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Proteomic analyses of ethanol tolerance in Lactobacillus buchneri NRRL B-30929.
Proteomics 2014; 14(21-22):2540-4P

Abstract

The Lactobacillus buchneri NRRL B-30929 strain, isolated from a fuel ethanol (EtOH) production facility, exhibits high tolerance to environmental EtOH concentrations. This study aimed to identify proteins produced by B-30929 in response to environmental EtOH. Cellular proteins expressed by B-30929 growing in media with 10 versus 0% EtOH were compared by 2DE, followed by in-gel digestion and MALDI-MS analyses. Twenty EtOH responsive proteins were identified. These include a proline-specific peptidase (Lbuc_1852); a membrane protein (Lbuc_0921), two general stress-related proteins including a 10 kDa chaperonin (GroESL Lbuc_1359) and a 29 kDa member of the HK 97 family (Lbuc_1523); metabolic enzymes involving redox potential balances (Lbuc_2051 and Lbuc_0522) and carbohydrate fermentation (Lbuc_1319 and Lbuc_2157); nitrogen, amino acid, and fatty acid metabolism proteins (Lbuc_1994, Lbuc_0446, Lbuc_0858, Lbuc_0707, and Lbuc_0787). These changes suggested B-30929 cells respond to EtOH by degradation of available proteins and fatty acids and increased production of specific enzymes and molecular chaperons. These results can be used to guide genetic modifications to increase EtOH tolerance in industrial biocatalysts. The data have been deposited to World-2DPAGE (http://world-2dpage.expasy.org/repository/0068/; username liu, password 1h8d6Mg1).

Authors+Show Affiliations

Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25143305

Citation

Liu, Siqing. "Proteomic Analyses of Ethanol Tolerance in Lactobacillus Buchneri NRRL B-30929." Proteomics, vol. 14, no. 21-22, 2014, pp. 2540-4.
Liu S. Proteomic analyses of ethanol tolerance in Lactobacillus buchneri NRRL B-30929. Proteomics. 2014;14(21-22):2540-4.
Liu, S. (2014). Proteomic analyses of ethanol tolerance in Lactobacillus buchneri NRRL B-30929. Proteomics, 14(21-22), pp. 2540-4. doi:10.1002/pmic.201300538.
Liu S. Proteomic Analyses of Ethanol Tolerance in Lactobacillus Buchneri NRRL B-30929. Proteomics. 2014;14(21-22):2540-4. PubMed PMID: 25143305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic analyses of ethanol tolerance in Lactobacillus buchneri NRRL B-30929. A1 - Liu,Siqing, Y1 - 2014/09/22/ PY - 2013/12/12/received PY - 2014/06/20/revised PY - 2014/08/15/accepted PY - 2014/8/22/entrez PY - 2014/8/22/pubmed PY - 2015/10/31/medline KW - 2DE KW - Ethanol tolerance KW - Lactobacillus buchneri KW - Microbiology SP - 2540 EP - 4 JF - Proteomics JO - Proteomics VL - 14 IS - 21-22 N2 - The Lactobacillus buchneri NRRL B-30929 strain, isolated from a fuel ethanol (EtOH) production facility, exhibits high tolerance to environmental EtOH concentrations. This study aimed to identify proteins produced by B-30929 in response to environmental EtOH. Cellular proteins expressed by B-30929 growing in media with 10 versus 0% EtOH were compared by 2DE, followed by in-gel digestion and MALDI-MS analyses. Twenty EtOH responsive proteins were identified. These include a proline-specific peptidase (Lbuc_1852); a membrane protein (Lbuc_0921), two general stress-related proteins including a 10 kDa chaperonin (GroESL Lbuc_1359) and a 29 kDa member of the HK 97 family (Lbuc_1523); metabolic enzymes involving redox potential balances (Lbuc_2051 and Lbuc_0522) and carbohydrate fermentation (Lbuc_1319 and Lbuc_2157); nitrogen, amino acid, and fatty acid metabolism proteins (Lbuc_1994, Lbuc_0446, Lbuc_0858, Lbuc_0707, and Lbuc_0787). These changes suggested B-30929 cells respond to EtOH by degradation of available proteins and fatty acids and increased production of specific enzymes and molecular chaperons. These results can be used to guide genetic modifications to increase EtOH tolerance in industrial biocatalysts. The data have been deposited to World-2DPAGE (http://world-2dpage.expasy.org/repository/0068/; username liu, password 1h8d6Mg1). SN - 1615-9861 UR - https://www.unboundmedicine.com/medline/citation/25143305/Proteomic_analyses_of_ethanol_tolerance_in_Lactobacillus_buchneri_NRRL_B_30929_ L2 - https://doi.org/10.1002/pmic.201300538 DB - PRIME DP - Unbound Medicine ER -