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Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia.
Chin J Nat Med. 2014 Aug; 12(8):573-81.CJ

Abstract

AIM

To investigate the anti-inflammatory activities of the semen extract of Cuscuta chinensis Lam. (Cuscutae Semen; CS) on the production of inflammatory mediators, nitric oxide (NO), prostaglandin 2 (PGE2), and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV-2 microglia.

METHOD

BV-2 cells were treated with CS extract for 30 min, and then stimulated with LPS or without for 24 h. The levels of NO, PGE2 and proinflammatory cytokines were measured by Griess assay and ELISA. The expression of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. The phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK), and the nuclear expression of nuclear factor (NF)-κB p65 were investigated by Western blot analysis.

RESULTS

CS extract significantly decreased the production of NO and PGE2 by suppressing the expression of iNOS and COX-2 in activated microglia. CS extract decreased the production of TNF-α, IL-1β, and IL-6 by down-regulating their transcription levels. In addition, CS extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-κB p65 in activated microglia.

CONCLUSION

These results indicate that CS extract is capable of suppressing the inflammatory response by microglia activation, suggesting that CS extract has potential in the treatment of brain inflammation.

Authors+Show Affiliations

Department of Herbology, College of Korean Medicine, Dongguk University, 707, Sekjang-Dong, Gyeongju, Gyeongsangbuk-Do, 780-714, Republic of Korea.Department of Herbology, College of Korean Medicine, Dongguk University, 707, Sekjang-Dong, Gyeongju, Gyeongsangbuk-Do, 780-714, Republic of Korea; Korean Medicine R&D Center, Dongguk University, 707, Sekjang-Dong, Gyeongju, Gyeongsangbuk-Do, 780-714, Republic of Korea.Korea Institute of Oriental Medicine, 1672, Yuseongdae-ro, Yuseong-gu, Daejeon, 305-811, Republic of Korea.Korea Institute of Oriental Medicine, 1672, Yuseongdae-ro, Yuseong-gu, Daejeon, 305-811, Republic of Korea.Korea Institute of Oriental Medicine, 1672, Yuseongdae-ro, Yuseong-gu, Daejeon, 305-811, Republic of Korea.Department of Herbology, College of Korean Medicine, Dongguk University, 707, Sekjang-Dong, Gyeongju, Gyeongsangbuk-Do, 780-714, Republic of Korea; Korean Medicine R&D Center, Dongguk University, 707, Sekjang-Dong, Gyeongju, Gyeongsangbuk-Do, 780-714, Republic of Korea. Electronic address: yongki@dongguk.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25156282

Citation

Kang, Seok Yong, et al. "Effect of the Semen Extract of Cuscuta Chinensis On Inflammatory Responses in LPS-stimulated BV-2 Microglia." Chinese Journal of Natural Medicines, vol. 12, no. 8, 2014, pp. 573-81.
Kang SY, Jung HW, Lee MY, et al. Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia. Chin J Nat Med. 2014;12(8):573-81.
Kang, S. Y., Jung, H. W., Lee, M. Y., Lee, H. W., Chae, S. W., & Park, Y. K. (2014). Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia. Chinese Journal of Natural Medicines, 12(8), 573-81. https://doi.org/10.1016/S1875-5364(14)60088-1
Kang SY, et al. Effect of the Semen Extract of Cuscuta Chinensis On Inflammatory Responses in LPS-stimulated BV-2 Microglia. Chin J Nat Med. 2014;12(8):573-81. PubMed PMID: 25156282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia. AU - Kang,Seok Yong, AU - Jung,Hyo Won, AU - Lee,Mi-Young, AU - Lee,Hye Won, AU - Chae,Seong Wook, AU - Park,Yong-Ki, PY - 2013/03/11/received PY - 2014/8/27/entrez PY - 2014/8/27/pubmed PY - 2015/5/13/medline KW - Cuscuta chinensis KW - Cuscutae Semen KW - Inflammation KW - Microglia KW - NF-κB pathway SP - 573 EP - 81 JF - Chinese journal of natural medicines JO - Chin J Nat Med VL - 12 IS - 8 N2 - AIM: To investigate the anti-inflammatory activities of the semen extract of Cuscuta chinensis Lam. (Cuscutae Semen; CS) on the production of inflammatory mediators, nitric oxide (NO), prostaglandin 2 (PGE2), and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV-2 microglia. METHOD: BV-2 cells were treated with CS extract for 30 min, and then stimulated with LPS or without for 24 h. The levels of NO, PGE2 and proinflammatory cytokines were measured by Griess assay and ELISA. The expression of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. The phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK), and the nuclear expression of nuclear factor (NF)-κB p65 were investigated by Western blot analysis. RESULTS: CS extract significantly decreased the production of NO and PGE2 by suppressing the expression of iNOS and COX-2 in activated microglia. CS extract decreased the production of TNF-α, IL-1β, and IL-6 by down-regulating their transcription levels. In addition, CS extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-κB p65 in activated microglia. CONCLUSION: These results indicate that CS extract is capable of suppressing the inflammatory response by microglia activation, suggesting that CS extract has potential in the treatment of brain inflammation. SN - 1875-5364 UR - https://www.unboundmedicine.com/medline/citation/25156282/Effect_of_the_semen_extract_of_Cuscuta_chinensis_on_inflammatory_responses_in_LPS_stimulated_BV_2_microglia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1875-5364(14)60088-1 DB - PRIME DP - Unbound Medicine ER -