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Diet type dictates the gut microbiota and the immune response against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss).
Fish Shellfish Immunol. 2014 Oct; 40(2):624-33.FS

Abstract

This study investigated the influence of the rainbow trout (Oncorhynchus mykiss) commensal intestinal microbiota in connection to an experimental Yersina ruckeri infection, the causative agent of enteric redmouth disease. One marine and one plant diet was administered to two different groups of rainbow trout. The plant-based diet gave rise to an intestinal microbiota dominated by the genera Streptococcus, Leuconostoc and Weissella from phylum Firmicutes whereas phylum Proteobacteria/Bacteroidetes/Actinobacteria dominated the community in the marine fed fish. In connection to the Y. ruckeri bath challenge there was no effect of the diet type on the cumulative survival, but the number of Y. ruckeri positive fish as measured by plate count and the number of fish with a 'high' number of reads belonging to genus Yersinia as measured by 16S rRNA next-generation sequencing was higher for marine diet fed fish. Furthermore, the two experimental groups of fish showed a differential immune response, where Y. ruckeri challenged marine fed fish had a higher transcription of IL-1β and MBL-2 relative to challenged plant diet fed fish. The data suggest that the plant diet gave rise to a prebiotic effect favouring the presence of bacterial taxons proving protective in connection to bath challenge by Y. ruckeri.

Authors+Show Affiliations

National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1870 Frederiksberg C, Denmark.National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1870 Frederiksberg C, Denmark.Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 7, DK-1870 Frederiksberg C, Denmark.National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1870 Frederiksberg C, Denmark.National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1870 Frederiksberg C, Denmark.National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1870 Frederiksberg C, Denmark. Electronic address: loma@vet.dtu.dk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25150450

Citation

Ingerslev, Hans-Christian, et al. "Diet Type Dictates the Gut Microbiota and the Immune Response Against Yersinia Ruckeri in Rainbow Trout (Oncorhynchus Mykiss)." Fish & Shellfish Immunology, vol. 40, no. 2, 2014, pp. 624-33.
Ingerslev HC, Strube ML, Jørgensen Lv, et al. Diet type dictates the gut microbiota and the immune response against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol. 2014;40(2):624-33.
Ingerslev, H. C., Strube, M. L., Jørgensen, L. v., Dalsgaard, I., Boye, M., & Madsen, L. (2014). Diet type dictates the gut microbiota and the immune response against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology, 40(2), 624-33. https://doi.org/10.1016/j.fsi.2014.08.021
Ingerslev HC, et al. Diet Type Dictates the Gut Microbiota and the Immune Response Against Yersinia Ruckeri in Rainbow Trout (Oncorhynchus Mykiss). Fish Shellfish Immunol. 2014;40(2):624-33. PubMed PMID: 25150450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diet type dictates the gut microbiota and the immune response against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). AU - Ingerslev,Hans-Christian, AU - Strube,Mikael Lenz, AU - Jørgensen,Louise von Gersdorff, AU - Dalsgaard,Inger, AU - Boye,Mette, AU - Madsen,Lone, Y1 - 2014/08/21/ PY - 2014/05/05/received PY - 2014/08/10/revised PY - 2014/08/12/accepted PY - 2014/8/25/entrez PY - 2014/8/26/pubmed PY - 2015/5/20/medline KW - 16S rRNA KW - Gut microbiota KW - Immune gene transcription KW - Lactobacillaceae KW - Yersinia ruckeri SP - 624 EP - 33 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 40 IS - 2 N2 - This study investigated the influence of the rainbow trout (Oncorhynchus mykiss) commensal intestinal microbiota in connection to an experimental Yersina ruckeri infection, the causative agent of enteric redmouth disease. One marine and one plant diet was administered to two different groups of rainbow trout. The plant-based diet gave rise to an intestinal microbiota dominated by the genera Streptococcus, Leuconostoc and Weissella from phylum Firmicutes whereas phylum Proteobacteria/Bacteroidetes/Actinobacteria dominated the community in the marine fed fish. In connection to the Y. ruckeri bath challenge there was no effect of the diet type on the cumulative survival, but the number of Y. ruckeri positive fish as measured by plate count and the number of fish with a 'high' number of reads belonging to genus Yersinia as measured by 16S rRNA next-generation sequencing was higher for marine diet fed fish. Furthermore, the two experimental groups of fish showed a differential immune response, where Y. ruckeri challenged marine fed fish had a higher transcription of IL-1β and MBL-2 relative to challenged plant diet fed fish. The data suggest that the plant diet gave rise to a prebiotic effect favouring the presence of bacterial taxons proving protective in connection to bath challenge by Y. ruckeri. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/25150450/Diet_type_dictates_the_gut_microbiota_and_the_immune_response_against_Yersinia_ruckeri_in_rainbow_trout__Oncorhynchus_mykiss__ DB - PRIME DP - Unbound Medicine ER -