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Vortex-assisted surfactant-enhanced-emulsification liquid-liquid microextraction of biogenic amines in fermented foods before their simultaneous analysis by high-performance liquid chromatography.
J Sep Sci. 2014 Nov; 37(21):3164-73.JS

Abstract

A simple, rapid, sensitive, and environmentally friendly method, based on modified dispersive liquid-liquid microextraction coupled with high-performance liquid chromatography was developed for the simultaneous determination of five biogenic amines in fermented food samples. Biogenic amines were derivatized with 9-fluorenylmethyl chloroformate, extracted by vortex-assisted surfactant-enhanced emulsification liquid-liquid microextraction, and then analyzed by high-performance liquid chromatography. Five biogenic amine compounds were separated within 30 min using a C18 column and gradient elution with acetonitrile and 1% acetic acid. Factors influencing the derivatization and extraction efficiency such as type and volume of extraction solvent, type, and concentration of surfactant, pH, salt addition, and vortex time were optimized. Under the optimum conditions, the method provided the enrichment factors in the range of 161-553. Good linearity was obtained from 0.002-0.5 mg/L for cadaverine and tyramine, 0.003-1 mg/L for tryptamine and histamine, and 0.005-1 mg/L for spermidine with coefficient of determination (R(2)) > 0.992. The limits of detection ranged from 0.0010 to 0.0026 mg/L. The proposed method was successfully applied to analysis of biogenic amines in fermented foods such as fermented fish (plaa-som), wine and beer where good recoveries were obtained in the range of 83.2-112.5%

Authors+Show Affiliations

Department of Chemistry and Center of Excellence for Innovation in Chemistry, Materials Chemistry Research Center, Khon Kaen University, Khon Kaen, Thailand.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25142110

Citation

Donthuan, Jaruwan, et al. "Vortex-assisted Surfactant-enhanced-emulsification Liquid-liquid Microextraction of Biogenic Amines in Fermented Foods Before Their Simultaneous Analysis By High-performance Liquid Chromatography." Journal of Separation Science, vol. 37, no. 21, 2014, pp. 3164-73.
Donthuan J, Yunchalard S, Srijaranai S. Vortex-assisted surfactant-enhanced-emulsification liquid-liquid microextraction of biogenic amines in fermented foods before their simultaneous analysis by high-performance liquid chromatography. J Sep Sci. 2014;37(21):3164-73.
Donthuan, J., Yunchalard, S., & Srijaranai, S. (2014). Vortex-assisted surfactant-enhanced-emulsification liquid-liquid microextraction of biogenic amines in fermented foods before their simultaneous analysis by high-performance liquid chromatography. Journal of Separation Science, 37(21), 3164-73. https://doi.org/10.1002/jssc.201400570
Donthuan J, Yunchalard S, Srijaranai S. Vortex-assisted Surfactant-enhanced-emulsification Liquid-liquid Microextraction of Biogenic Amines in Fermented Foods Before Their Simultaneous Analysis By High-performance Liquid Chromatography. J Sep Sci. 2014;37(21):3164-73. PubMed PMID: 25142110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vortex-assisted surfactant-enhanced-emulsification liquid-liquid microextraction of biogenic amines in fermented foods before their simultaneous analysis by high-performance liquid chromatography. AU - Donthuan,Jaruwan, AU - Yunchalard,Sirinda, AU - Srijaranai,Supalax, Y1 - 2014/09/23/ PY - 2014/05/27/received PY - 2014/08/04/revised PY - 2014/08/05/accepted PY - 2014/8/22/entrez PY - 2014/8/22/pubmed PY - 2015/6/24/medline KW - 9-Fluorenylmethyl chloroformate KW - Biogenic amines KW - Fermented foods KW - liquid-liquid microextraction SP - 3164 EP - 73 JF - Journal of separation science JO - J Sep Sci VL - 37 IS - 21 N2 - A simple, rapid, sensitive, and environmentally friendly method, based on modified dispersive liquid-liquid microextraction coupled with high-performance liquid chromatography was developed for the simultaneous determination of five biogenic amines in fermented food samples. Biogenic amines were derivatized with 9-fluorenylmethyl chloroformate, extracted by vortex-assisted surfactant-enhanced emulsification liquid-liquid microextraction, and then analyzed by high-performance liquid chromatography. Five biogenic amine compounds were separated within 30 min using a C18 column and gradient elution with acetonitrile and 1% acetic acid. Factors influencing the derivatization and extraction efficiency such as type and volume of extraction solvent, type, and concentration of surfactant, pH, salt addition, and vortex time were optimized. Under the optimum conditions, the method provided the enrichment factors in the range of 161-553. Good linearity was obtained from 0.002-0.5 mg/L for cadaverine and tyramine, 0.003-1 mg/L for tryptamine and histamine, and 0.005-1 mg/L for spermidine with coefficient of determination (R(2)) > 0.992. The limits of detection ranged from 0.0010 to 0.0026 mg/L. The proposed method was successfully applied to analysis of biogenic amines in fermented foods such as fermented fish (plaa-som), wine and beer where good recoveries were obtained in the range of 83.2-112.5% SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/25142110/Vortex_assisted_surfactant_enhanced_emulsification_liquid_liquid_microextraction_of_biogenic_amines_in_fermented_foods_before_their_simultaneous_analysis_by_high_performance_liquid_chromatography_ L2 - https://doi.org/10.1002/jssc.201400570 DB - PRIME DP - Unbound Medicine ER -