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Identification of putative kdr mutations in the tropical bed bug, Cimex hemipterus (Hemiptera: Cimicidae).
Pest Manag Sci. 2015 Jul; 71(7):1015-20.PM

Abstract

BACKGROUND

Bed bugs [both Cimex hemipterus (F.) and Cimex lectularius L.] are highly resistant to pyrethroids worldwide. An important resistance mechanism known as 'knockdown resistance' (kdr) is caused by genetic point mutations on the voltage-gated sodium channel (VGSC) gene. Previous studies have identified two point mutations (V419L and L925I) on the VGSC gene in C. lectularius that are responsible for kdr-type resistance. However, the kdr mutations in C. hemipterus have not been investigated.

RESULTS

Four novel mutations, L899V (leucine to valine), M918I (methionine to isoleucine), D953G (aspartic acid to glycine) and L1014F (leucine to phenylalanine), were identified in the domain II region of the C. hemipterus VGSC gene. This region has been widely investigated for the study of kdr-type resistance to pyrethroids in other insect pests. The V419L and L925I kdr mutations as previously identified in C. lectularius were not detected in C. hemipterus.

CONCLUSION

M918I and L1014F are considered to be probable kdr mutations and may play essential roles in kdr-type resistance to pyrethroids in C. hemipterus. Further studies are under way in the authors' laboratory to determine the non-kdr-type resistance mechanisms in C. hemipterus.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Dang K
Department of Medical Entomology, Institute for Clinical Pathology and Medical Research, Westmead Hospital, Westmead, NSW, Australia. Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China.
Toi CS
Department of Medical Entomology, Institute for Clinical Pathology and Medical Research, Westmead Hospital, Westmead, NSW, Australia.
Lilly DG
Department of Medical Entomology, Institute for Clinical Pathology and Medical Research, Westmead Hospital, Westmead, NSW, Australia.
Lee CY
Urban Entomology Laboratory, Vector Control Research Unit, School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia.
Naylor R
Prior's Loft, Coleford Road, Tidenham, Monmouthshire, UK.
Tawatsin A
Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
Thavara U
Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
Bu W
Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China.
Doggett SL
Department of Medical Entomology, Institute for Clinical Pathology and Medical Research, Westmead Hospital, Westmead, NSW, Australia.

MeSH

AllethrinsAnimalsBedbugsInsecticide ResistanceInsecticidesPoint MutationPyrethrinsVoltage-Gated Sodium Channels

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25132449

Citation

Dang, Kai, et al. "Identification of Putative Kdr Mutations in the Tropical Bed Bug, Cimex Hemipterus (Hemiptera: Cimicidae)." Pest Management Science, vol. 71, no. 7, 2015, pp. 1015-20.
Dang K, Toi CS, Lilly DG, et al. Identification of putative kdr mutations in the tropical bed bug, Cimex hemipterus (Hemiptera: Cimicidae). Pest Manag Sci. 2015;71(7):1015-20.
Dang, K., Toi, C. S., Lilly, D. G., Lee, C. Y., Naylor, R., Tawatsin, A., Thavara, U., Bu, W., & Doggett, S. L. (2015). Identification of putative kdr mutations in the tropical bed bug, Cimex hemipterus (Hemiptera: Cimicidae). Pest Management Science, 71(7), 1015-20. https://doi.org/10.1002/ps.3880
Dang K, et al. Identification of Putative Kdr Mutations in the Tropical Bed Bug, Cimex Hemipterus (Hemiptera: Cimicidae). Pest Manag Sci. 2015;71(7):1015-20. PubMed PMID: 25132449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of putative kdr mutations in the tropical bed bug, Cimex hemipterus (Hemiptera: Cimicidae). AU - Dang,Kai, AU - Toi,Cheryl S, AU - Lilly,David G, AU - Lee,Chow-Yang, AU - Naylor,Richard, AU - Tawatsin,Apiwat, AU - Thavara,Usavadee, AU - Bu,Wenun, AU - Doggett,Stephen L, Y1 - 2014/09/11/ PY - 2014/05/20/received PY - 2014/08/10/revised PY - 2014/08/10/accepted PY - 2014/8/19/entrez PY - 2014/8/19/pubmed PY - 2016/5/4/medline KW - Cimex KW - insecticide resistance KW - knockdown resistance KW - sodium channel SP - 1015 EP - 20 JF - Pest management science JO - Pest Manag Sci VL - 71 IS - 7 N2 - BACKGROUND: Bed bugs [both Cimex hemipterus (F.) and Cimex lectularius L.] are highly resistant to pyrethroids worldwide. An important resistance mechanism known as 'knockdown resistance' (kdr) is caused by genetic point mutations on the voltage-gated sodium channel (VGSC) gene. Previous studies have identified two point mutations (V419L and L925I) on the VGSC gene in C. lectularius that are responsible for kdr-type resistance. However, the kdr mutations in C. hemipterus have not been investigated. RESULTS: Four novel mutations, L899V (leucine to valine), M918I (methionine to isoleucine), D953G (aspartic acid to glycine) and L1014F (leucine to phenylalanine), were identified in the domain II region of the C. hemipterus VGSC gene. This region has been widely investigated for the study of kdr-type resistance to pyrethroids in other insect pests. The V419L and L925I kdr mutations as previously identified in C. lectularius were not detected in C. hemipterus. CONCLUSION: M918I and L1014F are considered to be probable kdr mutations and may play essential roles in kdr-type resistance to pyrethroids in C. hemipterus. Further studies are under way in the authors' laboratory to determine the non-kdr-type resistance mechanisms in C. hemipterus. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/25132449/Identification_of_putative_kdr_mutations_in_the_tropical_bed_bug_Cimex_hemipterus__Hemiptera:_Cimicidae__ DB - PRIME DP - Unbound Medicine ER -