Tags

Type your tag names separated by a space and hit enter

Absence of endo-1,4-β-glucanase KOR1 alters the jasmonate-dependent defence response to Pseudomonas syringae in Arabidopsis.
J Plant Physiol. 2014 Oct 15; 171(16):1524-32.JP

Abstract

During plant-pathogen interactions, the plant cell wall forms part of active defence against invaders. In recent years, cell wall-editing enzymes, associated with growth and development, have been related to plant susceptibility or resistance. Our previous work identified a role for several tomato and Arabidopsis endo-1,4-β-glucanases (EGs) in plant-pathogen interactions. Here we studied the response of the Arabidopsis thaliana T-DNA insertion mutant lacking EG Korrigan1 (KOR1) infected with Pseudomonas syringae. KOR1 is predicted to be an EG which is thought to participate in cellulose biosynthesis. We found that kor1-1 plants were more susceptible to P. syringae, and displayed severe disease symptoms and enhanced bacterial growth if compared to Wassilewskija (Ws) wild-type plants. Hormonal and gene expression analyses revealed that the jasmonic acid (JA) pathway was activated more in kor1-1 plants with an increase in the JA-biosynthesis gene LOX3 and a greater accumulation of JA. Upon infection the accumulation of JA and JA-isoleucine (JA-Ile) was higher than in wild-type plants and increased the induction of LOX3 and the JA-responsive PDF1.2 gene. In addition, the increase of salicylic acid (SA) in healthy and infected kor1-1 may reflect the complex interaction between JA and SA, which results in the more susceptible phenotype displayed by the infected mutant plants. Callose deposition was enhanced in infected kor1-1 and an increase in pathogen-induced hydrogen peroxide took place. The susceptible phenotype displayed by KOR1-deficient plants was coronatine-independent. No significant changes were detected in the hormonal profile of the kor1-1 plants infected by coronatine-deficient P. syringae cmaA, which supports that absence of EG KOR1 alters per se the plant response to infection. We previously reported increased resistance of kor1-1 to B. cinerea, hence, the lack of this EG alters cell wall properties and plant responses in such a way that benefits P. syringae colonisation but restricts B. cinerea invasion.

Authors+Show Affiliations

Departamento de Bioquímica y Biología Molecular, Universidad de Valencia, IATA (CSIC), Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain. Electronic address: Jaime.lopez-cruz@uv.es.Departamento de Bioquímica y Biología Molecular, Universidad de Valencia, IATA (CSIC), Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain. Electronic address: ivan.finiti@uv.es.Laboratorio de Bioquímica y Biotecnología, Área de Fisiología Vegetal, Departamento de Ciencias Agrarias y del Medio Natural, ESTCE, Universitat Jaume I, 12071 Castellón, Spain. Electronic address: ecrespo@guest.uji.es.Departamento de Bioquímica y Biología Molecular, Universidad de Valencia, IATA (CSIC), Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain. Electronic address: oscres@alumni.uv.es.Laboratorio de Bioquímica y Biotecnología, Área de Fisiología Vegetal, Departamento de Ciencias Agrarias y del Medio Natural, ESTCE, Universitat Jaume I, 12071 Castellón, Spain. Electronic address: garciap@uji.es.Departamento de Bioquímica y Biología Molecular, Universidad de Valencia, IATA (CSIC), Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain. Electronic address: carmen.gonzalez@uv.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25108263

