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Identification of promoter polymorphisms in the cytochrome P450 CYP6AY1 linked with insecticide resistance in the brown planthopper, Nilaparvata lugens.
Insect Mol Biol. 2014 Dec; 23(6):768-78.IM

Abstract

Imidacloprid resistance in the brown planthopper, Nilaparvata lugens, is primarily the result of the over-expression of cytochrome P450 monooxygenases. Here, a field-collected strain of N. lugens was shown to be highly resistant to both imidacloprid and buprofezin. Insecticide exposure and quantitative real-time PCR revealed that its resistance was mainly associated with a cytochrome P450 gene, CYP6AY1. CYP6AY1 is known to metabolize imidacloprid but its effect on buprofezin is unclear. In the 5'-untranslated region of CYP6AY1, a novel alternative splicing was detected. After a 1990-bp promoter region was cloned, its basal luciferase activity was assessed. Furthermore, genotyping studies identified 12 variations in the promoter region that discriminated between the field-collected and control strain. Finally, survival bioassays revealed a single nucleotide polymorphism and an insertion-deletion polymorphism linked to buprofezin and imidacloprid resistance. Mutagenesis of these sites enhanced the promoter activity of CYP6AY1. These results suggest that promoter polymorphisms may affect P450-mediated multiple insecticide resistance of pests.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Pang R
Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China.
Li Y
No affiliation info available
Dong Y
No affiliation info available
Liang Z
No affiliation info available
Zhang Y
No affiliation info available
Zhang W
No affiliation info available

MeSH

AnimalsChinaCytochrome P-450 Enzyme SystemHemipteraImidazolesInsecticide ResistanceInsecticidesLuciferasesMutagenesis, Site-DirectedNeonicotinoidsNitro CompoundsPolymorphism, GeneticPromoter Regions, GeneticReal-Time Polymerase Chain ReactionThiadiazines

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25124988

Citation

Pang, R, et al. "Identification of Promoter Polymorphisms in the Cytochrome P450 CYP6AY1 Linked With Insecticide Resistance in the Brown Planthopper, Nilaparvata Lugens." Insect Molecular Biology, vol. 23, no. 6, 2014, pp. 768-78.
Pang R, Li Y, Dong Y, et al. Identification of promoter polymorphisms in the cytochrome P450 CYP6AY1 linked with insecticide resistance in the brown planthopper, Nilaparvata lugens. Insect Mol Biol. 2014;23(6):768-78.
Pang, R., Li, Y., Dong, Y., Liang, Z., Zhang, Y., & Zhang, W. (2014). Identification of promoter polymorphisms in the cytochrome P450 CYP6AY1 linked with insecticide resistance in the brown planthopper, Nilaparvata lugens. Insect Molecular Biology, 23(6), 768-78. https://doi.org/10.1111/imb.12121
Pang R, et al. Identification of Promoter Polymorphisms in the Cytochrome P450 CYP6AY1 Linked With Insecticide Resistance in the Brown Planthopper, Nilaparvata Lugens. Insect Mol Biol. 2014;23(6):768-78. PubMed PMID: 25124988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of promoter polymorphisms in the cytochrome P450 CYP6AY1 linked with insecticide resistance in the brown planthopper, Nilaparvata lugens. AU - Pang,R, AU - Li,Y, AU - Dong,Y, AU - Liang,Z, AU - Zhang,Y, AU - Zhang,W, Y1 - 2014/08/14/ PY - 2014/8/16/entrez PY - 2014/8/16/pubmed PY - 2015/7/24/medline KW - P450 KW - buprofezin KW - imidacloprid KW - promoter polymorphisms SP - 768 EP - 78 JF - Insect molecular biology JO - Insect Mol Biol VL - 23 IS - 6 N2 - Imidacloprid resistance in the brown planthopper, Nilaparvata lugens, is primarily the result of the over-expression of cytochrome P450 monooxygenases. Here, a field-collected strain of N. lugens was shown to be highly resistant to both imidacloprid and buprofezin. Insecticide exposure and quantitative real-time PCR revealed that its resistance was mainly associated with a cytochrome P450 gene, CYP6AY1. CYP6AY1 is known to metabolize imidacloprid but its effect on buprofezin is unclear. In the 5'-untranslated region of CYP6AY1, a novel alternative splicing was detected. After a 1990-bp promoter region was cloned, its basal luciferase activity was assessed. Furthermore, genotyping studies identified 12 variations in the promoter region that discriminated between the field-collected and control strain. Finally, survival bioassays revealed a single nucleotide polymorphism and an insertion-deletion polymorphism linked to buprofezin and imidacloprid resistance. Mutagenesis of these sites enhanced the promoter activity of CYP6AY1. These results suggest that promoter polymorphisms may affect P450-mediated multiple insecticide resistance of pests. SN - 1365-2583 UR - https://www.unboundmedicine.com/medline/citation/25124988/Identification_of_promoter_polymorphisms_in_the_cytochrome_P450_CYP6AY1_linked_with_insecticide_resistance_in_the_brown_planthopper_Nilaparvata_lugens_ DB - PRIME DP - Unbound Medicine ER -