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Selenite enhances immune response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans.
PLoS One. 2014; 9(8):e105810.Plos

Abstract

BACKGROUND

Selenium (Se) is an important nutrient that carries out many biological processes including maintaining optimal immune function. Here, inorganic selenite (Se(IV)) was evaluated for its pathogen resistance and potential-associated factors in Caenorhabditis elegans. The immune effects of Se(IV) were investigated by examining the responses of C. elegans to Pseudomonas aerugonisa PA14 strain.

PRINCIPAL FINDINGS

Se(IV)-treated C. elegans showed increased survival under PA14 infection compared with untreated controls. The significant pathogen resistance of Se(IV) on C. elegans might not be attributed to the effects of Se(IV) on PA14 as Se(IV) showed no effect on bacterial quorum-sensing and virulence factors of PA14. This study showed that Se(IV) enhanced the expression of a gene pivotal for the innate immunity in C. elegans. The study found that the pathogen-resistant phenotypes contributed by Se(IV) was absent from the skn-1 mutant worms. Moreover, Se(IV) influenced the subcellular distribution of SKN-1/Nrf in C. elegans upon PA14 infection. Furthermore, Se(IV) increased mRNA levels of SKN-1 target genes (gst-4 and gcs-1).

CONCLUSIONS

This study found evidence of Se(IV) protecting C. elegans against P. aeruginosa PA14 infection by exerting effects on the innate immunity of C. elegans that is likely mediated via regulation of a SKN-1-dependent signaling pathway.

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Authors+Show Affiliations

Li WH
Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei, Taiwan.
Chang CH
Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei, Taiwan.
Huang CW
Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei, Taiwan.
Wei CC
Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei, Taiwan.
Liao VH
Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei, Taiwan.

MeSH

AnimalsAnimals, Genetically ModifiedBiofilmsCaenorhabditis elegansCaenorhabditis elegans ProteinsDNA-Binding ProteinsGene Expression RegulationGlutamate-Cysteine LigaseGlutathione TransferasePseudomonas aeruginosaQuorum SensingSelenious AcidTranscription FactorsVirulence Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25147937

Citation

Li, Wen-Hsuan, et al. "Selenite Enhances Immune Response Against Pseudomonas Aeruginosa PA14 Via SKN-1 in Caenorhabditis Elegans." PloS One, vol. 9, no. 8, 2014, pp. e105810.
Li WH, Chang CH, Huang CW, et al. Selenite enhances immune response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans. PLoS One. 2014;9(8):e105810.
Li, W. H., Chang, C. H., Huang, C. W., Wei, C. C., & Liao, V. H. (2014). Selenite enhances immune response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans. PloS One, 9(8), e105810. https://doi.org/10.1371/journal.pone.0105810
Li WH, et al. Selenite Enhances Immune Response Against Pseudomonas Aeruginosa PA14 Via SKN-1 in Caenorhabditis Elegans. PLoS One. 2014;9(8):e105810. PubMed PMID: 25147937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenite enhances immune response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans. AU - Li,Wen-Hsuan, AU - Chang,Chun-Han, AU - Huang,Chi-Wei, AU - Wei,Chia-Cheng, AU - Liao,Vivian Hsiu-Chuan, Y1 - 2014/08/22/ PY - 2014/06/16/received PY - 2014/07/23/accepted PY - 2014/8/23/entrez PY - 2014/8/26/pubmed PY - 2016/3/18/medline SP - e105810 EP - e105810 JF - PloS one JO - PLoS One VL - 9 IS - 8 N2 - BACKGROUND: Selenium (Se) is an important nutrient that carries out many biological processes including maintaining optimal immune function. Here, inorganic selenite (Se(IV)) was evaluated for its pathogen resistance and potential-associated factors in Caenorhabditis elegans. The immune effects of Se(IV) were investigated by examining the responses of C. elegans to Pseudomonas aerugonisa PA14 strain. PRINCIPAL FINDINGS: Se(IV)-treated C. elegans showed increased survival under PA14 infection compared with untreated controls. The significant pathogen resistance of Se(IV) on C. elegans might not be attributed to the effects of Se(IV) on PA14 as Se(IV) showed no effect on bacterial quorum-sensing and virulence factors of PA14. This study showed that Se(IV) enhanced the expression of a gene pivotal for the innate immunity in C. elegans. The study found that the pathogen-resistant phenotypes contributed by Se(IV) was absent from the skn-1 mutant worms. Moreover, Se(IV) influenced the subcellular distribution of SKN-1/Nrf in C. elegans upon PA14 infection. Furthermore, Se(IV) increased mRNA levels of SKN-1 target genes (gst-4 and gcs-1). CONCLUSIONS: This study found evidence of Se(IV) protecting C. elegans against P. aeruginosa PA14 infection by exerting effects on the innate immunity of C. elegans that is likely mediated via regulation of a SKN-1-dependent signaling pathway. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25147937/Selenite_enhances_immune_response_against_Pseudomonas_aeruginosa_PA14_via_SKN_1_in_Caenorhabditis_elegans_ DB - PRIME DP - Unbound Medicine ER -