Citation

López-Cruz, Jaime, et al. "Absence of Endo-1,4-β-glucanase KOR1 Alters the Jasmonate-dependent Defence Response to Pseudomonas Syringae in Arabidopsis." Journal of Plant Physiology, vol. 171, no. 16, 2014, pp. 1524-32.
López-Cruz J, Finiti I, Fernández-Crespo E, et al. Absence of endo-1,4-β-glucanase KOR1 alters the jasmonate-dependent defence response to Pseudomonas syringae in Arabidopsis. J Plant Physiol. 2014;171(16):1524-32.
López-Cruz, J., Finiti, I., Fernández-Crespo, E., Crespo-Salvador, O., García-Agustín, P., & González-Bosch, C. (2014). Absence of endo-1,4-β-glucanase KOR1 alters the jasmonate-dependent defence response to Pseudomonas syringae in Arabidopsis. Journal of Plant Physiology, 171(16), 1524-32. https://doi.org/10.1016/j.jplph.2014.07.006
López-Cruz J, et al. Absence of Endo-1,4-β-glucanase KOR1 Alters the Jasmonate-dependent Defence Response to Pseudomonas Syringae in Arabidopsis. J Plant Physiol. 2014 Oct 15;171(16):1524-32. PubMed PMID: 25108263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absence of endo-1,4-β-glucanase KOR1 alters the jasmonate-dependent defence response to Pseudomonas syringae in Arabidopsis. AU - López-Cruz,Jaime, AU - Finiti,Ivan, AU - Fernández-Crespo,Emma, AU - Crespo-Salvador,Oscar, AU - García-Agustín,Pilar, AU - González-Bosch,Carmen, Y1 - 2014/07/23/ PY - 2014/03/03/received PY - 2014/06/20/revised PY - 2014/07/14/accepted PY - 2014/8/10/entrez PY - 2014/8/12/pubmed PY - 2015/4/18/medline KW - Arabidopsis KW - Cell wall KW - Endo-glucanases KW - Plant response KW - Pseudomonas syringae SP - 1524 EP - 32 JF - Journal of plant physiology JO - J Plant Physiol VL - 171 IS - 16 N2 - During plant-pathogen interactions, the plant cell wall forms part of active defence against invaders. In recent years, cell wall-editing enzymes, associated with growth and development, have been related to plant susceptibility or resistance. Our previous work identified a role for several tomato and Arabidopsis endo-1,4-β-glucanases (EGs) in plant-pathogen interactions. Here we studied the response of the Arabidopsis thaliana T-DNA insertion mutant lacking EG Korrigan1 (KOR1) infected with Pseudomonas syringae. KOR1 is predicted to be an EG which is thought to participate in cellulose biosynthesis. We found that kor1-1 plants were more susceptible to P. syringae, and displayed severe disease symptoms and enhanced bacterial growth if compared to Wassilewskija (Ws) wild-type plants. Hormonal and gene expression analyses revealed that the jasmonic acid (JA) pathway was activated more in kor1-1 plants with an increase in the JA-biosynthesis gene LOX3 and a greater accumulation of JA. Upon infection the accumulation of JA and JA-isoleucine (JA-Ile) was higher than in wild-type plants and increased the induction of LOX3 and the JA-responsive PDF1.2 gene. In addition, the increase of salicylic acid (SA) in healthy and infected kor1-1 may reflect the complex interaction between JA and SA, which results in the more susceptible phenotype displayed by the infected mutant plants. Callose deposition was enhanced in infected kor1-1 and an increase in pathogen-induced hydrogen peroxide took place. The susceptible phenotype displayed by KOR1-deficient plants was coronatine-independent. No significant changes were detected in the hormonal profile of the kor1-1 plants infected by coronatine-deficient P. syringae cmaA, which supports that absence of EG KOR1 alters per se the plant response to infection. We previously reported increased resistance of kor1-1 to B. cinerea, hence, the lack of this EG alters cell wall properties and plant responses in such a way that benefits P. syringae colonisation but restricts B. cinerea invasion. SN - 1618-1328 UR - https://www.unboundmedicine.com/medline/citation/25108263/Absence_of_endo_14_β_glucanase_KOR1_alters_the_jasmonate_dependent_defence_response_to_Pseudomonas_syringae_in_Arabidopsis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0176-1617(14)00187-4 DB - PRIME DP - Unbound Medicine ER -