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Overlapped sequence types (STs) and serogroups of avian pathogenic (APEC) and human extra-intestinal pathogenic (ExPEC) Escherichia coli isolated in Brazil.
PLoS One. 2014; 9(8):e105016.Plos

Abstract

Avian pathogenic Escherichia coli (APEC) strains belong to a category that is associated with colibacillosis, a serious illness in the poultry industry worldwide. Additionally, some APEC groups have recently been described as potential zoonotic agents. In this work, we compared APEC strains with extraintestinal pathogenic E. coli (ExPEC) strains isolated from clinical cases of humans with extra-intestinal diseases such as urinary tract infections (UTI) and bacteremia. PCR results showed that genes usually found in the ColV plasmid (tsh, iucA, iss, and hlyF) were associated with APEC strains while fyuA, irp-2, fepC sitDchrom, fimH, crl, csgA, afa, iha, sat, hlyA, hra, cnf1, kpsMTII, clpVSakai and malX were associated with human ExPEC. Both categories shared nine serogroups (O2, O6, O7, O8, O11, O19, O25, O73 and O153) and seven sequence types (ST10, ST88, ST93, ST117, ST131, ST155, ST359, ST648 and ST1011). Interestingly, ST95, which is associated with the zoonotic potential of APEC and is spread in avian E. coli of North America and Europe, was not detected among 76 APEC strains. When the strains were clustered based on the presence of virulence genes, most ExPEC strains (71.7%) were contained in one cluster while most APEC strains (63.2%) segregated to another. In general, the strains showed distinct genetic and fingerprint patterns, but avian and human strains of ST359, or ST23 clonal complex (CC), presented more than 70% of similarity by PFGE. The results demonstrate that some "zoonotic-related" STs (ST117, ST131, ST10CC, ST23CC) are present in Brazil. Also, the presence of moderate fingerprint similarities between ST359 E. coli of avian and human origin indicates that strains of this ST are candidates for having zoonotic potential.

Authors+Show Affiliations

Bacterial Molecular Biology Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.Instituto Adolfo Lutz, São Paulo, SP, Brazil.Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.Instituto Biológico, CAPTAA, Unidade de Pesquisa e Desenvolvimento de Bastos, Bastos, SP, Brazil.Bacterial Molecular Biology Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.Departamento de Clínica Médica, Faculdade de Medicina, Universidade Estadual Paulista, Botucatu, SP, Brazil.Departamento de Microbiologia e Imunologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brazil.Department of Internal Medicine, UNICAMP, Campinas, Brazil.Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.Bacterial Molecular Biology Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25115913

Citation

Maluta, Renato Pariz, et al. "Overlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-intestinal Pathogenic (ExPEC) Escherichia Coli Isolated in Brazil." PloS One, vol. 9, no. 8, 2014, pp. e105016.
Maluta RP, Logue CM, Casas MR, et al. Overlapped sequence types (STs) and serogroups of avian pathogenic (APEC) and human extra-intestinal pathogenic (ExPEC) Escherichia coli isolated in Brazil. PLoS One. 2014;9(8):e105016.
Maluta, R. P., Logue, C. M., Casas, M. R., Meng, T., Guastalli, E. A., Rojas, T. C., Montelli, A. C., Sadatsune, T., de Carvalho Ramos, M., Nolan, L. K., & da Silveira, W. D. (2014). Overlapped sequence types (STs) and serogroups of avian pathogenic (APEC) and human extra-intestinal pathogenic (ExPEC) Escherichia coli isolated in Brazil. PloS One, 9(8), e105016. https://doi.org/10.1371/journal.pone.0105016
Maluta RP, et al. Overlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-intestinal Pathogenic (ExPEC) Escherichia Coli Isolated in Brazil. PLoS One. 2014;9(8):e105016. PubMed PMID: 25115913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overlapped sequence types (STs) and serogroups of avian pathogenic (APEC) and human extra-intestinal pathogenic (ExPEC) Escherichia coli isolated in Brazil. AU - Maluta,Renato Pariz, AU - Logue,Catherine Mary, AU - Casas,Monique Ribeiro Tiba, AU - Meng,Ting, AU - Guastalli,Elisabete Aparecida Lopes, AU - Rojas,Thaís Cabrera Galvão, AU - Montelli,Augusto Cezar, AU - Sadatsune,Teruê, AU - de Carvalho Ramos,Marcelo, AU - Nolan,Lisa Kay, AU - da Silveira,Wanderley Dias, Y1 - 2014/08/12/ PY - 2014/05/13/received PY - 2014/07/17/accepted PY - 2014/8/14/entrez PY - 2014/8/15/pubmed PY - 2016/4/19/medline SP - e105016 EP - e105016 JF - PloS one JO - PLoS One VL - 9 IS - 8 N2 - Avian pathogenic Escherichia coli (APEC) strains belong to a category that is associated with colibacillosis, a serious illness in the poultry industry worldwide. Additionally, some APEC groups have recently been described as potential zoonotic agents. In this work, we compared APEC strains with extraintestinal pathogenic E. coli (ExPEC) strains isolated from clinical cases of humans with extra-intestinal diseases such as urinary tract infections (UTI) and bacteremia. PCR results showed that genes usually found in the ColV plasmid (tsh, iucA, iss, and hlyF) were associated with APEC strains while fyuA, irp-2, fepC sitDchrom, fimH, crl, csgA, afa, iha, sat, hlyA, hra, cnf1, kpsMTII, clpVSakai and malX were associated with human ExPEC. Both categories shared nine serogroups (O2, O6, O7, O8, O11, O19, O25, O73 and O153) and seven sequence types (ST10, ST88, ST93, ST117, ST131, ST155, ST359, ST648 and ST1011). Interestingly, ST95, which is associated with the zoonotic potential of APEC and is spread in avian E. coli of North America and Europe, was not detected among 76 APEC strains. When the strains were clustered based on the presence of virulence genes, most ExPEC strains (71.7%) were contained in one cluster while most APEC strains (63.2%) segregated to another. In general, the strains showed distinct genetic and fingerprint patterns, but avian and human strains of ST359, or ST23 clonal complex (CC), presented more than 70% of similarity by PFGE. The results demonstrate that some "zoonotic-related" STs (ST117, ST131, ST10CC, ST23CC) are present in Brazil. Also, the presence of moderate fingerprint similarities between ST359 E. coli of avian and human origin indicates that strains of this ST are candidates for having zoonotic potential. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25115913/Overlapped_sequence_types__STs__and_serogroups_of_avian_pathogenic__APEC__and_human_extra_intestinal_pathogenic__ExPEC__Escherichia_coli_isolated_in_Brazil_ L2 - https://dx.plos.org/10.1371/journal.pone.0105016 DB - PRIME DP - Unbound Medicine ER -