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Magnetic solid phase extraction coupled with in situ derivatization for the highly sensitive determination of acidic phytohormones in rice leaves by UPLC-MS/MS.
Analyst. 2014 Nov 07; 139(21):5605-13.A

Abstract

A simple, rapid and sensitive method based on magnetic solid phase extraction (MSPE) coupled with in situ derivatization (ISD) was developed for the determination of endogenous acidic phytohormones in rice leaves by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. With this method, acidic phytohormones were extracted onto the surface of a TiO2/magnetic hollow mesoporous silica sphere (MHMSS) through hydrophilic interaction, and then in situ derivatization was performed by the subsequent addition of 3-bromoactonyltrimethylammonium bromide (BTA). Thus, the process integrated extraction, purification, and derivatization into one step. Additionally, the permanent positively charged moiety from BTA significantly improved the ionization efficiencies of the acidic phytohormones. Several parameters affecting the efficiencies of the extraction, derivatization, and desorption were evaluated. The signal intensities of acidic phytohormones increased by 2 to 481 fold after treatment with MSPE-ISD. Under the optimized conditions, several endogenous acidic phytohormones, including GA4, GA9, GA20, JA, IAA, and ABA, were identified and quantified in rice leaves by the MSPE-ISD method. The limits of detection (LODs) were in the range of 1.03-91.21 pg mL(-1). The relative recoveries ranged from 71.6-112.8%, with the intra- and interday relative standard deviations (RSDs) being less than 14.9% and 16.2%, respectively. Taken together, the proposed method provides a novel approach of combining magnetic solid phase extraction and in situ derivatization for the highly sensitive determination of endogenous acidic phytohormones.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P.R. China. yqfeng@whu.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25148576

Citation

Liu, Jiu-Feng, et al. "Magnetic Solid Phase Extraction Coupled With in Situ Derivatization for the Highly Sensitive Determination of Acidic Phytohormones in Rice Leaves By UPLC-MS/MS." The Analyst, vol. 139, no. 21, 2014, pp. 5605-13.
Liu JF, Ding J, Yuan BF, et al. Magnetic solid phase extraction coupled with in situ derivatization for the highly sensitive determination of acidic phytohormones in rice leaves by UPLC-MS/MS. Analyst. 2014;139(21):5605-13.
Liu, J. F., Ding, J., Yuan, B. F., & Feng, Y. Q. (2014). Magnetic solid phase extraction coupled with in situ derivatization for the highly sensitive determination of acidic phytohormones in rice leaves by UPLC-MS/MS. The Analyst, 139(21), 5605-13. https://doi.org/10.1039/c4an01186d
Liu JF, et al. Magnetic Solid Phase Extraction Coupled With in Situ Derivatization for the Highly Sensitive Determination of Acidic Phytohormones in Rice Leaves By UPLC-MS/MS. Analyst. 2014 Nov 7;139(21):5605-13. PubMed PMID: 25148576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Magnetic solid phase extraction coupled with in situ derivatization for the highly sensitive determination of acidic phytohormones in rice leaves by UPLC-MS/MS. AU - Liu,Jiu-Feng, AU - Ding,Jun, AU - Yuan,Bi-Feng, AU - Feng,Yu-Qi, PY - 2014/8/23/entrez PY - 2014/8/26/pubmed PY - 2015/8/25/medline SP - 5605 EP - 13 JF - The Analyst JO - Analyst VL - 139 IS - 21 N2 - A simple, rapid and sensitive method based on magnetic solid phase extraction (MSPE) coupled with in situ derivatization (ISD) was developed for the determination of endogenous acidic phytohormones in rice leaves by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. With this method, acidic phytohormones were extracted onto the surface of a TiO2/magnetic hollow mesoporous silica sphere (MHMSS) through hydrophilic interaction, and then in situ derivatization was performed by the subsequent addition of 3-bromoactonyltrimethylammonium bromide (BTA). Thus, the process integrated extraction, purification, and derivatization into one step. Additionally, the permanent positively charged moiety from BTA significantly improved the ionization efficiencies of the acidic phytohormones. Several parameters affecting the efficiencies of the extraction, derivatization, and desorption were evaluated. The signal intensities of acidic phytohormones increased by 2 to 481 fold after treatment with MSPE-ISD. Under the optimized conditions, several endogenous acidic phytohormones, including GA4, GA9, GA20, JA, IAA, and ABA, were identified and quantified in rice leaves by the MSPE-ISD method. The limits of detection (LODs) were in the range of 1.03-91.21 pg mL(-1). The relative recoveries ranged from 71.6-112.8%, with the intra- and interday relative standard deviations (RSDs) being less than 14.9% and 16.2%, respectively. Taken together, the proposed method provides a novel approach of combining magnetic solid phase extraction and in situ derivatization for the highly sensitive determination of endogenous acidic phytohormones. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/25148576/Magnetic_solid_phase_extraction_coupled_with_in_situ_derivatization_for_the_highly_sensitive_determination_of_acidic_phytohormones_in_rice_leaves_by_UPLC_MS/MS_ DB - PRIME DP - Unbound Medicine ER -