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Optimization of reflux conditions for total flavonoid and total phenolic extraction and enhanced antioxidant capacity in Pandan (Pandanus amaryllifolius Roxb.) using response surface methodology.
ScientificWorldJournal. 2014; 2014:523120.S

Abstract

Response surface methodology was applied to optimization of the conditions for reflux extraction of Pandan (Pandanus amaryllifolius Roxb.) in order to achieve a high content of total flavonoids (TF), total phenolics (TP), and high antioxidant capacity (AC) in the extracts. Central composite experimental design with three factors and three levels was employed to consider the effects of the operation parameters, including the methanol concentration (MC, 40%-80%), extraction temperature (ET, 40-70°C), and liquid-to-solid ratio (LS ratio, 20-40 mL/g) on the properties of the extracts. Response surface plots showed that increasing these operation parameters induced the responses significantly. The TF content and AC could be maximized when the extraction conditions (MC, ET, and LS ratio) were 78.8%, 69.5°C, and 32.4 mL/g, respectively, whereas the TP content was optimal when these variables were 75.1%, 70°C, and 31.8 mL/g, respectively. Under these optimum conditions, the experimental TF and TP content and AC were 1.78, 6.601 mg/g DW, and 87.38%, respectively. The optimized model was validated by a comparison of the predicted and experimental values. The experimental values were found to be in agreement with the predicted values, indicating the suitability of the model for optimizing the conditions for the reflux extraction of Pandan.

Authors+Show Affiliations

Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25147852

Citation

Ghasemzadeh, Ali, and Hawa Z E. Jaafar. "Optimization of Reflux Conditions for Total Flavonoid and Total Phenolic Extraction and Enhanced Antioxidant Capacity in Pandan (Pandanus Amaryllifolius Roxb.) Using Response Surface Methodology." TheScientificWorldJournal, vol. 2014, 2014, p. 523120.
Ghasemzadeh A, Jaafar HZ. Optimization of reflux conditions for total flavonoid and total phenolic extraction and enhanced antioxidant capacity in Pandan (Pandanus amaryllifolius Roxb.) using response surface methodology. ScientificWorldJournal. 2014;2014:523120.
Ghasemzadeh, A., & Jaafar, H. Z. (2014). Optimization of reflux conditions for total flavonoid and total phenolic extraction and enhanced antioxidant capacity in Pandan (Pandanus amaryllifolius Roxb.) using response surface methodology. TheScientificWorldJournal, 2014, 523120. https://doi.org/10.1155/2014/523120
Ghasemzadeh A, Jaafar HZ. Optimization of Reflux Conditions for Total Flavonoid and Total Phenolic Extraction and Enhanced Antioxidant Capacity in Pandan (Pandanus Amaryllifolius Roxb.) Using Response Surface Methodology. ScientificWorldJournal. 2014;2014:523120. PubMed PMID: 25147852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of reflux conditions for total flavonoid and total phenolic extraction and enhanced antioxidant capacity in Pandan (Pandanus amaryllifolius Roxb.) using response surface methodology. AU - Ghasemzadeh,Ali, AU - Jaafar,Hawa Z E, Y1 - 2014/07/23/ PY - 2014/04/11/received PY - 2014/06/06/revised PY - 2014/07/03/accepted PY - 2014/8/23/entrez PY - 2014/8/26/pubmed PY - 2015/11/3/medline SP - 523120 EP - 523120 JF - TheScientificWorldJournal JO - ScientificWorldJournal VL - 2014 N2 - Response surface methodology was applied to optimization of the conditions for reflux extraction of Pandan (Pandanus amaryllifolius Roxb.) in order to achieve a high content of total flavonoids (TF), total phenolics (TP), and high antioxidant capacity (AC) in the extracts. Central composite experimental design with three factors and three levels was employed to consider the effects of the operation parameters, including the methanol concentration (MC, 40%-80%), extraction temperature (ET, 40-70°C), and liquid-to-solid ratio (LS ratio, 20-40 mL/g) on the properties of the extracts. Response surface plots showed that increasing these operation parameters induced the responses significantly. The TF content and AC could be maximized when the extraction conditions (MC, ET, and LS ratio) were 78.8%, 69.5°C, and 32.4 mL/g, respectively, whereas the TP content was optimal when these variables were 75.1%, 70°C, and 31.8 mL/g, respectively. Under these optimum conditions, the experimental TF and TP content and AC were 1.78, 6.601 mg/g DW, and 87.38%, respectively. The optimized model was validated by a comparison of the predicted and experimental values. The experimental values were found to be in agreement with the predicted values, indicating the suitability of the model for optimizing the conditions for the reflux extraction of Pandan. SN - 1537-744X UR - https://www.unboundmedicine.com/medline/citation/25147852/Optimization_of_reflux_conditions_for_total_flavonoid_and_total_phenolic_extraction_and_enhanced_antioxidant_capacity_in_Pandan__Pandanus_amaryllifolius_Roxb___using_response_surface_methodology_ L2 - https://dx.doi.org/10.1155/2014/523120 DB - PRIME DP - Unbound Medicine ER -