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Development of a rapid capture-cum-detection method for Escherichia coli O157 from apple juice comprising nano-immunomagnetic separation in tandem with surface enhanced Raman scattering.
Int J Food Microbiol. 2014 Oct 17; 189:89-97.IJ

Abstract

A combined capture and detection method comprising of nano-immunomagnetic separation (NIMS) and surface enhanced Raman spectroscopy (SERS) was developed to detect Escherichia coli O157 from liquid media including apple juice. The capture antibodies (cAbs) were immobilized on magnetite-gold (Fe3O4/Au) magnetic nanoparticles (MNPs) which were used for separation and concentration of the E. coli O157 cells from model liquid food matrix. The capture efficiency (CE) for E. coli O157 using MNP was found to be approximately 84-94%. No cross reactivity was observed with background non-target organisms. There was a significant difference in the mean CE of bacteria captured by MNP and commercially sourced immunomagnetic microbeads (p<0.05). For the detection of target pathogen, SERS labels were prepared by conjugating gold nanoparticles with Raman reporter molecules and the detector antibody (dAb). Au-Raman label-dAb was interacted with gold coated MNP-cAb-E. coli O157 complex. The ability of this immunoassay to detect E. coli O157 in apple juice was investigated. We have successfully applied the synthesized Fe3O4/Au nanoclusters to E. coli O157 detection in apple juice using the SERS method. The lowest detectable bacterial cell concentration in apple juice was 10(2)CFU/mL with a total analysis time of less than an hour. This method presents a convenient way of preconcentration, separation, and detection of low levels of target pathogen from liquid food matrix.

Authors+Show Affiliations

Laboratory of Food Microbiology and Immunochemistry, Department of Food & Animal Sciences, Alabama A&M University, Huntsville, AL 35762, USA.HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.Department of Physics, Alabama A&M University, Huntsville, AL 35762, USA.Division of Epidemiology, Biostatistics, and Environmental Health, School of Public Health, The University of Memphis, Memphis, TN 38152, USA; Laboratory of Food Microbiology and Immunochemistry, Department of Food & Animal Sciences, Alabama A&M University, Huntsville, AL 35762, USA. Electronic address: pbnerjee@memphis.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25133877

Citation

Najafi, Roya, et al. "Development of a Rapid Capture-cum-detection Method for Escherichia Coli O157 From Apple Juice Comprising Nano-immunomagnetic Separation in Tandem With Surface Enhanced Raman Scattering." International Journal of Food Microbiology, vol. 189, 2014, pp. 89-97.
Najafi R, Mukherjee S, Hudson J, et al. Development of a rapid capture-cum-detection method for Escherichia coli O157 from apple juice comprising nano-immunomagnetic separation in tandem with surface enhanced Raman scattering. Int J Food Microbiol. 2014;189:89-97.
Najafi, R., Mukherjee, S., Hudson, J., Sharma, A., & Banerjee, P. (2014). Development of a rapid capture-cum-detection method for Escherichia coli O157 from apple juice comprising nano-immunomagnetic separation in tandem with surface enhanced Raman scattering. International Journal of Food Microbiology, 189, 89-97. https://doi.org/10.1016/j.ijfoodmicro.2014.07.036
Najafi R, et al. Development of a Rapid Capture-cum-detection Method for Escherichia Coli O157 From Apple Juice Comprising Nano-immunomagnetic Separation in Tandem With Surface Enhanced Raman Scattering. Int J Food Microbiol. 2014 Oct 17;189:89-97. PubMed PMID: 25133877.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a rapid capture-cum-detection method for Escherichia coli O157 from apple juice comprising nano-immunomagnetic separation in tandem with surface enhanced Raman scattering. AU - Najafi,Roya, AU - Mukherjee,Shubhasish, AU - Hudson,Jim,Jr AU - Sharma,Anup, AU - Banerjee,Pratik, Y1 - 2014/08/07/ PY - 2014/03/18/received PY - 2014/07/25/revised PY - 2014/07/30/accepted PY - 2014/8/19/entrez PY - 2014/8/19/pubmed PY - 2014/11/7/medline KW - Apple juice KW - Escherichia coli O157 KW - Immunomagnetic separation (IMS) KW - Nanoparticles KW - Rapid detection KW - Surface enhanced Raman spectroscopy (SERS) SP - 89 EP - 97 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 189 N2 - A combined capture and detection method comprising of nano-immunomagnetic separation (NIMS) and surface enhanced Raman spectroscopy (SERS) was developed to detect Escherichia coli O157 from liquid media including apple juice. The capture antibodies (cAbs) were immobilized on magnetite-gold (Fe3O4/Au) magnetic nanoparticles (MNPs) which were used for separation and concentration of the E. coli O157 cells from model liquid food matrix. The capture efficiency (CE) for E. coli O157 using MNP was found to be approximately 84-94%. No cross reactivity was observed with background non-target organisms. There was a significant difference in the mean CE of bacteria captured by MNP and commercially sourced immunomagnetic microbeads (p<0.05). For the detection of target pathogen, SERS labels were prepared by conjugating gold nanoparticles with Raman reporter molecules and the detector antibody (dAb). Au-Raman label-dAb was interacted with gold coated MNP-cAb-E. coli O157 complex. The ability of this immunoassay to detect E. coli O157 in apple juice was investigated. We have successfully applied the synthesized Fe3O4/Au nanoclusters to E. coli O157 detection in apple juice using the SERS method. The lowest detectable bacterial cell concentration in apple juice was 10(2)CFU/mL with a total analysis time of less than an hour. This method presents a convenient way of preconcentration, separation, and detection of low levels of target pathogen from liquid food matrix. SN - 1879-3460 UR - https://www.unboundmedicine.com/medline/citation/25133877/Development_of_a_rapid_capture_cum_detection_method_for_Escherichia_coli_O157_from_apple_juice_comprising_nano_immunomagnetic_separation_in_tandem_with_surface_enhanced_Raman_scattering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(14)00391-2 DB - PRIME DP - Unbound Medicine ER -