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Photoelectrochemical biosensor using enzyme-catalyzed in situ propagation of CdS quantum dots on graphene oxide.
ACS Appl Mater Interfaces. 2014 Sep 24; 6(18):16197-203.AA

Abstract

An innovative photoelectrochemical (PEC) biosensor platform was designed based on the in situ generation of CdS quantum dots (QDs) on graphene oxide (GO) using an enzymatic reaction. Horseradish peroxidase catalyzed the reduction of sodium thiosulfate with hydrogen peroxide to generate H2S, which reacted with Cd(2+) to form CdS QDs. CdS QDs could be photoexcited to generate an elevated photocurrent as a readout signal. This strategy offered a "green" alternative to inconvenient presynthesis procedures for the fabrication of semiconducting nanoparticles. The nanomaterials and assembly procedures were characterized by microscopy and spectroscopy techniques. Combined with immune recognition and on the basis of the PEC activity of CdS QDs on GO, the strategy was successfully applied to a PEC assay to detect carcinoembryonic antigen and displayed a wide linear range from 2.5 ng mL(-1) to 50 μg mL(-1) and a detection limit of 0.72 ng mL(-1) at a signal-to-noise ratio of 3. The PEC biosensor showed satisfactory performance for clinical sample detection and was convenient for determining high concentrations of solute without dilution. This effort offers a new opportunity for the development of numerous rapid and convenient analytical techniques using the PEC method that may be applied in the design and preparation of various solar-energy-driven applications.

Authors+Show Affiliations

Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing, 210023 Jiangsu, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25154012

Citation

Zeng, Xianxiang, et al. "Photoelectrochemical Biosensor Using Enzyme-catalyzed in Situ Propagation of CdS Quantum Dots On Graphene Oxide." ACS Applied Materials & Interfaces, vol. 6, no. 18, 2014, pp. 16197-203.
Zeng X, Tu W, Li J, et al. Photoelectrochemical biosensor using enzyme-catalyzed in situ propagation of CdS quantum dots on graphene oxide. ACS Appl Mater Interfaces. 2014;6(18):16197-203.
Zeng, X., Tu, W., Li, J., Bao, J., & Dai, Z. (2014). Photoelectrochemical biosensor using enzyme-catalyzed in situ propagation of CdS quantum dots on graphene oxide. ACS Applied Materials & Interfaces, 6(18), 16197-203. https://doi.org/10.1021/am5043164
Zeng X, et al. Photoelectrochemical Biosensor Using Enzyme-catalyzed in Situ Propagation of CdS Quantum Dots On Graphene Oxide. ACS Appl Mater Interfaces. 2014 Sep 24;6(18):16197-203. PubMed PMID: 25154012.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photoelectrochemical biosensor using enzyme-catalyzed in situ propagation of CdS quantum dots on graphene oxide. AU - Zeng,Xianxiang, AU - Tu,Wenwen, AU - Li,Jing, AU - Bao,Jianchun, AU - Dai,Zhihui, Y1 - 2014/09/08/ PY - 2014/8/26/entrez PY - 2014/8/26/pubmed PY - 2015/10/22/medline KW - biosensing KW - enzyme catalysis KW - graphene oxide KW - photoelectrochemistry KW - quantum dots SP - 16197 EP - 203 JF - ACS applied materials & interfaces JO - ACS Appl Mater Interfaces VL - 6 IS - 18 N2 - An innovative photoelectrochemical (PEC) biosensor platform was designed based on the in situ generation of CdS quantum dots (QDs) on graphene oxide (GO) using an enzymatic reaction. Horseradish peroxidase catalyzed the reduction of sodium thiosulfate with hydrogen peroxide to generate H2S, which reacted with Cd(2+) to form CdS QDs. CdS QDs could be photoexcited to generate an elevated photocurrent as a readout signal. This strategy offered a "green" alternative to inconvenient presynthesis procedures for the fabrication of semiconducting nanoparticles. The nanomaterials and assembly procedures were characterized by microscopy and spectroscopy techniques. Combined with immune recognition and on the basis of the PEC activity of CdS QDs on GO, the strategy was successfully applied to a PEC assay to detect carcinoembryonic antigen and displayed a wide linear range from 2.5 ng mL(-1) to 50 μg mL(-1) and a detection limit of 0.72 ng mL(-1) at a signal-to-noise ratio of 3. The PEC biosensor showed satisfactory performance for clinical sample detection and was convenient for determining high concentrations of solute without dilution. This effort offers a new opportunity for the development of numerous rapid and convenient analytical techniques using the PEC method that may be applied in the design and preparation of various solar-energy-driven applications. SN - 1944-8252 UR - https://www.unboundmedicine.com/medline/citation/25154012/Photoelectrochemical_biosensor_using_enzyme_catalyzed_in_situ_propagation_of_CdS_quantum_dots_on_graphene_oxide_ L2 - https://doi.org/10.1021/am5043164 DB - PRIME DP - Unbound Medicine ER -