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Nuclear factor I-C (NFIC) regulates dentin sialophosphoprotein (DSPP) and E-cadherin via control of Krüppel-like factor 4 (KLF4) during dentinogenesis.
J Biol Chem. 2014 Oct 10; 289(41):28225-36.JB

Abstract

Odontoblasts are a type of terminally differentiated matrix-secreting cells. A number of molecular mechanisms are involved in the differentiation of odontoblasts. Several studies demonstrated that Krüppel-like factor 4 (KLF4) promotes odontoblast differentiation via control of dentin sialophosphoprotein (DSPP). Because nuclear factor I-C (NFIC) is also known to control DSPP, we investigated the relationship between NFIC and KLF4 during odontoblast differentiation. Klf4 mRNA expression was significantly decreased in Nfic(-/-) pulp cells compared with wild type cells. In immunohistochemistry assays, dentin matrix protein 1 (Dmp1), and DSP protein expression was barely observed in Nfic(-/-) odontoblasts and dentin matrix. Nfic bound directly to the Klf4 promoter and stimulated Klf4 transcriptional activity, thereby regulating Dmp1 and DSPP expression during odontoblast differentiation. Nfic or Klf4 overexpression promoted mineralized nodule formation in MDPC-23 cells. In addition, Nfic overexpression also decreased Slug luciferase activity but augmented E-cadherin promoter activity via up-regulation of Klf4 in odontoblasts. Our study reveals important signaling pathways during dentinogenesis: the Nfic-Klf4-Dmp1-Dspp and the Nfic-Klf4-E-cadherin pathways in odontoblasts. Our results indicate the important role of NFIC in regulating KLF4 during dentinogenesis.

Authors+Show Affiliations

From the Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul 110-749, Korea and.From the Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul 110-749, Korea and.From the Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul 110-749, Korea and.the Department of Dental Hygiene, Namseoul University, Cheon-An, Chung-Nam 331-707, Korea.the Department of Dental Hygiene, Namseoul University, Cheon-An, Chung-Nam 331-707, Korea.From the Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul 110-749, Korea and jcapark@snu.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25138274

Citation

Lee, Hye-Kyung, et al. "Nuclear Factor I-C (NFIC) Regulates Dentin Sialophosphoprotein (DSPP) and E-cadherin Via Control of Krüppel-like Factor 4 (KLF4) During Dentinogenesis." The Journal of Biological Chemistry, vol. 289, no. 41, 2014, pp. 28225-36.
Lee HK, Lee DS, Park SJ, et al. Nuclear factor I-C (NFIC) regulates dentin sialophosphoprotein (DSPP) and E-cadherin via control of Krüppel-like factor 4 (KLF4) during dentinogenesis. J Biol Chem. 2014;289(41):28225-36.
Lee, H. K., Lee, D. S., Park, S. J., Cho, K. H., Bae, H. S., & Park, J. C. (2014). Nuclear factor I-C (NFIC) regulates dentin sialophosphoprotein (DSPP) and E-cadherin via control of Krüppel-like factor 4 (KLF4) during dentinogenesis. The Journal of Biological Chemistry, 289(41), 28225-36. https://doi.org/10.1074/jbc.M114.568691
Lee HK, et al. Nuclear Factor I-C (NFIC) Regulates Dentin Sialophosphoprotein (DSPP) and E-cadherin Via Control of Krüppel-like Factor 4 (KLF4) During Dentinogenesis. J Biol Chem. 2014 Oct 10;289(41):28225-36. PubMed PMID: 25138274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nuclear factor I-C (NFIC) regulates dentin sialophosphoprotein (DSPP) and E-cadherin via control of Krüppel-like factor 4 (KLF4) during dentinogenesis. AU - Lee,Hye-Kyung, AU - Lee,Dong-Seol, AU - Park,Su-Jin, AU - Cho,Kwang-Hee, AU - Bae,Hyun-Sook, AU - Park,Joo-Cheol, Y1 - 2014/08/19/ PY - 2014/8/21/entrez PY - 2014/8/21/pubmed PY - 2015/2/7/medline KW - DSPP KW - Dentin KW - Dentinogenesis KW - Differentiation KW - Gene Regulation KW - KLF5 KW - NFIC KW - Odontoblast KW - Signaling KW - Tooth Development SP - 28225 EP - 36 JF - The Journal of biological chemistry JO - J Biol Chem VL - 289 IS - 41 N2 - Odontoblasts are a type of terminally differentiated matrix-secreting cells. A number of molecular mechanisms are involved in the differentiation of odontoblasts. Several studies demonstrated that Krüppel-like factor 4 (KLF4) promotes odontoblast differentiation via control of dentin sialophosphoprotein (DSPP). Because nuclear factor I-C (NFIC) is also known to control DSPP, we investigated the relationship between NFIC and KLF4 during odontoblast differentiation. Klf4 mRNA expression was significantly decreased in Nfic(-/-) pulp cells compared with wild type cells. In immunohistochemistry assays, dentin matrix protein 1 (Dmp1), and DSP protein expression was barely observed in Nfic(-/-) odontoblasts and dentin matrix. Nfic bound directly to the Klf4 promoter and stimulated Klf4 transcriptional activity, thereby regulating Dmp1 and DSPP expression during odontoblast differentiation. Nfic or Klf4 overexpression promoted mineralized nodule formation in MDPC-23 cells. In addition, Nfic overexpression also decreased Slug luciferase activity but augmented E-cadherin promoter activity via up-regulation of Klf4 in odontoblasts. Our study reveals important signaling pathways during dentinogenesis: the Nfic-Klf4-Dmp1-Dspp and the Nfic-Klf4-E-cadherin pathways in odontoblasts. Our results indicate the important role of NFIC in regulating KLF4 during dentinogenesis. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/25138274/Nuclear_factor_I_C__NFIC__regulates_dentin_sialophosphoprotein__DSPP__and_E_cadherin_via_control_of_Krüppel_like_factor_4__KLF4__during_dentinogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(20)37083-6 DB - PRIME DP - Unbound Medicine ER -