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Electrogenerated chemiluminescence aptasensor for ultrasensitive detection of thrombin incorporating an auxiliary probe.
Talanta. 2014 Dec; 130:370-6.T

Abstract

A novel electrogenerated chemiluminescence (ECL) aptasensor for ultrasensitive detection of thrombin incorporating an auxiliary probe was designed by employing specific anti-thrombin aptamer as a capture probe and a ruthenium(II) complex-tagged reporter probe as an ECL probe and an auxiliary probe to assist the ECL probe close to the surface of the electrode. The ECL aptasensor was fabricated by self-assembling a thiolated capture probe on the surface of gold electrode and then hybridizing the ECL probe with the capture probe, and further self-assembling the auxiliary probe. When analyte thrombin was bound with the capture probe, the part of the dehybridized ECL probe was hybridized with the neighboring auxiliary probe, led to the tagged ruthenium(II) complex close to the electrode surface, resulted in great increase in the ECL intensity. The results showed that the increased ECL intensity was directly related to the logarithm of thrombin concentrations in the range from 5.0 × 10(-15)M to 5.0 × 10(-12)M with a detection limit of 2.0 × 10(-15)M. This work demonstrates that employing an auxiliary probe which exists nearby the capture probe can enhance the sensitivity of the ECL aptasensor. This promising strategy will be extended to the design of other biosensors for detection of other proteins and genes.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. Electronic address: cxzhang@snnu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25159423

Citation

Li, Zhejian, et al. "Electrogenerated Chemiluminescence Aptasensor for Ultrasensitive Detection of Thrombin Incorporating an Auxiliary Probe." Talanta, vol. 130, 2014, pp. 370-6.
Li Z, Sun L, Zhao Y, et al. Electrogenerated chemiluminescence aptasensor for ultrasensitive detection of thrombin incorporating an auxiliary probe. Talanta. 2014;130:370-6.
Li, Z., Sun, L., Zhao, Y., Yang, L., Qi, H., Gao, Q., & Zhang, C. (2014). Electrogenerated chemiluminescence aptasensor for ultrasensitive detection of thrombin incorporating an auxiliary probe. Talanta, 130, 370-6. https://doi.org/10.1016/j.talanta.2014.07.029
Li Z, et al. Electrogenerated Chemiluminescence Aptasensor for Ultrasensitive Detection of Thrombin Incorporating an Auxiliary Probe. Talanta. 2014;130:370-6. PubMed PMID: 25159423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrogenerated chemiluminescence aptasensor for ultrasensitive detection of thrombin incorporating an auxiliary probe. AU - Li,Zhejian, AU - Sun,Lijuan, AU - Zhao,Ying, AU - Yang,Libin, AU - Qi,Honglan, AU - Gao,Qiang, AU - Zhang,Chengxiao, Y1 - 2014/07/18/ PY - 2014/04/04/received PY - 2014/07/03/revised PY - 2014/07/10/accepted PY - 2014/8/28/entrez PY - 2014/8/28/pubmed PY - 2015/5/13/medline KW - Aptamer KW - Aptasensor KW - Auxiliary probe KW - Electrogenerated chemiluminescence KW - Ruthenium(II) complex KW - Thrombin SP - 370 EP - 6 JF - Talanta JO - Talanta VL - 130 N2 - A novel electrogenerated chemiluminescence (ECL) aptasensor for ultrasensitive detection of thrombin incorporating an auxiliary probe was designed by employing specific anti-thrombin aptamer as a capture probe and a ruthenium(II) complex-tagged reporter probe as an ECL probe and an auxiliary probe to assist the ECL probe close to the surface of the electrode. The ECL aptasensor was fabricated by self-assembling a thiolated capture probe on the surface of gold electrode and then hybridizing the ECL probe with the capture probe, and further self-assembling the auxiliary probe. When analyte thrombin was bound with the capture probe, the part of the dehybridized ECL probe was hybridized with the neighboring auxiliary probe, led to the tagged ruthenium(II) complex close to the electrode surface, resulted in great increase in the ECL intensity. The results showed that the increased ECL intensity was directly related to the logarithm of thrombin concentrations in the range from 5.0 × 10(-15)M to 5.0 × 10(-12)M with a detection limit of 2.0 × 10(-15)M. This work demonstrates that employing an auxiliary probe which exists nearby the capture probe can enhance the sensitivity of the ECL aptasensor. This promising strategy will be extended to the design of other biosensors for detection of other proteins and genes. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/25159423/Electrogenerated_chemiluminescence_aptasensor_for_ultrasensitive_detection_of_thrombin_incorporating_an_auxiliary_probe_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(14)00585-2 DB - PRIME DP - Unbound Medicine ER -