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Serum metabolites from walnut-fed aged rats attenuate stress-induced neurotoxicity in BV-2 microglial cells.
Nutr Neurosci. 2017 Feb; 20(2):103-109.NN

Abstract

The shift in equilibrium towards excess reactive oxygen or nitrogen species production from innate antioxidant defenses in brain is a critical factor in the declining neural function and cognitive deficit accompanying age. Previous studies from our laboratory have reported that walnuts, rich in polyphenols, antioxidants, and omega fatty acids such as alpha-linolenic acid and linoleic acid, improve the age-associated declines in cognition and neural function in rats. Possible mechanisms of action of these effects include enhancing protective signaling, altering membrane microstructures, decreasing inflammation, and preventing accumulation of polyubiquitinated protein aggregates in critical regions of the brain. In the current study, we investigated whether the serum collected from aged animals fed with walnut diets (0, 6, and 9%, w/w) would enhance protection on stressed BV-2 microglia in vitro. In the growth medium, fetal bovine serum was substituted with the serum collected from 22-month-old rats fed per protocol for 12 weeks. Walnut diet serum (6 and 9%) significantly attenuated lipopolysaccharide-induced nitrite release compared to untreated control cells and those treated with serum from rats fed 0% walnut diets. The results also indicated a significant reduction in pro-inflammatory tumor necrosis factor-alpha, cyclooxygenase-2, and inducible nitric oxide synthase. These results suggest antioxidant and anti-inflammatory protection or enhancement of membrane-associated functions in brain cells by walnut serum metabolites.

Authors+Show Affiliations

a United States Department of Agriculture - Agricultural Research Services , Human Nutrition Research Center on Aging, Tufts University , Boston , MA , USA.a United States Department of Agriculture - Agricultural Research Services , Human Nutrition Research Center on Aging, Tufts University , Boston , MA , USA.a United States Department of Agriculture - Agricultural Research Services , Human Nutrition Research Center on Aging, Tufts University , Boston , MA , USA.a United States Department of Agriculture - Agricultural Research Services , Human Nutrition Research Center on Aging, Tufts University , Boston , MA , USA.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

25153536

Citation

Fisher, Derek R., et al. "Serum Metabolites From Walnut-fed Aged Rats Attenuate Stress-induced Neurotoxicity in BV-2 Microglial Cells." Nutritional Neuroscience, vol. 20, no. 2, 2017, pp. 103-109.
Fisher DR, Poulose SM, Bielinski DF, et al. Serum metabolites from walnut-fed aged rats attenuate stress-induced neurotoxicity in BV-2 microglial cells. Nutr Neurosci. 2017;20(2):103-109.
Fisher, D. R., Poulose, S. M., Bielinski, D. F., & Shukitt-Hale, B. (2017). Serum metabolites from walnut-fed aged rats attenuate stress-induced neurotoxicity in BV-2 microglial cells. Nutritional Neuroscience, 20(2), 103-109. https://doi.org/10.1179/1476830514Y.0000000150
Fisher DR, et al. Serum Metabolites From Walnut-fed Aged Rats Attenuate Stress-induced Neurotoxicity in BV-2 Microglial Cells. Nutr Neurosci. 2017;20(2):103-109. PubMed PMID: 25153536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum metabolites from walnut-fed aged rats attenuate stress-induced neurotoxicity in BV-2 microglial cells. AU - Fisher,Derek R, AU - Poulose,Shibu M, AU - Bielinski,Donna F, AU - Shukitt-Hale,Barbara, Y1 - 2016/03/02/ PY - 2014/8/26/pubmed PY - 2017/3/14/medline PY - 2014/8/26/entrez KW - Inflammation KW - Nitrite KW - Omega fatty acids KW - Oxidative stress SP - 103 EP - 109 JF - Nutritional neuroscience JO - Nutr Neurosci VL - 20 IS - 2 N2 - The shift in equilibrium towards excess reactive oxygen or nitrogen species production from innate antioxidant defenses in brain is a critical factor in the declining neural function and cognitive deficit accompanying age. Previous studies from our laboratory have reported that walnuts, rich in polyphenols, antioxidants, and omega fatty acids such as alpha-linolenic acid and linoleic acid, improve the age-associated declines in cognition and neural function in rats. Possible mechanisms of action of these effects include enhancing protective signaling, altering membrane microstructures, decreasing inflammation, and preventing accumulation of polyubiquitinated protein aggregates in critical regions of the brain. In the current study, we investigated whether the serum collected from aged animals fed with walnut diets (0, 6, and 9%, w/w) would enhance protection on stressed BV-2 microglia in vitro. In the growth medium, fetal bovine serum was substituted with the serum collected from 22-month-old rats fed per protocol for 12 weeks. Walnut diet serum (6 and 9%) significantly attenuated lipopolysaccharide-induced nitrite release compared to untreated control cells and those treated with serum from rats fed 0% walnut diets. The results also indicated a significant reduction in pro-inflammatory tumor necrosis factor-alpha, cyclooxygenase-2, and inducible nitric oxide synthase. These results suggest antioxidant and anti-inflammatory protection or enhancement of membrane-associated functions in brain cells by walnut serum metabolites. SN - 1476-8305 UR - https://www.unboundmedicine.com/medline/citation/25153536/Serum_metabolites_from_walnut_fed_aged_rats_attenuate_stress_induced_neurotoxicity_in_BV_2_microglial_cells_ L2 - https://www.tandfonline.com/doi/full/10.1179/1476830514Y.0000000150 DB - PRIME DP - Unbound Medicine ER -