Tags

Type your tag names separated by a space and hit enter

Lithospermum erythrorhizon extract protects keratinocytes and fibroblasts against oxidative stress.
J Med Food. 2014 Nov; 17(11):1189-96.JM

Abstract

Oxidative stress damages dermal and epidermal cells and degrades extracellular matrix proteins, such as collagen, ultimately leading to skin aging. The present study evaluated the potential protective effect of the aqueous methanolic extract obtained from Lithospermum erythrorhizon (LE) against oxidative stress, induced by H2O2 and ultraviolet (UV) irradiation, on human keratinocyte (HaCaT) and human dermal fibroblast-neonatal (HDF-n) cells. Exposure of cells to H2O2 or UVB irradiation markedly increased oxidative stress and reduced cell viability. However, pretreatment of cells with the LE extract not only increased cell viability (up to 84.5%), but also significantly decreased oxidative stress. Further, the LE extract downregulated the expression of matrix metalloproteinase-1, an endopeptidase that degrades extracellular matrix collagen. In contrast, treatment with the LE extract did not affect the expression of procollagen type 1 in HDF-n cells exposed to UVA irradiation. Thirteen phenolic compounds, including derivatives of shikonin and caffeic acid, were identified by ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. These results suggest that LE-derived extracts may protect oxidative-stress-induced skin aging by inhibiting degradation of skin collagen, and that this protection may derive at least in part from the antioxidant phenolics present in these extracts. Further studies are warranted to determine the potential utility of LE-derived extracts in both therapeutic and cosmetic applications.

Authors+Show Affiliations

1 Department of Food Science and Biotechnology, Kyung Hee University , Yongin, Gyeonggi, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25136892

Citation

Yoo, Hee Geun, et al. "Lithospermum Erythrorhizon Extract Protects Keratinocytes and Fibroblasts Against Oxidative Stress." Journal of Medicinal Food, vol. 17, no. 11, 2014, pp. 1189-96.
Yoo HG, Lee BH, Kim W, et al. Lithospermum erythrorhizon extract protects keratinocytes and fibroblasts against oxidative stress. J Med Food. 2014;17(11):1189-96.
Yoo, H. G., Lee, B. H., Kim, W., Lee, J. S., Kim, G. H., Chun, O. K., Koo, S. I., & Kim, D. O. (2014). Lithospermum erythrorhizon extract protects keratinocytes and fibroblasts against oxidative stress. Journal of Medicinal Food, 17(11), 1189-96. https://doi.org/10.1089/jmf.2013.3088
Yoo HG, et al. Lithospermum Erythrorhizon Extract Protects Keratinocytes and Fibroblasts Against Oxidative Stress. J Med Food. 2014;17(11):1189-96. PubMed PMID: 25136892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lithospermum erythrorhizon extract protects keratinocytes and fibroblasts against oxidative stress. AU - Yoo,Hee Geun, AU - Lee,Bong Han, AU - Kim,Wooki, AU - Lee,Jong Suk, AU - Kim,Gun Hee, AU - Chun,Ock K, AU - Koo,Sung I, AU - Kim,Dae-Ok, Y1 - 2014/08/19/ PY - 2014/8/20/entrez PY - 2014/8/20/pubmed PY - 2015/6/26/medline KW - caffeic acid derivative KW - matrix metalloproteinase-1 KW - shikonin derivative KW - skin KW - ultraviolet irradiation KW - vitamin C equivalent antioxidant capacity SP - 1189 EP - 96 JF - Journal of medicinal food JO - J Med Food VL - 17 IS - 11 N2 - Oxidative stress damages dermal and epidermal cells and degrades extracellular matrix proteins, such as collagen, ultimately leading to skin aging. The present study evaluated the potential protective effect of the aqueous methanolic extract obtained from Lithospermum erythrorhizon (LE) against oxidative stress, induced by H2O2 and ultraviolet (UV) irradiation, on human keratinocyte (HaCaT) and human dermal fibroblast-neonatal (HDF-n) cells. Exposure of cells to H2O2 or UVB irradiation markedly increased oxidative stress and reduced cell viability. However, pretreatment of cells with the LE extract not only increased cell viability (up to 84.5%), but also significantly decreased oxidative stress. Further, the LE extract downregulated the expression of matrix metalloproteinase-1, an endopeptidase that degrades extracellular matrix collagen. In contrast, treatment with the LE extract did not affect the expression of procollagen type 1 in HDF-n cells exposed to UVA irradiation. Thirteen phenolic compounds, including derivatives of shikonin and caffeic acid, were identified by ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. These results suggest that LE-derived extracts may protect oxidative-stress-induced skin aging by inhibiting degradation of skin collagen, and that this protection may derive at least in part from the antioxidant phenolics present in these extracts. Further studies are warranted to determine the potential utility of LE-derived extracts in both therapeutic and cosmetic applications. SN - 1557-7600 UR - https://www.unboundmedicine.com/medline/citation/25136892/Lithospermum_erythrorhizon_extract_protects_keratinocytes_and_fibroblasts_against_oxidative_stress_ L2 - https://www.liebertpub.com/doi/full/10.1089/jmf.2013.3088?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -