Tags

Type your tag names separated by a space and hit enter

The blast resistance gene Pi54of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains.
PLoS One. 2014; 9(8):e104840.Plos

Abstract

The dominant rice blast resistance gene Pi54 cloned by map-based cloning approach from indica rice cultivar Tetep confers broad spectrum resistance to Magnaporthe oryzae. In this investigation, an orthologue of Pi54 designated as Pi54of was cloned from Oryza officinalis conferring high degree of resistance to M. oryzae and is functionally validated. We have also characterized the Pi54of protein and demonstrate its interaction with AVR-Pi54 protein. The Pi54of encoded ∼43 kDa small and unique cytoplasmic LRR family of disease resistance protein having unique Zinc finger domain overlapped with the leucine rich repeat regions. Pi54of showed Magnaporthe-induced expression. The phylogenetic and western blot analysis confirmed orthologous nature of Pi54 and Pi54of genes, whereas the identity of protein was confirmed through MALDI-TOF analysis. The in silico analysis showed that Pi54of is structurally more stable than other cloned Pi54 proteins. The molecular docking revealed that Pi54of protein interacts with AVR-Pi54 through novel non-LRR domains such as STI1 and RhoGEF. The STI1 and GEF domains which interact with AVR-Pi54 are also components of rice defensome complex. The Pi54of protein showed differential domain specificity while interacting with the AVR protein. Functional complementation revealed that Pi54of transferred in two rice lines belonging to indica and japonica background imparts enhanced resistance against three highly virulent strains of M. oryzae. In this study, for the first time, we demonstrated that a rice blast resistance gene Pi54of cloned from wild species of rice provides high degree of resistance to M. oryzae and might display different molecular mechanism involved in AVRPi54-Pi54of interaction.

Authors+Show Affiliations

National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India.National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India.National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25111047

Citation

Devanna, Navadagi B., et al. "The Blast Resistance Gene Pi54of Cloned From Oryza Officinalis Interacts With Avr-Pi54 Through Its Novel non-LRR Domains." PloS One, vol. 9, no. 8, 2014, pp. e104840.
Devanna NB, Vijayan J, Sharma TR. The blast resistance gene Pi54of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains. PLoS One. 2014;9(8):e104840.
Devanna, N. B., Vijayan, J., & Sharma, T. R. (2014). The blast resistance gene Pi54of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains. PloS One, 9(8), e104840. https://doi.org/10.1371/journal.pone.0104840
Devanna NB, Vijayan J, Sharma TR. The Blast Resistance Gene Pi54of Cloned From Oryza Officinalis Interacts With Avr-Pi54 Through Its Novel non-LRR Domains. PLoS One. 2014;9(8):e104840. PubMed PMID: 25111047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The blast resistance gene Pi54of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains. AU - Devanna,Navadagi B, AU - Vijayan,Joshitha, AU - Sharma,Tilak R, Y1 - 2014/08/11/ PY - 2014/04/16/received PY - 2014/07/15/accepted PY - 2014/8/12/entrez PY - 2014/8/12/pubmed PY - 2016/2/5/medline SP - e104840 EP - e104840 JF - PloS one JO - PLoS One VL - 9 IS - 8 N2 - The dominant rice blast resistance gene Pi54 cloned by map-based cloning approach from indica rice cultivar Tetep confers broad spectrum resistance to Magnaporthe oryzae. In this investigation, an orthologue of Pi54 designated as Pi54of was cloned from Oryza officinalis conferring high degree of resistance to M. oryzae and is functionally validated. We have also characterized the Pi54of protein and demonstrate its interaction with AVR-Pi54 protein. The Pi54of encoded ∼43 kDa small and unique cytoplasmic LRR family of disease resistance protein having unique Zinc finger domain overlapped with the leucine rich repeat regions. Pi54of showed Magnaporthe-induced expression. The phylogenetic and western blot analysis confirmed orthologous nature of Pi54 and Pi54of genes, whereas the identity of protein was confirmed through MALDI-TOF analysis. The in silico analysis showed that Pi54of is structurally more stable than other cloned Pi54 proteins. The molecular docking revealed that Pi54of protein interacts with AVR-Pi54 through novel non-LRR domains such as STI1 and RhoGEF. The STI1 and GEF domains which interact with AVR-Pi54 are also components of rice defensome complex. The Pi54of protein showed differential domain specificity while interacting with the AVR protein. Functional complementation revealed that Pi54of transferred in two rice lines belonging to indica and japonica background imparts enhanced resistance against three highly virulent strains of M. oryzae. In this study, for the first time, we demonstrated that a rice blast resistance gene Pi54of cloned from wild species of rice provides high degree of resistance to M. oryzae and might display different molecular mechanism involved in AVRPi54-Pi54of interaction. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25111047/The_blast_resistance_gene_Pi54of_cloned_from_Oryza_officinalis_interacts_with_Avr_Pi54_through_its_novel_non_LRR_domains_ DB - PRIME DP - Unbound Medicine ER -