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N-Acetylcysteine Ameliorates Experimental Autoimmune Myocarditis in Rats via Nitric Oxide.
J Cardiovasc Pharmacol Ther. 2015 Mar; 20(2):203-10.JC

Abstract

BACKGROUND

Oxidative stress may play an important role in the development of myocarditis. We investigated the effects of N-acetylcysteine (NAC), a potent antioxidant, on experimental autoimmune myocarditis (EAM) in rats.

METHODS AND RESULTS

A rat model of porcine myosin-induced EAM was used. After the immunization with myosin, NAC (20 mg/kg/d) or saline was injected intraperitoneally on days 1 to 21. Additional myosin-immunized rats treated with NAC were orally given 25 mg/kg/d of N(G)-nitro-l-arginine methylester (l-NAME), an inhibitor of nitric oxide (NO) synthase, and N(G)-nitro-d-arginine methylester (d-NAME), an inactive enantiomer. The NAC treatment improved cardiac pathology associated with reduced superoxide production. In the EAM rats treated with NAC associated with oral l-NAME, but not with oral d-NAME, the severity of myocarditis was not reduced. Expression of intercellular adhesion molecule 1 was reduced by NAC treatment. Myocardial c-kit(+) cells were demonstrated only in the NAC-treated group. Hemodynamic study showed that the increased left ventricular mass produced by myocardial inflammation tended to be reduced by NAC treatment.

CONCLUSION

Treatment with NAC ameliorated myocardial injury via NO system in a rat model of myocarditis.

Authors+Show Affiliations

Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.First Department of Internal Medicine, Faculty of Medical Science, University of Fukui, Fukui, Japan.First Department of Internal Medicine, Faculty of Medical Science, University of Fukui, Fukui, Japan.First Department of Internal Medicine, Faculty of Medical Science, University of Fukui, Fukui, Japan.Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan kkishi@kuhp.kyoto-u.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25147347

Citation

Shimada, Kana, et al. "N-Acetylcysteine Ameliorates Experimental Autoimmune Myocarditis in Rats Via Nitric Oxide." Journal of Cardiovascular Pharmacology and Therapeutics, vol. 20, no. 2, 2015, pp. 203-10.
Shimada K, Uzui H, Ueda T, et al. N-Acetylcysteine Ameliorates Experimental Autoimmune Myocarditis in Rats via Nitric Oxide. J Cardiovasc Pharmacol Ther. 2015;20(2):203-10.
Shimada, K., Uzui, H., Ueda, T., Lee, J. D., & Kishimoto, C. (2015). N-Acetylcysteine Ameliorates Experimental Autoimmune Myocarditis in Rats via Nitric Oxide. Journal of Cardiovascular Pharmacology and Therapeutics, 20(2), 203-10. https://doi.org/10.1177/1074248414547574
Shimada K, et al. N-Acetylcysteine Ameliorates Experimental Autoimmune Myocarditis in Rats Via Nitric Oxide. J Cardiovasc Pharmacol Ther. 2015;20(2):203-10. PubMed PMID: 25147347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-Acetylcysteine Ameliorates Experimental Autoimmune Myocarditis in Rats via Nitric Oxide. AU - Shimada,Kana, AU - Uzui,Hiroyasu, AU - Ueda,Takanori, AU - Lee,Jong-Dae, AU - Kishimoto,Chiharu, Y1 - 2014/08/20/ PY - 2014/8/23/entrez PY - 2014/8/26/pubmed PY - 2016/2/9/medline KW - N-acetylcysteine KW - autoimmune myocarditis KW - free radicals KW - nitric oxide KW - oxidative stress SP - 203 EP - 10 JF - Journal of cardiovascular pharmacology and therapeutics JO - J. Cardiovasc. Pharmacol. Ther. VL - 20 IS - 2 N2 - BACKGROUND: Oxidative stress may play an important role in the development of myocarditis. We investigated the effects of N-acetylcysteine (NAC), a potent antioxidant, on experimental autoimmune myocarditis (EAM) in rats. METHODS AND RESULTS: A rat model of porcine myosin-induced EAM was used. After the immunization with myosin, NAC (20 mg/kg/d) or saline was injected intraperitoneally on days 1 to 21. Additional myosin-immunized rats treated with NAC were orally given 25 mg/kg/d of N(G)-nitro-l-arginine methylester (l-NAME), an inhibitor of nitric oxide (NO) synthase, and N(G)-nitro-d-arginine methylester (d-NAME), an inactive enantiomer. The NAC treatment improved cardiac pathology associated with reduced superoxide production. In the EAM rats treated with NAC associated with oral l-NAME, but not with oral d-NAME, the severity of myocarditis was not reduced. Expression of intercellular adhesion molecule 1 was reduced by NAC treatment. Myocardial c-kit(+) cells were demonstrated only in the NAC-treated group. Hemodynamic study showed that the increased left ventricular mass produced by myocardial inflammation tended to be reduced by NAC treatment. CONCLUSION: Treatment with NAC ameliorated myocardial injury via NO system in a rat model of myocarditis. SN - 1940-4034 UR - https://www.unboundmedicine.com/medline/citation/25147347/N_Acetylcysteine_Ameliorates_Experimental_Autoimmune_Myocarditis_in_Rats_via_Nitric_Oxide_ L2 - http://journals.sagepub.com/doi/full/10.1177/1074248414547574?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -