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B chromosomes in the grasshopper Eyprepocnemis plorans are present in all body parts analyzed and show extensive variation for rDNA copy number.
Cytogenet Genome Res. 2014; 143(4):268-74.CG

Abstract

B chromosomes in the grasshopper Eyprepocnemis plorans are considered to be mitotically stable, because all meiotic (primary spermatocytes and oocytes) or mitotic (embryos, ovarioles, and gastric caecum) cells analyzed within the same individual show the same B chromosome number. Nothing is known, however, about body parts with somatic tissues with no mitotic activity in adult individuals, constituting the immense majority of their body. Therefore, we investigated whether B chromosomes are present in 8 non-mitotically active somatic body parts from both sexes in addition to ovarioles and testes by PCR analysis of 2 B-specific molecular markers. We also elucidated the number of B chromosomes that an individual carried through quantifying the B-located rDNA copy number by qPCR. Our results indicated the amplification of both B-specific markers in all analyzed body parts. However, we found high variation between males for the estimated number of rDNA units in the B chromosomes. These results demonstrate the presence of B chromosomes in all body parts from the same individual and suggest a high variation in the rDNA content of the B chromosomes carried by different individuals from the same population, presumably due to unequal crossovers during meiosis.

Authors+Show Affiliations

Departamento de Genética, Facultad de Ciencias, Universidad de Granada, Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25138924

Citation

Ruiz-Estévez, Mercedes, et al. "B Chromosomes in the Grasshopper Eyprepocnemis Plorans Are Present in All Body Parts Analyzed and Show Extensive Variation for rDNA Copy Number." Cytogenetic and Genome Research, vol. 143, no. 4, 2014, pp. 268-74.
Ruiz-Estévez M, Cabrero J, Camacho JP, et al. B chromosomes in the grasshopper Eyprepocnemis plorans are present in all body parts analyzed and show extensive variation for rDNA copy number. Cytogenet Genome Res. 2014;143(4):268-74.
Ruiz-Estévez, M., Cabrero, J., Camacho, J. P., & López-León, M. D. (2014). B chromosomes in the grasshopper Eyprepocnemis plorans are present in all body parts analyzed and show extensive variation for rDNA copy number. Cytogenetic and Genome Research, 143(4), 268-74. https://doi.org/10.1159/000365797
Ruiz-Estévez M, et al. B Chromosomes in the Grasshopper Eyprepocnemis Plorans Are Present in All Body Parts Analyzed and Show Extensive Variation for rDNA Copy Number. Cytogenet Genome Res. 2014;143(4):268-74. PubMed PMID: 25138924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - B chromosomes in the grasshopper Eyprepocnemis plorans are present in all body parts analyzed and show extensive variation for rDNA copy number. AU - Ruiz-Estévez,Mercedes, AU - Cabrero,Josefa, AU - Camacho,Juan Pedro M, AU - López-León,María Dolores, Y1 - 2014/08/16/ PY - 2014/03/26/accepted PY - 2014/8/21/entrez PY - 2014/8/21/pubmed PY - 2014/12/20/medline SP - 268 EP - 74 JF - Cytogenetic and genome research JO - Cytogenet Genome Res VL - 143 IS - 4 N2 - B chromosomes in the grasshopper Eyprepocnemis plorans are considered to be mitotically stable, because all meiotic (primary spermatocytes and oocytes) or mitotic (embryos, ovarioles, and gastric caecum) cells analyzed within the same individual show the same B chromosome number. Nothing is known, however, about body parts with somatic tissues with no mitotic activity in adult individuals, constituting the immense majority of their body. Therefore, we investigated whether B chromosomes are present in 8 non-mitotically active somatic body parts from both sexes in addition to ovarioles and testes by PCR analysis of 2 B-specific molecular markers. We also elucidated the number of B chromosomes that an individual carried through quantifying the B-located rDNA copy number by qPCR. Our results indicated the amplification of both B-specific markers in all analyzed body parts. However, we found high variation between males for the estimated number of rDNA units in the B chromosomes. These results demonstrate the presence of B chromosomes in all body parts from the same individual and suggest a high variation in the rDNA content of the B chromosomes carried by different individuals from the same population, presumably due to unequal crossovers during meiosis. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/25138924/B_chromosomes_in_the_grasshopper_Eyprepocnemis_plorans_are_present_in_all_body_parts_analyzed_and_show_extensive_variation_for_rDNA_copy_number_ L2 - https://www.karger.com?DOI=10.1159/000365797 DB - PRIME DP - Unbound Medicine ER -