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[Laboratory methods in the diagnosis of coccidioidomycosis].
Rev Chilena Infectol. 2014 Jun; 31(3):293-7.RC

Abstract

Coccidioides immitis and Coccidioides posadasii, the two recognized causes of coccidioidomicosis, may be diagnosed by direct microscopy, culture, serologic and the skin-test with mycelial coccidioidin. Identification of spherules by direct examination of secretions after addition of KOH, is more rapid and may speed diagnosis. Histopathology of infected tissue demonstrates acute and chronic granulomatous inflammation and in the section strained with hematoxylin-eosine (HE) or Grocott metamine-silver (GMS). Isolation of Coccidioides spp. by culture is not difficult, the fungus grows well on most mycologic media after 5-7 days of incubation and the laboratory should maintain a biological safety level 2 or 3. Phenotypic identification to genus level may be achieved by visualization of arthroconidia in culture. Isolates may be confirmed as Coccidioides ssp. by molecular probes, but separation of species is best achieved by specialized molecular techniques, available at the Reference Laboratory. Immune diffusion and enzyme-immune assays (EIA) are commonly used for detection of IgM and IgG antibodies in serum. Sequential complement fixations (CF) for IgG class of antibody are useful for the prognosis of coccidioidomycosis.

Authors

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Pub Type(s)

Journal Article

Language

spa

PubMed ID

25146203

Citation

Carrada-Bravo, Teodoro. "[Laboratory Methods in the Diagnosis of Coccidioidomycosis]." Revista Chilena De Infectologia : Organo Oficial De La Sociedad Chilena De Infectologia, vol. 31, no. 3, 2014, pp. 293-7.
Carrada-Bravo T. [Laboratory methods in the diagnosis of coccidioidomycosis]. Rev Chilena Infectol. 2014;31(3):293-7.
Carrada-Bravo, T. (2014). [Laboratory methods in the diagnosis of coccidioidomycosis]. Revista Chilena De Infectologia : Organo Oficial De La Sociedad Chilena De Infectologia, 31(3), 293-7. https://doi.org/10.4067/S0716-10182014000300007
Carrada-Bravo T. [Laboratory Methods in the Diagnosis of Coccidioidomycosis]. Rev Chilena Infectol. 2014;31(3):293-7. PubMed PMID: 25146203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Laboratory methods in the diagnosis of coccidioidomycosis]. A1 - Carrada-Bravo,Teodoro, PY - 2013/05/26/received PY - 2014/04/07/accepted PY - 2014/8/23/entrez PY - 2014/8/26/pubmed PY - 2015/8/5/medline SP - 293 EP - 7 JF - Revista chilena de infectologia : organo oficial de la Sociedad Chilena de Infectologia JO - Rev Chilena Infectol VL - 31 IS - 3 N2 - Coccidioides immitis and Coccidioides posadasii, the two recognized causes of coccidioidomicosis, may be diagnosed by direct microscopy, culture, serologic and the skin-test with mycelial coccidioidin. Identification of spherules by direct examination of secretions after addition of KOH, is more rapid and may speed diagnosis. Histopathology of infected tissue demonstrates acute and chronic granulomatous inflammation and in the section strained with hematoxylin-eosine (HE) or Grocott metamine-silver (GMS). Isolation of Coccidioides spp. by culture is not difficult, the fungus grows well on most mycologic media after 5-7 days of incubation and the laboratory should maintain a biological safety level 2 or 3. Phenotypic identification to genus level may be achieved by visualization of arthroconidia in culture. Isolates may be confirmed as Coccidioides ssp. by molecular probes, but separation of species is best achieved by specialized molecular techniques, available at the Reference Laboratory. Immune diffusion and enzyme-immune assays (EIA) are commonly used for detection of IgM and IgG antibodies in serum. Sequential complement fixations (CF) for IgG class of antibody are useful for the prognosis of coccidioidomycosis. SN - 0716-1018 UR - https://www.unboundmedicine.com/medline/citation/25146203/[Laboratory_methods_in_the_diagnosis_of_coccidioidomycosis]_ L2 - http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182014000300007&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -