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Dietary iron supplements and Moringa oleifera leaves influence the liver hepcidin messenger RNA expression and biochemical indices of iron status in rats.
Nutr Res. 2014 Jul; 34(7):630-8.NR

Abstract

In this study, the effects of iron depletion and repletion on biochemical and molecular indices of iron status were investigated in growing male Wistar rats. We hypothesized that iron from Moringa leaves could overcome the effects of iron deficiency and modulate the expression of iron-responsive genes better than conventional iron supplements. Iron deficiency was induced by feeding rats an iron-deficient diet for 10 weeks, whereas control rats were maintained on an iron-sufficient diet (35.0-mg Fe/kg diet). After the depletion period, animals were repleted with different source of iron, in combination with ascorbic acid. Iron deficiency caused a significant (P < .05) decrease in serum iron and ferritin levels by 57% and 40%, respectively, as compared with nondepleted control animals. Significant changes in the expression (0.5- to100-fold) of liver hepcidin (HAMP), transferrin, transferrin receptor-2, hemochromatosis type 2, ferroportin 1, ceruloplasmin, and ferritin-H were recorded in iron-depleted and iron-repleted rats, as compared with nondepleted rats (P < .05). Dietary iron from Moringa leaf was found to be superior compared with ferric citrate in overcoming the effects of iron deficiency in rats. These results suggest that changes in the relative expression of liver hepcidin messenger RNA can be used as a sensitive molecular marker for iron deficiency.

Authors+Show Affiliations

Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore 570 020, India.Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore 570 020, India.Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore 570 020, India.Biochemistry & Nutrition Department, CSIR-Central Food Technological Research Institute, Mysore 570 020, India.Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore 570 020, India. Electronic address: pcbt@cftri.res.in.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25150122

Citation

Saini, R K., et al. "Dietary Iron Supplements and Moringa Oleifera Leaves Influence the Liver Hepcidin Messenger RNA Expression and Biochemical Indices of Iron Status in Rats." Nutrition Research (New York, N.Y.), vol. 34, no. 7, 2014, pp. 630-8.
Saini RK, Manoj P, Shetty NP, et al. Dietary iron supplements and Moringa oleifera leaves influence the liver hepcidin messenger RNA expression and biochemical indices of iron status in rats. Nutr Res. 2014;34(7):630-8.
Saini, R. K., Manoj, P., Shetty, N. P., Srinivasan, K., & Giridhar, P. (2014). Dietary iron supplements and Moringa oleifera leaves influence the liver hepcidin messenger RNA expression and biochemical indices of iron status in rats. Nutrition Research (New York, N.Y.), 34(7), 630-8. https://doi.org/10.1016/j.nutres.2014.07.003
Saini RK, et al. Dietary Iron Supplements and Moringa Oleifera Leaves Influence the Liver Hepcidin Messenger RNA Expression and Biochemical Indices of Iron Status in Rats. Nutr Res. 2014;34(7):630-8. PubMed PMID: 25150122.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary iron supplements and Moringa oleifera leaves influence the liver hepcidin messenger RNA expression and biochemical indices of iron status in rats. AU - Saini,R K, AU - Manoj,P, AU - Shetty,N P, AU - Srinivasan,K, AU - Giridhar,P, Y1 - 2014/07/08/ PY - 2014/03/04/received PY - 2014/05/07/revised PY - 2014/07/01/accepted PY - 2014/8/24/entrez PY - 2014/8/26/pubmed PY - 2015/4/15/medline KW - Gene expression KW - Iron deficiency KW - Iron repletion KW - Liver hepcidin KW - Moringa leaves KW - Wistar rats SP - 630 EP - 8 JF - Nutrition research (New York, N.Y.) JO - Nutr Res VL - 34 IS - 7 N2 - In this study, the effects of iron depletion and repletion on biochemical and molecular indices of iron status were investigated in growing male Wistar rats. We hypothesized that iron from Moringa leaves could overcome the effects of iron deficiency and modulate the expression of iron-responsive genes better than conventional iron supplements. Iron deficiency was induced by feeding rats an iron-deficient diet for 10 weeks, whereas control rats were maintained on an iron-sufficient diet (35.0-mg Fe/kg diet). After the depletion period, animals were repleted with different source of iron, in combination with ascorbic acid. Iron deficiency caused a significant (P < .05) decrease in serum iron and ferritin levels by 57% and 40%, respectively, as compared with nondepleted control animals. Significant changes in the expression (0.5- to100-fold) of liver hepcidin (HAMP), transferrin, transferrin receptor-2, hemochromatosis type 2, ferroportin 1, ceruloplasmin, and ferritin-H were recorded in iron-depleted and iron-repleted rats, as compared with nondepleted rats (P < .05). Dietary iron from Moringa leaf was found to be superior compared with ferric citrate in overcoming the effects of iron deficiency in rats. These results suggest that changes in the relative expression of liver hepcidin messenger RNA can be used as a sensitive molecular marker for iron deficiency. SN - 1879-0739 UR - https://www.unboundmedicine.com/medline/citation/25150122/Dietary_iron_supplements_and_Moringa_oleifera_leaves_influence_the_liver_hepcidin_messenger_RNA_expression_and_biochemical_indices_of_iron_status_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0271-5317(14)00100-6 DB - PRIME DP - Unbound Medicine ER -