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mecA-positive methicillin-sensitive Staphylococcus aureus clinical isolates in Zenica-Doboj Canton, Bosnia and Herzegovina.
J Chemother. 2015; 27(6):330-6.JC

Abstract

Forty-four mecA-positive and eight mecA-negative Staphylococcus aureus isolates confirmed by PCR were further tested by disc-diffusion (DD) oxacillin and cefoxitin, oxacillin Epsilon (E)-test, and oxacillin and cefoxitin minimal inhibitory concentration (MIC) Strip methicillin-resistant phenotype in S. aureus (MRSA) tests. Among 44 mecA-positive S. aureus isolates, two (4·5%) were detected as MRSA by DD-oxacillin, 17 (38·6%) by DD-cefoxitin test, and seven (15·9%) by the E-test. In the cefoxitin MIC Strip MRSA test, 19 (43·2%) isolates were resistant. In the oxacillin MIC Strip MRSA test, 18 (40·9%) isolates were resistant and 26 (59·1%) were sensitive, i.e. oxacillin-sensitive MRSA (OS-MRSA) (MIC range 0·25-≤0·25 mg/l). Fifteen out of 26 OS-MRSA (57·7%) belonged to spa-CC 355/595, 78% of which belonged to the largest PFGE clone. Some discrepancies between the phenotypic methods for MRSA identification obtained in this study were caused by large proportion of OS-MRSA. Misidentification of OS-MRSA as MSSA might result in an appearance of highly resistant MRSA in patients treated with beta-lactam antibiotics.

Authors+Show Affiliations

University of Ljubljana, Biotechnical Faculty, Microbiology Department , Ljubljana, Slovenia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25112955

Citation

Kamberović, Farah, et al. "MecA-positive Methicillin-sensitive Staphylococcus Aureus Clinical Isolates in Zenica-Doboj Canton, Bosnia and Herzegovina." Journal of Chemotherapy (Florence, Italy), vol. 27, no. 6, 2015, pp. 330-6.
Kamberović F, Ibrahimagić A, Uzunović S, et al. MecA-positive methicillin-sensitive Staphylococcus aureus clinical isolates in Zenica-Doboj Canton, Bosnia and Herzegovina. J Chemother. 2015;27(6):330-6.
Kamberović, F., Ibrahimagić, A., Uzunović, S., Budimir, A., Rijnders, M. I., & Stobberingh, E. E. (2015). MecA-positive methicillin-sensitive Staphylococcus aureus clinical isolates in Zenica-Doboj Canton, Bosnia and Herzegovina. Journal of Chemotherapy (Florence, Italy), 27(6), 330-6. https://doi.org/10.1179/1973947814Y.0000000207
Kamberović F, et al. MecA-positive Methicillin-sensitive Staphylococcus Aureus Clinical Isolates in Zenica-Doboj Canton, Bosnia and Herzegovina. J Chemother. 2015;27(6):330-6. PubMed PMID: 25112955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - mecA-positive methicillin-sensitive Staphylococcus aureus clinical isolates in Zenica-Doboj Canton, Bosnia and Herzegovina. AU - Kamberović,Farah, AU - Ibrahimagić,Amir, AU - Uzunović,Selma, AU - Budimir,Ana, AU - Rijnders,Michelle I A, AU - Stobberingh,Ellen E, Y1 - 2014/08/11/ PY - 2014/8/13/entrez PY - 2014/8/13/pubmed PY - 2016/11/1/medline KW - Antimicrobial resistance KW - MRSA KW - spa-CC SP - 330 EP - 6 JF - Journal of chemotherapy (Florence, Italy) JO - J Chemother VL - 27 IS - 6 N2 - Forty-four mecA-positive and eight mecA-negative Staphylococcus aureus isolates confirmed by PCR were further tested by disc-diffusion (DD) oxacillin and cefoxitin, oxacillin Epsilon (E)-test, and oxacillin and cefoxitin minimal inhibitory concentration (MIC) Strip methicillin-resistant phenotype in S. aureus (MRSA) tests. Among 44 mecA-positive S. aureus isolates, two (4·5%) were detected as MRSA by DD-oxacillin, 17 (38·6%) by DD-cefoxitin test, and seven (15·9%) by the E-test. In the cefoxitin MIC Strip MRSA test, 19 (43·2%) isolates were resistant. In the oxacillin MIC Strip MRSA test, 18 (40·9%) isolates were resistant and 26 (59·1%) were sensitive, i.e. oxacillin-sensitive MRSA (OS-MRSA) (MIC range 0·25-≤0·25 mg/l). Fifteen out of 26 OS-MRSA (57·7%) belonged to spa-CC 355/595, 78% of which belonged to the largest PFGE clone. Some discrepancies between the phenotypic methods for MRSA identification obtained in this study were caused by large proportion of OS-MRSA. Misidentification of OS-MRSA as MSSA might result in an appearance of highly resistant MRSA in patients treated with beta-lactam antibiotics. SN - 1973-9478 UR - https://www.unboundmedicine.com/medline/citation/25112955/mecA_positive_methicillin_sensitive_Staphylococcus_aureus_clinical_isolates_in_Zenica_Doboj_Canton_Bosnia_and_Herzegovina_ L2 - http://www.tandfonline.com/doi/full/10.1179/1973947814Y.0000000207 DB - PRIME DP - Unbound Medicine ER -