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Patient-tailored application for Duchene muscular dystrophy on mdx mice based induced mesenchymal stem cells.
Exp Mol Pathol. 2014 Oct; 97(2):253-8.EM

Abstract

Mesenchymal stem cells (MSCs) may be used as powerful tools for the repair and regeneration of damaged tissues. However, isolating tissue specific-derived MSCs may cause pain and increased infection rates in patients, and repetitive isolations may be required. To overcome these difficulties, we have examined alternative methods for MSC production. Here, we show that induced pluripotent stem cells (iPSCs) may be differentiated into mesenchymal stem cells (iMSCs) following exposure to SB431542. Purified iMSCs were administered to mdx mice to study skeletal muscle regeneration in a murine model of muscular dystrophy. Purified iMSCs displayed fibroblast-like morphology, formed three-dimensional spheroid structures, and expressed characteristic mesenchymal stem cell surface markers such as CD29, CD33, CD73, CD90, and CD105. Moreover, iMSCs were capable of differentiating into adipogenic, osteogenic, and chondrogenic lineages. Transplanting iMSC cells to tibialis anterior skeletal muscle tissue in mdx mice lowered oxidative damage as evidenced by a reduction in nitrotyrosine levels, and normal dystrophin expression levels were restored. This study demonstrates the therapeutic potential of purified iMSCs in skeletal muscle regeneration in mdx mice, and suggests that iPSCs are a viable alternate source for deriving MSCs as needed.

Authors+Show Affiliations

Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, South Korea; BK21 Plus Project, Yonsei University College of Dentistry, Seoul 120-752, South Korea. Electronic address: jmj1103@yuhs.ac.Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, South Korea; Research Center for Radio-Senescence, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, South Korea.National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, South Korea.Department of Biomedical Science, College of Life Science, CHA University, Seoul 135-081, South Korea.Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, South Korea; Research Center for Radio-Senescence, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, South Korea. Electronic address: hck@kirams.re.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25102299

Citation

Jeong, Jaemin, et al. "Patient-tailored Application for Duchene Muscular Dystrophy On Mdx Mice Based Induced Mesenchymal Stem Cells." Experimental and Molecular Pathology, vol. 97, no. 2, 2014, pp. 253-8.
Jeong J, Shin K, Lee SB, et al. Patient-tailored application for Duchene muscular dystrophy on mdx mice based induced mesenchymal stem cells. Exp Mol Pathol. 2014;97(2):253-8.
Jeong, J., Shin, K., Lee, S. B., Lee, D. R., & Kwon, H. (2014). Patient-tailored application for Duchene muscular dystrophy on mdx mice based induced mesenchymal stem cells. Experimental and Molecular Pathology, 97(2), 253-8. https://doi.org/10.1016/j.yexmp.2014.08.001
Jeong J, et al. Patient-tailored Application for Duchene Muscular Dystrophy On Mdx Mice Based Induced Mesenchymal Stem Cells. Exp Mol Pathol. 2014;97(2):253-8. PubMed PMID: 25102299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Patient-tailored application for Duchene muscular dystrophy on mdx mice based induced mesenchymal stem cells. AU - Jeong,Jaemin, AU - Shin,Kyungshin, AU - Lee,Seung Bum, AU - Lee,Dong Ryul, AU - Kwon,Heechung, Y1 - 2014/08/04/ PY - 2014/04/28/received PY - 2014/08/01/accepted PY - 2014/8/8/entrez PY - 2014/8/8/pubmed PY - 2014/11/19/medline KW - Duchenne muscular dystrophy KW - Induced pluripotent stem cells KW - Mesenchymal stem cells KW - Regeneration KW - Skeletal muscle KW - Transplantation SP - 253 EP - 8 JF - Experimental and molecular pathology JO - Exp Mol Pathol VL - 97 IS - 2 N2 - Mesenchymal stem cells (MSCs) may be used as powerful tools for the repair and regeneration of damaged tissues. However, isolating tissue specific-derived MSCs may cause pain and increased infection rates in patients, and repetitive isolations may be required. To overcome these difficulties, we have examined alternative methods for MSC production. Here, we show that induced pluripotent stem cells (iPSCs) may be differentiated into mesenchymal stem cells (iMSCs) following exposure to SB431542. Purified iMSCs were administered to mdx mice to study skeletal muscle regeneration in a murine model of muscular dystrophy. Purified iMSCs displayed fibroblast-like morphology, formed three-dimensional spheroid structures, and expressed characteristic mesenchymal stem cell surface markers such as CD29, CD33, CD73, CD90, and CD105. Moreover, iMSCs were capable of differentiating into adipogenic, osteogenic, and chondrogenic lineages. Transplanting iMSC cells to tibialis anterior skeletal muscle tissue in mdx mice lowered oxidative damage as evidenced by a reduction in nitrotyrosine levels, and normal dystrophin expression levels were restored. This study demonstrates the therapeutic potential of purified iMSCs in skeletal muscle regeneration in mdx mice, and suggests that iPSCs are a viable alternate source for deriving MSCs as needed. SN - 1096-0945 UR - https://www.unboundmedicine.com/medline/citation/25102299/Patient_tailored_application_for_Duchene_muscular_dystrophy_on_mdx_mice_based_induced_mesenchymal_stem_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4800(14)00127-0 DB - PRIME DP - Unbound Medicine ER -