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Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis.
FASEB J. 2014 Nov; 28(11):4748-63.FJ

Abstract

In the present bed rest (BR) study, 23 volunteers were randomized into 3 subgroups: 60 d BR control (Ctr); BR with resistive exercise (RE; lower-limb load); and resistive vibration exercise (RVE; RE with superimposed vibration). The aim was to analyze by confocal and electron microscopy the effects of vibration on myofibril and filament integrity in soleus (Sol) and vastus lateralis (VL) muscle; differential proteomics of contractile, cytoskeletal, and costameric proteins (TN-C, ROCK1, and FAK); and expression of PGC1α and atrophy-related master genes MuRF1 and MuRF2. RVE (but not RE) preserved myofiber size and phenotype in Sol and VL by overexpressing MYBPC1 (42%, P ≤ 0.01), WDR1 (39%, P ≤ 0.01), sarcosin (84%, P ≤ 0.01), and CKM (20%, P ≤ 0.01) and prevented myofibrillar ultrastructural damage as detectable by MuRF1 expression. In Sol, cytoskeletal and contractile proteins were normalized by RVE, and TN-C increased (59%, P ≤ 0.01); the latter also with RE (108%, P ≤ 0.01). In VL, the outcomes of both RVE (acting on sarcosin and desmin) and RE (by way of troponinT-slow and MYL2) were similar. RVE appears to be a highly efficient countermeasure protocol against muscle atrophy and ultrastructural and molecular dysregulation induced by chronic disuse.

Authors+Show Affiliations

Center of Space Medicine Berlin, Neuromuscular Group, Institute of Anatomy, and michele.salanova@charite.de cecilia.gelfi@unimi.it.Department of Biomedical Sciences for Health, University of Milan, Milan, Italy; Consiglio Nazionale delle Ricerche (CNR), Institute of Bioimaging and Molecular Physiology, Segrate, Italy; Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Donato, San Donato Milanese, Italy; michele.salanova@charite.de cecilia.gelfi@unimi.it.Department of Biomedical Sciences for Health, University of Milan, Milan, Italy;Consiglio Nazionale delle Ricerche (CNR), Institute of Bioimaging and Molecular Physiology, Segrate, Italy; Institute of Bioimaging and Molecular Physiology, CNR-Laboratorio di Tecnologie Oncologiche (LATO), Cefalù, Italy; and.Department of Biomedical Sciences for Health, University of Milan, Milan, Italy;Institute of Bioimaging and Molecular Physiology, CNR-Laboratorio di Tecnologie Oncologiche (LATO), Cefalù, Italy; and.Department of Pharmacology and Toxicology, University of Lausanne, Lausanne, Switzerland.Center of Space Medicine Berlin, Neuromuscular Group, Institute of Anatomy, and.Center of Space Medicine Berlin, Neuromuscular Group, Institute of Anatomy, and.Center for Muscle and Bone Research (ZMK), Charité Universitätsmedizin Berlin, Berlin, Germany;Consiglio Nazionale delle Ricerche (CNR), Institute of Bioimaging and Molecular Physiology, Segrate, Italy;Center of Space Medicine Berlin, Neuromuscular Group, Institute of Anatomy, and.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25122557

Citation

Salanova, Michele, et al. "Disuse Deterioration of Human Skeletal Muscle Challenged By Resistive Exercise Superimposed With Vibration: Evidence From Structural and Proteomic Analysis." FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol. 28, no. 11, 2014, pp. 4748-63.
Salanova M, Gelfi C, Moriggi M, et al. Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis. FASEB J. 2014;28(11):4748-63.
Salanova, M., Gelfi, C., Moriggi, M., Vasso, M., Viganò, A., Minafra, L., Bonifacio, G., Schiffl, G., Gutsmann, M., Felsenberg, D., Cerretelli, P., & Blottner, D. (2014). Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 28(11), 4748-63. https://doi.org/10.1096/fj.14-252825
Salanova M, et al. Disuse Deterioration of Human Skeletal Muscle Challenged By Resistive Exercise Superimposed With Vibration: Evidence From Structural and Proteomic Analysis. FASEB J. 2014;28(11):4748-63. PubMed PMID: 25122557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis. AU - Salanova,Michele, AU - Gelfi,Cecilia, AU - Moriggi,Manuela, AU - Vasso,Michele, AU - Viganò,Agnese, AU - Minafra,Luigi, AU - Bonifacio,Gaetano, AU - Schiffl,Gudrun, AU - Gutsmann,Martina, AU - Felsenberg,Dieter, AU - Cerretelli,Paolo, AU - Blottner,Dieter, Y1 - 2014/08/13/ PY - 2014/8/15/entrez PY - 2014/8/15/pubmed PY - 2015/1/28/medline KW - atrophy KW - bed rest KW - countermeasures KW - protein expression KW - ultrastructure SP - 4748 EP - 63 JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JO - FASEB J VL - 28 IS - 11 N2 - In the present bed rest (BR) study, 23 volunteers were randomized into 3 subgroups: 60 d BR control (Ctr); BR with resistive exercise (RE; lower-limb load); and resistive vibration exercise (RVE; RE with superimposed vibration). The aim was to analyze by confocal and electron microscopy the effects of vibration on myofibril and filament integrity in soleus (Sol) and vastus lateralis (VL) muscle; differential proteomics of contractile, cytoskeletal, and costameric proteins (TN-C, ROCK1, and FAK); and expression of PGC1α and atrophy-related master genes MuRF1 and MuRF2. RVE (but not RE) preserved myofiber size and phenotype in Sol and VL by overexpressing MYBPC1 (42%, P ≤ 0.01), WDR1 (39%, P ≤ 0.01), sarcosin (84%, P ≤ 0.01), and CKM (20%, P ≤ 0.01) and prevented myofibrillar ultrastructural damage as detectable by MuRF1 expression. In Sol, cytoskeletal and contractile proteins were normalized by RVE, and TN-C increased (59%, P ≤ 0.01); the latter also with RE (108%, P ≤ 0.01). In VL, the outcomes of both RVE (acting on sarcosin and desmin) and RE (by way of troponinT-slow and MYL2) were similar. RVE appears to be a highly efficient countermeasure protocol against muscle atrophy and ultrastructural and molecular dysregulation induced by chronic disuse. SN - 1530-6860 UR - https://www.unboundmedicine.com/medline/citation/25122557/Disuse_deterioration_of_human_skeletal_muscle_challenged_by_resistive_exercise_superimposed_with_vibration:_evidence_from_structural_and_proteomic_analysis_ L2 - https://doi.org/10.1096/fj.14-252825 DB - PRIME DP - Unbound Medicine ER -