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Expression of Xylella fastidiosa RpfF in citrus disrupts signaling in Xanthomonas citri subsp. citri and thereby its virulence.
Mol Plant Microbe Interact. 2014 Nov; 27(11):1241-52.MP

Abstract

Xylella fastidiosa and Xanthomonas citri subsp. citri, that cause citrus variegated chlorosis (CVC) and citrus canker diseases, respectively, utilize diffusible signal factor (DSF) for quorum sensing. DSF, produced by RpfF, are similar fatty acids in both organisms, although a different set of genes is regulated by DSF in each species. Because of this similarity, Xylella fastidiosa DSF might be recognized and affect the biology of Xanthomonas citri. Therefore, transgenic Citrus sinensis and Carrizo citrange plants overexpressing the Xylella fastidiosa rpfF were inoculated with Xanthomonas citri and changes in symptoms of citrus canker were observed. X. citri biofilms formed only at wound sites on transgenic leaves and were thicker; however, bacteria were unable to break through the tissue and form pustules elsewhere. Although abundant growth of X. citri occurred at wound sites on inoculated transgenic leaves, little growth was observed on unwounded tissue. Genes in the DFS-responsive core in X. citri were downregulated in bacteria isolated from transgenic leaves. DSF-dependent expression of engA was suppressed in cells exposed to xylem sap from transgenic plants. Thus, altered symptom development appears to be due to reduced expression of virulence genes because of the presence of antagonists of DSF signaling in X. citri in rpfF-expressing plants.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25099341

Citation

Caserta, R, et al. "Expression of Xylella Fastidiosa RpfF in Citrus Disrupts Signaling in Xanthomonas Citri Subsp. Citri and Thereby Its Virulence." Molecular Plant-microbe Interactions : MPMI, vol. 27, no. 11, 2014, pp. 1241-52.
Caserta R, Picchi SC, Takita MA, et al. Expression of Xylella fastidiosa RpfF in citrus disrupts signaling in Xanthomonas citri subsp. citri and thereby its virulence. Mol Plant Microbe Interact. 2014;27(11):1241-52.
Caserta, R., Picchi, S. C., Takita, M. A., Tomaz, J. P., Pereira, W. E., Machado, M. A., Ionescu, M., Lindow, S., & De Souza, A. A. (2014). Expression of Xylella fastidiosa RpfF in citrus disrupts signaling in Xanthomonas citri subsp. citri and thereby its virulence. Molecular Plant-microbe Interactions : MPMI, 27(11), 1241-52. https://doi.org/10.1094/MPMI-03-14-0090-R
Caserta R, et al. Expression of Xylella Fastidiosa RpfF in Citrus Disrupts Signaling in Xanthomonas Citri Subsp. Citri and Thereby Its Virulence. Mol Plant Microbe Interact. 2014;27(11):1241-52. PubMed PMID: 25099341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of Xylella fastidiosa RpfF in citrus disrupts signaling in Xanthomonas citri subsp. citri and thereby its virulence. AU - Caserta,R, AU - Picchi,S C, AU - Takita,M A, AU - Tomaz,J P, AU - Pereira,W E L, AU - Machado,M A, AU - Ionescu,M, AU - Lindow,S, AU - De Souza,A A, PY - 2014/8/8/entrez PY - 2014/8/8/pubmed PY - 2015/6/19/medline SP - 1241 EP - 52 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 27 IS - 11 N2 - Xylella fastidiosa and Xanthomonas citri subsp. citri, that cause citrus variegated chlorosis (CVC) and citrus canker diseases, respectively, utilize diffusible signal factor (DSF) for quorum sensing. DSF, produced by RpfF, are similar fatty acids in both organisms, although a different set of genes is regulated by DSF in each species. Because of this similarity, Xylella fastidiosa DSF might be recognized and affect the biology of Xanthomonas citri. Therefore, transgenic Citrus sinensis and Carrizo citrange plants overexpressing the Xylella fastidiosa rpfF were inoculated with Xanthomonas citri and changes in symptoms of citrus canker were observed. X. citri biofilms formed only at wound sites on transgenic leaves and were thicker; however, bacteria were unable to break through the tissue and form pustules elsewhere. Although abundant growth of X. citri occurred at wound sites on inoculated transgenic leaves, little growth was observed on unwounded tissue. Genes in the DFS-responsive core in X. citri were downregulated in bacteria isolated from transgenic leaves. DSF-dependent expression of engA was suppressed in cells exposed to xylem sap from transgenic plants. Thus, altered symptom development appears to be due to reduced expression of virulence genes because of the presence of antagonists of DSF signaling in X. citri in rpfF-expressing plants. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/25099341/Expression_of_Xylella_fastidiosa_RpfF_in_citrus_disrupts_signaling_in_Xanthomonas_citri_subsp__citri_and_thereby_its_virulence_ L2 - https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-14-0090-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -