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N-acetylcysteine administration confers lung protection in different phases of lung ischaemia-reperfusion injury.
Interact Cardiovasc Thorac Surg. 2014 Dec; 19(6):894-9.IC

Abstract

OBJECTIVES

To verify the effects of N-acetylcysteine (NAC) administered before and after ischaemia in an animal model of lung ischaemia-reperfusion (IR) injury.

METHODS

Twenty-four Wistar rats were subjected to an experimental model of selective left pulmonary hilar clamping for 45 min followed by 2 h of reperfusion. The animals were divided into four groups: control group (SHAM), ischaemia-reperfusion, N-acetylcysteine-preischaemia (NAC-Pre) and NAC-postischaemia (NAC-Post). We recorded the haemodynamic parameters, blood gas analysis and histology. We measured the thiobarbituric acid reactive substances concentration; the expression of superoxide dismutase (SOD), inducible nitric oxide synthase (iNOS), nitrotyrosine, cleaved caspase 3, nuclear factor κB (NF-κB), NF-kappa-B inhibitor alpha (IκB-α), tumour necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β); myeloperoxidase activity (MPO).

RESULTS

No significant differences were observed in the haemodynamic parameters, blood gas analysis and SOD activity among the groups. Lipid peroxidation was significantly higher in the IR and NAC-Pre groups (P < 0.01). The expression of nitrotyrosine, cleaved caspase 3, NF-κB, IκB-α, TNF-α and IL-1β were significantly higher in the IR group when compared with the SHAM and NAC groups (P < 0.01). The NAC-Pre group showed a significantly higher expression of these proteins when compared with the SHAM and NAC-Post groups (P < 0.05). After reperfusion, the expression of iNOS increased almost uniformly in all groups when compared with the SHAM group (P < 0.01). The histological analysis showed fewer inflammatory cells in the NAC groups.

CONCLUSIONS

The intravenous administration of NAC demonstrated protective properties against lung IR injury. The use of NAC immediately after reperfusion potentiates its protective effects.

Authors+Show Affiliations

Postgraduate Program of Pulmonology (Federal University of Rio Grande do Sul - UFRGS) Department of Thoracic Surgery, Laboratory of Airway and Lung, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.Department of Thoracic Surgery, Laboratory of Airway and Lung, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.Department of Thoracic Surgery, Laboratory of Airway and Lung, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.Department of Thoracic Surgery, Laboratory of Airway and Lung, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.Department of Thoracic Surgery, Laboratory of Airway and Lung, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.Department of Thoracic Surgery, Laboratory of Airway and Lung, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.Department of Thoracic Surgery, Laboratory of Airway and Lung, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil cristianofa@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25156898

Citation

Forgiarini, Luiz Felipe, et al. "N-acetylcysteine Administration Confers Lung Protection in Different Phases of Lung Ischaemia-reperfusion Injury." Interactive Cardiovascular and Thoracic Surgery, vol. 19, no. 6, 2014, pp. 894-9.
Forgiarini LF, Forgiarini LA, da Rosa DP, et al. N-acetylcysteine administration confers lung protection in different phases of lung ischaemia-reperfusion injury. Interact Cardiovasc Thorac Surg. 2014;19(6):894-9.
Forgiarini, L. F., Forgiarini, L. A., da Rosa, D. P., Silva, M. B., Mariano, R., Paludo, A. d. e. . O., & Andrade, C. F. (2014). N-acetylcysteine administration confers lung protection in different phases of lung ischaemia-reperfusion injury. Interactive Cardiovascular and Thoracic Surgery, 19(6), 894-9. https://doi.org/10.1093/icvts/ivu258
Forgiarini LF, et al. N-acetylcysteine Administration Confers Lung Protection in Different Phases of Lung Ischaemia-reperfusion Injury. Interact Cardiovasc Thorac Surg. 2014;19(6):894-9. PubMed PMID: 25156898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-acetylcysteine administration confers lung protection in different phases of lung ischaemia-reperfusion injury. AU - Forgiarini,Luiz Felipe, AU - Forgiarini,Luiz Alberto,Jr AU - da Rosa,Darlan Pase, AU - Silva,Mariel Barbachan E, AU - Mariano,Rodrigo, AU - Paludo,Artur de Oliveira, AU - Andrade,Cristiano Feijó, Y1 - 2014/08/24/ PY - 2014/8/27/entrez PY - 2014/8/27/pubmed PY - 2016/3/24/medline KW - Inflammation KW - Ischaemia–reperfusion KW - N-Acetylcysteine SP - 894 EP - 9 JF - Interactive cardiovascular and thoracic surgery JO - Interact Cardiovasc Thorac Surg VL - 19 IS - 6 N2 - OBJECTIVES: To verify the effects of N-acetylcysteine (NAC) administered before and after ischaemia in an animal model of lung ischaemia-reperfusion (IR) injury. METHODS: Twenty-four Wistar rats were subjected to an experimental model of selective left pulmonary hilar clamping for 45 min followed by 2 h of reperfusion. The animals were divided into four groups: control group (SHAM), ischaemia-reperfusion, N-acetylcysteine-preischaemia (NAC-Pre) and NAC-postischaemia (NAC-Post). We recorded the haemodynamic parameters, blood gas analysis and histology. We measured the thiobarbituric acid reactive substances concentration; the expression of superoxide dismutase (SOD), inducible nitric oxide synthase (iNOS), nitrotyrosine, cleaved caspase 3, nuclear factor κB (NF-κB), NF-kappa-B inhibitor alpha (IκB-α), tumour necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β); myeloperoxidase activity (MPO). RESULTS: No significant differences were observed in the haemodynamic parameters, blood gas analysis and SOD activity among the groups. Lipid peroxidation was significantly higher in the IR and NAC-Pre groups (P < 0.01). The expression of nitrotyrosine, cleaved caspase 3, NF-κB, IκB-α, TNF-α and IL-1β were significantly higher in the IR group when compared with the SHAM and NAC groups (P < 0.01). The NAC-Pre group showed a significantly higher expression of these proteins when compared with the SHAM and NAC-Post groups (P < 0.05). After reperfusion, the expression of iNOS increased almost uniformly in all groups when compared with the SHAM group (P < 0.01). The histological analysis showed fewer inflammatory cells in the NAC groups. CONCLUSIONS: The intravenous administration of NAC demonstrated protective properties against lung IR injury. The use of NAC immediately after reperfusion potentiates its protective effects. SN - 1569-9285 UR - https://www.unboundmedicine.com/medline/citation/25156898/N_acetylcysteine_administration_confers_lung_protection_in_different_phases_of_lung_ischaemia_reperfusion_injury_ L2 - https://academic.oup.com/icvts/article-lookup/doi/10.1093/icvts/ivu258 DB - PRIME DP - Unbound Medicine ER -