNA) as a novel biomarker for early detection of HCC.

PATIENTS AND METHODS

Thirty-five patients with HCC, 15 patients with liver cirrhosis, and 10 healthy subjects were sex and age matched. History taking, full physical examination, and laboratory investigations including liver function tests, hepatitis markers, AFP, and quantification of serum human telomerase reverse transcriptase mRNA (hTERT mRNA) using real-time reverse transcription polymerase chain reaction (RT-PCR) were conducted. Ultrasonography (US), triphasic computed tomography (CT), and liver biopsy were carried out.

RESULTS

The hTERT was above the cutoff point (>144 copies/ml) in 27 HCC patients with a sensitivity of 77.14% and a specificity of 100% and with a positive predictive value (PPV) of 100% and a negative predictive value (NPV) of 65.2%. AFP was above the cutoff point (>50 ng/ml) in 23 HCC patients with a sensitivity of 65.71% and a specificity of 96% and with a PPV of 96.3% and an NPV of 53.8%. Our study also showed a statistically significant relationship between the size of the tumour in HCC patients and both AFP and hTERT, and hTERT appears to be more correlated with the size of the tumour than AFP. There is no direct correlation between hTERT or AFP and the number of focal lesions with p value>0.05.

CONCLUSION

Serum hTERT mRNA is more sensitive and specific than AFP in the early detection of HCC and its level correlates with the size of the tumour.

Authors+Show Affiliations

Department of Internal Medicine, Faculty of Medicine, Cairo University, Giza, Egypt. Electronic address: Ahmed_el_mazny@yahoo.com.Department of Internal Medicine, Faculty of Medicine, Cairo University, Giza, Egypt.Department of Clinical Pathology, Faculty of Medicine, Cairo University, Giza, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25097049

Citation

El-Mazny, Ahmed, et al. "Human Telomerase Reverse Transcriptase Messenger RNA (TERT mRNA) as a Tumour Marker for Early Detection of Hepatocellular Carcinoma." Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology, vol. 15, no. 2, 2014, pp. 68-71.
El-Mazny A, Sayed M, Sharaf S. Human telomerase reverse transcriptase messenger RNA (TERT mRNA) as a tumour marker for early detection of hepatocellular carcinoma. Arab J Gastroenterol. 2014;15(2):68-71.
El-Mazny, A., Sayed, M., & Sharaf, S. (2014). Human telomerase reverse transcriptase messenger RNA (TERT mRNA) as a tumour marker for early detection of hepatocellular carcinoma. Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology, 15(2), 68-71. https://doi.org/10.1016/j.ajg.2014.04.001
El-Mazny A, Sayed M, Sharaf S. Human Telomerase Reverse Transcriptase Messenger RNA (TERT mRNA) as a Tumour Marker for Early Detection of Hepatocellular Carcinoma. Arab J Gastroenterol. 2014;15(2):68-71. PubMed PMID: 25097049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human telomerase reverse transcriptase messenger RNA (TERT mRNA) as a tumour marker for early detection of hepatocellular carcinoma. AU - El-Mazny,Ahmed, AU - Sayed,Mohamed, AU - Sharaf,Sahar, Y1 - 2014/06/10/ PY - 2013/10/03/received PY - 2014/01/04/revised PY - 2014/04/02/accepted PY - 2014/8/7/entrez PY - 2014/8/7/pubmed PY - 2015/4/24/medline KW - Hepatocellular carcinoma KW - Serum human telomerase reverse transcriptase messenger RNA (hTERT mRNA) KW - α-Foetoprotein SP - 68 EP - 71 JF - Arab journal of gastroenterology : the official publication of the Pan-Arab Association of Gastroenterology JO - Arab J Gastroenterol VL - 15 IS - 2 N2 - BACKGROUND AND STUDY AIMS: Hepatocellular carcinoma (HCC) is the fifth most common malignancy in the world. Although tumour markers such as α-foetoprotein (AFP) are widely used and important for HCC detection in clinical scenes, they still do not provide a satisfactory solution to detect HCC at the early stage. The aim of our study was to illustrate the significance of serum human telomerase reverse transcriptase messenger RNA (hTERT mRNA) as a novel biomarker for early detection of HCC. PATIENTS AND METHODS: Thirty-five patients with HCC, 15 patients with liver cirrhosis, and 10 healthy subjects were sex and age matched. History taking, full physical examination, and laboratory investigations including liver function tests, hepatitis markers, AFP, and quantification of serum human telomerase reverse transcriptase mRNA (hTERT mRNA) using real-time reverse transcription polymerase chain reaction (RT-PCR) were conducted. Ultrasonography (US), triphasic computed tomography (CT), and liver biopsy were carried out. RESULTS: The hTERT was above the cutoff point (>144 copies/ml) in 27 HCC patients with a sensitivity of 77.14% and a specificity of 100% and with a positive predictive value (PPV) of 100% and a negative predictive value (NPV) of 65.2%. AFP was above the cutoff point (>50 ng/ml) in 23 HCC patients with a sensitivity of 65.71% and a specificity of 96% and with a PPV of 96.3% and an NPV of 53.8%. Our study also showed a statistically significant relationship between the size of the tumour in HCC patients and both AFP and hTERT, and hTERT appears to be more correlated with the size of the tumour than AFP. There is no direct correlation between hTERT or AFP and the number of focal lesions with p value>0.05. CONCLUSION: Serum hTERT mRNA is more sensitive and specific than AFP in the early detection of HCC and its level correlates with the size of the tumour. SN - 2090-2387 UR - https://www.unboundmedicine.com/medline/citation/25097049/Human_telomerase_reverse_transcriptase_messenger_RNA__TERT_mRNA__as_a_tumour_marker_for_early_detection_of_hepatocellular_carcinoma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1687-1979(14)00037-9 DB - PRIME DP - Unbound Medicine ER -