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Involvement of anandamide transporter in calcitonin gene-related peptide expression stimulated by nitroglycerin and influence of ALDH2 Glu504Lys polymorphism.
J Cardiovasc Pharmacol. 2014 Nov; 64(5):460-4.JC

Abstract

The aim of this study was to investigate whether N-arachidonic acid ethanolamine (anandamide, AEA) transporter contributed to calcitonin gene-related peptide (CGRP) expression mediated by nitroglycerin (GTN) in peripheral blood mononuclear cells (PBMCs) of healthy volunteers and its association with the mitochondrial aldehyde dehydrogenase-2 (ALDH2) Glu504Lys (ALDH2*2) polymorphism. In 10 ALDH2*2-genotyped Chinese volunteers, we assessed the activity of AEA transporter and expression of CGRP messenger ribonucleic acid (mRNA) in cultured PBMCs treated with different concentration of GTN with or without pretreatment with AM404 (the AEA transporter blocker). In this study, the activity of AEA transporter and expression of CGRP mRNA elevated with the increase in the concentration of GTN. Pretreatment of the cells with AM404 (1 μM) 2 hours before GTN reduced the GTN-induced increase in both AEA transporter activity and CGRP mRNA expression significantly, and cells with the ALDH2*1/*1 homozygote genotype showed significantly higher activity of AEA transporter and CGRP mRNA expression than carriers of the ALDH2*2 allele. Therefore, we strongly suggested that GTN can stimulate CGRP expression by elevating the AEA transporter activity, which is affected by ALDH2 Glu504Lys polymorphism.

Authors+Show Affiliations

*Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China; †Institute of Clinical Pharmacology, Central South University, Changsha, China; and ‡Department of Pharmacology, School of Pharmaceutical Science, Central South University, Changsha, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25098345

Citation

Peng, Li-Ming, et al. "Involvement of Anandamide Transporter in Calcitonin Gene-related Peptide Expression Stimulated By Nitroglycerin and Influence of ALDH2 Glu504Lys Polymorphism." Journal of Cardiovascular Pharmacology, vol. 64, no. 5, 2014, pp. 460-4.
Peng LM, Chen XP, Shi RZ, et al. Involvement of anandamide transporter in calcitonin gene-related peptide expression stimulated by nitroglycerin and influence of ALDH2 Glu504Lys polymorphism. J Cardiovasc Pharmacol. 2014;64(5):460-4.
Peng, L. M., Chen, X. P., Shi, R. Z., Chen, L., Li, Y. J., & Yang, T. L. (2014). Involvement of anandamide transporter in calcitonin gene-related peptide expression stimulated by nitroglycerin and influence of ALDH2 Glu504Lys polymorphism. Journal of Cardiovascular Pharmacology, 64(5), 460-4. https://doi.org/10.1097/FJC.0000000000000138
Peng LM, et al. Involvement of Anandamide Transporter in Calcitonin Gene-related Peptide Expression Stimulated By Nitroglycerin and Influence of ALDH2 Glu504Lys Polymorphism. J Cardiovasc Pharmacol. 2014;64(5):460-4. PubMed PMID: 25098345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of anandamide transporter in calcitonin gene-related peptide expression stimulated by nitroglycerin and influence of ALDH2 Glu504Lys polymorphism. AU - Peng,Li-Ming, AU - Chen,Xiao-Ping, AU - Shi,Rui-Zheng, AU - Chen,Lei, AU - Li,Yuan-Jian, AU - Yang,Tian-Lun, PY - 2014/8/8/entrez PY - 2014/8/8/pubmed PY - 2015/7/15/medline SP - 460 EP - 4 JF - Journal of cardiovascular pharmacology JO - J Cardiovasc Pharmacol VL - 64 IS - 5 N2 - The aim of this study was to investigate whether N-arachidonic acid ethanolamine (anandamide, AEA) transporter contributed to calcitonin gene-related peptide (CGRP) expression mediated by nitroglycerin (GTN) in peripheral blood mononuclear cells (PBMCs) of healthy volunteers and its association with the mitochondrial aldehyde dehydrogenase-2 (ALDH2) Glu504Lys (ALDH2*2) polymorphism. In 10 ALDH2*2-genotyped Chinese volunteers, we assessed the activity of AEA transporter and expression of CGRP messenger ribonucleic acid (mRNA) in cultured PBMCs treated with different concentration of GTN with or without pretreatment with AM404 (the AEA transporter blocker). In this study, the activity of AEA transporter and expression of CGRP mRNA elevated with the increase in the concentration of GTN. Pretreatment of the cells with AM404 (1 μM) 2 hours before GTN reduced the GTN-induced increase in both AEA transporter activity and CGRP mRNA expression significantly, and cells with the ALDH2*1/*1 homozygote genotype showed significantly higher activity of AEA transporter and CGRP mRNA expression than carriers of the ALDH2*2 allele. Therefore, we strongly suggested that GTN can stimulate CGRP expression by elevating the AEA transporter activity, which is affected by ALDH2 Glu504Lys polymorphism. SN - 1533-4023 UR - https://www.unboundmedicine.com/medline/citation/25098345/Involvement_of_anandamide_transporter_in_calcitonin_gene_related_peptide_expression_stimulated_by_nitroglycerin_and_influence_of_ALDH2_Glu504Lys_polymorphism_ L2 - https://doi.org/10.1097/FJC.0000000000000138 DB - PRIME DP - Unbound Medicine ER -